scholarly journals Determination of Failure of Treatment of Plasmodium falciparum Infection by Using Polymerase Chain Reaction Single‐Strand Conformational Polymorphism Fingerprinting

1999 ◽  
Vol 28 (4) ◽  
pp. 847-852 ◽  
Author(s):  
Colin Ohrt ◽  
Laura Mirabelli‐Primdahl ◽  
Sornchai Looareesuwan ◽  
Polrat Wilairatana ◽  
Douglas Walsh ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodium Falciparum ◽  
Falciparum Infection ◽  
Single Strand ◽  
Chain Reaction ◽  
Single Strand Conformational Polymorphism ◽  
Conformational Polymorphism ◽  
Plasmodium Falciparum Infection ◽  
Polymerase Chain

scholarly journals Application of Polymerase Chain Reaction–Single Strand Conformational Polymorphism (PCR–SSCP) to Identification of Flatfish Species

1999 ◽  
Vol 82 (4) ◽  
pp. 903-907 ◽  
Author(s):  
Ana Céspedes ◽  
Teresa García ◽  
Esther Carrera ◽  
Isabel González ◽  
Esther Carrera ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polyacrylamide Gel Electrophoresis ◽  
Single Strand ◽  
Chain Reaction ◽  
Greenland Halibut ◽  
Single Strand Conformational Polymorphism ◽  
Conformational Polymorphism ◽  
Flatfish Species ◽  
Mitochondrial Cytochrome B ◽  
Polymerase Chain

Abstract A method of DNA analysis based on polymerase chain reaction–single strand conformational polymorphism (PCR–SSCP) was developed to verify the authenticity of labeled raw and frozen fillets of some flatfish species. PCR was used to amplify a short fragment (201 bp) of the mitochondrial cytochrome b gene, which was denatured and analyzed by native polyacrylamide gel electrophoresis for detection of SSCPs. Species-specific patterns of DNA bands were obtained for sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides).

Sign in / Sign up

Export Citation Format

Share Document