scholarly journals Development of STEM tissue culture module in promoting plant biotechnology

2021 ◽  
Vol 1882 (1) ◽  
pp. 012159
Author(s):  
M Md Moid ◽  
N A S S Alam ◽  
I Z A N Rasidi ◽  
N A Suliman ◽  
H H Azman
Keyword(s):  
Tissue Culture ◽  
Plant Biotechnology ◽  
Stem Tissue

Application of Plant Tissue Culture in Management of Hg Contaminated Water

2018 ◽  
pp. 169-183
Author(s):  
Srijan Goswami ◽  
Debraj Modak ◽  
Munmun Modak
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Environmental Contamination ◽  
Plant Tissue ◽  
Plant Biotechnology ◽  
In Vitro Studies ◽  
Mercury Contamination

In the chapter, the authors describe how a plant can be selected as a natural indicator of heavy metal (mercury) contamination, how one can select a plant species to monitor a particular type of heavy metal and use those plants to remove the contaminants from the area under consideration. The chapter also contains a brief idea of environmental contamination by heavy metals and how the situation can be managed by the techniques of modern plant biotechnology. The authors add some reports and data of their in-vitro studies of mercury toxicity on Ananas sp., generally known as pineapple, for better understanding of the text.

scholarly journals Prevention of Fungal Contamination in Plant Tissue Culture Using Cyclic Lipopeptides Secreted by Bacillus amyloliquefaciens AB30a

2019 ◽  
Vol 29 (1) ◽  
pp. 111-119
Author(s):  
Khushboo Rawal ◽  
Hareshkumar Keharia
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Bacillus Amyloliquefaciens ◽  
In Vitro Propagation ◽  
Fungal Growth ◽  
Plant Biotechnology ◽  
Nodal Segment ◽  
Heat Stable

Plant tissue culture has revolutionized the field of plant biotechnology. However, there are certain obstacles which overall restrain the output of the plant tissue culturing. One of them is contamination of the tissue culture stock which is a major problem limiting the output. Aegle marmelos (L.) is a medicinal plant whose genotype qualities are maintained through clonal propagation of nodal segment as an explant. It harbors plethora of fungi which curbs the successful in vitro propagation. Chemical fungicide like bavistin is used to prevent the contamination in tissue culture which raises the environmental concerns. Thus, use of microbially derived antifungals can help in preventing fungal growth with benefit of positively impacting the plant growth. Here, authors investigated the use of heat stable lipopeptides which are secondary metabolites derived from Bacillus amyloliquefaciens AB30a for prevention of contamination in tissue culturing of nodal explants of A. marmelos positively impacting its in-vitro propagation. Plant Tissue Cult. & Biotech. 29(1): 111-119, 2019 (June)

scholarly journals THE GROWTH OF JACKFRUIT (ARTOCARPUS HETEROPHYLLUS L.) SHOOTS ON VARIOUS CONCENTRATION BENZYLAMINO PURINE (BAP) IN VITRO

2019 ◽  
Vol 5 (2) ◽  
pp. 99
Author(s):  
Frisca Nanda Bulo ◽  
Enny Adelina ◽  
Ramal Yusuf ◽  
Hawalina Kasim
Keyword(s):  
Tissue Culture ◽  
Plant Biotechnology ◽  
Culture Technique ◽  
Artocarpus Heterophyllus ◽  
Conventional Breeding ◽  
Resistant Cultivars ◽  
Number Of Leaves ◽  
Central Sulawesi ◽  
Shoot Emergence

The development of Central Sulawesi superior commodity,which is Tulo-5, the drough-resistant cultivars, through tissue culture technique can be used as an alternative, in addition to conventional breeding. This study aims to determine the growth of jackfruit shoots in  various concentrations of benzylamino purine in vitro. The research has been conducted at the Laboratory of Plant Biotechnology, Agriculture Faculty of Tadulako University, Palu.  The research began on December 2017 and ended up on February 2018. This research used Completely Randomised Design of one-factor treatment which is BAP concentrations consist of 4 stage which is1,5 ppm BAP, 2,0 ppm BAP, 2,5 ppm BAP, 3,0 ppm BAP. The result showed that 6 weeks after planting, BAP concentration had significant effect on shoots time to emerge and shoots number but no significant effect on leaf number.  The result of Tukey’s HSD test showed that concentration2,5 ppm gave the fastest shoot emergence with an average of 2,875 days after planting, in concentration 2,0 ppm gave the highest number of shoots with an average of 2.125 shoots per explant, and for the highest number of leaves found in the concentration of 2,0 ppm with the average number of leaves is 1 strand per explant. 

Importance of Artificial Environment Conditions on Plant Biotechnology, Plant Growth, and Secondary Metabolites

2021 ◽  
pp. 292-319
Author(s):  
Ahmed M.M. Gabr ◽  
Hanan S. Ebrahim ◽  
Amal A El-Ashry ◽  
Mohamed K. El-Bahr
Keyword(s):  
Tissue Culture ◽  
Secondary Metabolites ◽  
Plant Growth ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Plant Secondary Metabolites ◽  
Plant Biotechnology ◽  
Artificial Environment ◽  
Metabolites Production

Plant biotechnology (micropropagation, secondary metabolites, etc.) is the science of growing different plant cells under controlled artificial environment conditions. Thence, successful plant biotechnology depends on the techniques of plant tissue culture. Plant tissue culture laboratories have style needs that distinguish them from alternative forms of laboratories and a few wants are distinctive for successfully plant growth as well as production of plant secondary metabolites. The most often manipulated physical parameters are light, temperature, and relative humidity within the culture vessels. The light is most important physical factor which might have an effect on metabolite production. Light not only affects the in vitro growth and developments but also very important factor affecting the production of plant secondary metabolites. The action of light on plants happens in the most part in two ways. In the first way, the light source gives the energy required by the plant for photosynthesis. In the second way, the signal which gotten by photoreceptors it regulates plant metabolism, differentiation, and growth. Temperature is different with the different in vitro life cycle, as well as the aim of production such as plant growth or secondary metabolites production.

scholarly journals PENGUJIAN EFEKTIVITAS BEBERAPA ISOLAT CENDAWAN MIKORIZA ARBUSKULA (CMA) TERHADAP BIBIT PISANG (Musa AAB RAJA NANGKA) ASAL KULTUR JARINGAN

2009 ◽  
Vol 15 (1) ◽  
pp. 63-69
Author(s):  
Rainiyati Rainiyati ◽  
Chozin Chozin ◽  
Sudarsono Sudarsono ◽  
Mansur Mansur
Keyword(s):  
Tissue Culture ◽  
Arbuscular Mycorrhizal Fungi ◽  
Nutrient Uptake ◽  
Mycorrhizal Fungi ◽  
Plant Biotechnology ◽  
Arbuscular Mycorrhizal ◽  
Growth Responses ◽  
Musa Sp ◽  
Glomus Sp

An experiment to investigate the affectivity five isolat of Arbuscular Mycorrhizal Fungi (AMF) on the growth of banana (Musa sp.) obtained from tissue culture had been conducted at the Plant Biotechnology laboratory, Glasshouse, and Experimental farm of the Faculty of Agriculture Jambi University. The results showed that there were five mycorrhizal isolates that can be tested for their affectivity on banana seedlings. The growth responses of the seedlings inoculated with AMF was higher than those non-inoculated seedlings due to higher rate of nutrient uptake. Each kind of AMF possesses different level of effectiveness on banana seedling. The application of single isolate Glomus sp-1 at acclimatization time resulted that highest effectiveness.

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