Determination of Repeat Numbers of (CA)n in Mitochondrial D-loop using Polymerase Chain Reaction-single Strand Conformational Polymorphism (PCR-SSCP)

2018 ◽  
Vol 31 (3) ◽  
pp. 77
Author(s):  
Joo-Young Kim ◽  
Dae-Kwang Kim
Keyword(s):  
Polymerase Chain Reaction ◽  
Single Strand ◽  
Chain Reaction ◽  
Single Strand Conformational Polymorphism ◽  
Conformational Polymorphism ◽  
D Loop ◽  
Polymerase Chain

scholarly journals Application of Polymerase Chain Reaction–Single Strand Conformational Polymorphism (PCR–SSCP) to Identification of Flatfish Species

1999 ◽  
Vol 82 (4) ◽  
pp. 903-907 ◽  
Author(s):  
Ana Céspedes ◽  
Teresa García ◽  
Esther Carrera ◽  
Isabel González ◽  
Esther Carrera ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polyacrylamide Gel Electrophoresis ◽  
Single Strand ◽  
Chain Reaction ◽  
Greenland Halibut ◽  
Single Strand Conformational Polymorphism ◽  
Conformational Polymorphism ◽  
Flatfish Species ◽  
Mitochondrial Cytochrome B ◽  
Polymerase Chain

Abstract A method of DNA analysis based on polymerase chain reaction–single strand conformational polymorphism (PCR–SSCP) was developed to verify the authenticity of labeled raw and frozen fillets of some flatfish species. PCR was used to amplify a short fragment (201 bp) of the mitochondrial cytochrome b gene, which was denatured and analyzed by native polyacrylamide gel electrophoresis for detection of SSCPs. Species-specific patterns of DNA bands were obtained for sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides).

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