In vitro
                    osteogenic differentiation of human amniotic fluid-derived stem cells on a poly(lactide-
                    co
                    -glycolide) (PLGA)–bladder submucosa matrix (BSM) composite scaffold for bone tissue engineering

Biocompatible scaffolding materials play an important role in bone tissue engineering. This study sought to develop and characterize a nano-hydroxyapatite (nHA)/collagen I (ColI)/multi-walled carbon nanotube (MWCNT) composite scaffold loaded with recombinant bone morphogenetic protein-9 (BMP-9) for bone tissue engineering by in vitro and in vivo experiments. The composite nHA/ColI/MWCNT scaffolds were fabricated at various concentrations of MWCNTs (0.5, 1, and 1.5% wt) by blending and freeze drying. The porosity, swelling rate, water absorption rate, mechanical properties, and biocompatibility of scaffolds were measured. After loading with BMP-9, bone marrow mesenchymal stem cells (BMMSCs) were seeded to evaluate their characteristics in vitro and in a critical sized defect in Sprague-Dawley rats in vivo. It was shown that the 1% MWCNT group was the most suitable for bone tissue engineering. Our results demonstrated that scaffolds loaded with BMP-9 promoted differentiation of BMMSCs into osteoblasts in vitro and induced more bone formation in vivo. To conclude, nHA/ColI/MWCNT scaffolds loaded with BMP-9 possess high biocompatibility and osteogenesis and are a good candidate for use in bone tissue engineering.

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The effect of extracellular calcium and inorganic phosphate on the growth and osteogenic differentiation of mesenchymal stem cells in vitro : implication for bone tissue engineering

Biomedical Materials ◽

10.1088/1748-6041/4/2/025004 ◽

2009 ◽

Vol 4 (2) ◽

pp. 025004 ◽

Cited By ~ 81

Author(s):

Yu Kan Liu ◽

Qiao Zhi Lu ◽

Rui Pei ◽

Hui Jiao Ji ◽

Guo Shun Zhou ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Tissue Engineering ◽

Osteogenic Differentiation ◽

Bone Tissue ◽

Inorganic Phosphate ◽

Extracellular Calcium

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In vitro evaluation of novel low-pressure spark plasma sintered HA–BG composite scaffolds for bone tissue engineering

RSC Advances ◽

10.1039/d0ra04227g ◽

2020 ◽

Vol 10 (40) ◽

pp. 23813-23828

Author(s):

Muhammad Rizwan ◽

Krishnamurithy Genasan ◽

Malliga Raman Murali ◽

Hanumantha Rao Balaji Raghavendran ◽

Rodianah Alias ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Osteogenic Differentiation ◽

Bone Tissue ◽

Composite Scaffold ◽

Composite Scaffolds ◽

In Vitro Evaluation ◽

Monocyte Migration ◽

Spark Plasma

HB 30 S composite scaffold inhibits Staphylococcus spp., supports the biocompatibility and osteogenic differentiation of hBMSCs and resists monocyte migration.

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Dextran-coated fluorapatite nanorods doped with lanthanides in labelling and directing osteogenic differentiation of bone marrow mesenchymal stem cells

Journal of Materials Chemistry B ◽

10.1039/c4tb00303a ◽

2014 ◽

Vol 2 (23) ◽

pp. 3609-3617 ◽

Cited By ~ 11

Author(s):

Haifeng Zeng ◽

Xiyu Li ◽

Fang Xie ◽

Li Teng ◽

Haifeng Chen

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Bone Tissue Engineering ◽

Osteogenic Differentiation ◽

Bone Tissue ◽

Novel Approach ◽

Marrow Mesenchymal Stem Cells

A novel approach for labelling and tracking BMSCs in bone tissue engineering by using dextran-coated fluorapatite nanorods doped with lanthanides.

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Gelatin/Hydroxyapatite Scaffolds: Studies on Adhesion, Growth and Differentiation of Mesenchymal Stem Cells

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.93-94.121 ◽

2010 ◽

Vol 93-94 ◽

pp. 121-124

Author(s):

Nuttapon Vachiraroj ◽

Siriporn Damrongsakkul ◽

Sorada Kanokpanont

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Calcium Content ◽

Population Doubling ◽

Population Doubling Time ◽

3 Dimensional

In this work, we developed a 3-dimensional bone tissue engineering scaffold from type B gelatin and hydroxyapatite. Two types of scaffolds, pure gelatin (pI~5) (Gel) and gelatin/hydroxyapatite (30/70 wt./wt.) (Gel/HA), were prepared from concentrated solutions (5% wt./wt.) using foaming/freeze drying method. The results SEM revealed the interconnected-homogeneous pores of Gel and Gel/HA were 121  119 and 148  83m, respectively. Hydroxyapatite improved mechanical property of the gelatin scaffolds, especially at dry state. Compressive modulus of Gel and Gel/HA scaffolds were at 118±21.68 and 510±109.08 kPa, respectively. The results on in vitro cells culture showed that Gel/HA scaffolds promoted attachment of rat’s mesenchymal stem cells (MSC) to a 1.23 folds higher than the Gel scaffolds. Population doubling time (PDT) of MSC on Gel and Gel/HA scaffolds were 51.16 and 54.89 hours, respectively. In term of osteogenic differentiation, Gel/HA scaffolds tended to enhance ALP activity and calcium content of MSC better than those of the Gel scaffold. Therefore the Gel/HA scaffolds had a potential to be applied in bone tissue engineering.

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Zein/gelatin/nanohydroxyapatite nanofibrous scaffolds are biocompatible and promote osteogenic differentiation of human periodontal ligament stem cells

Biomaterials Science ◽

10.1039/c8bm01653d ◽

2019 ◽

Vol 7 (5) ◽

pp. 1973-1983 ◽

Cited By ~ 13

Author(s):

Qianmin Ou ◽

Yingling Miao ◽

Fanqiao Yang ◽

Xuefeng Lin ◽

Li-Ming Zhang ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Bone Tissue Engineering ◽

Osteogenic Differentiation ◽

Bone Tissue ◽

Tissue Regeneration ◽

Periodontal Ligament ◽

Periodontal Ligament Stem Cells ◽

Nanofibrous Scaffolds

In bone tissue engineering, it is important for biomaterials to promote the osteogenic differentiation of stem cells to achieve tissue regeneration.

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Osteogenic potential of mesenchymal stem cells from rat mandible to regenerate critical sized calvarial defect

Journal of Tissue Engineering ◽

10.1177/2041731419830427 ◽

2019 ◽

Vol 10 ◽

pp. 204173141983042 ◽

Cited By ~ 9

Author(s):

Dong Joon Lee ◽

Jane Kwon ◽

Luke Current ◽

Kun Yoon ◽

Rahim Zalal ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Osteogenic Potential ◽

Embryonic Origin ◽

Rat Mandible

Although bone marrow–derived mesenchymal stem cells (MSCs) have been extensively explored in bone tissue engineering, only few studies using mesenchymal stem cells from mandible (M-MSCs) have been reported. However, mesenchymal stem cells from mandible have the potential to be as effective as femur-derived mesenchymal stem cells (F-MSCs) in regenerating bone, especially in the orofacial regions, which share embryonic origin, proximity, and accessibility. M-MSCs were isolated and characterized using mesenchymal stem cell–specific markers, colony forming assay, and multi-potential differentiation. In vitro osteogenic potential, including proliferation, osteogenic gene expression, alkaline phosphatase activity, and mineralization, was examined and compared. Furthermore, in vivo bone formations of F-MSCs and M-MSCs in rat critical sized defect were evaluated using microCT and histology. M-MSCs from rat could be successfully isolated and expanded while preserving their MSC’s characteristics. M-MSCs demonstrated a comparable proliferation and mineralization potentials and in vivo bone formation as F-MSCs. M-MSCs is a promising cell source candidate for craniofacial bone tissue engineering.

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Three-dimensional Printed Polylactic Acid and Hydroxyapatite Composite Scaffold with Urine-derived Stem Cells Treatment for Bone Defects

10.21203/rs.3.rs-996295/v1 ◽

2021 ◽

Author(s):

Xiang Zhang ◽

Jialei Chen ◽

Hongren Wang ◽

Xin Duan ◽

Feng Gao

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Polylactic Acid ◽

Composite Scaffold ◽

Bone Defects ◽

Skull Defect ◽

Defect Area ◽

3D Printed

Abstract BACKGROUND: Bone defects still pose various challenges in osteology. As one of the treatment options for bone defects, bone tissue engineering requires biomaterials with good biocompatibility and seed cells with good differentiation capacity. This study aimed to fabricate a 3D-printed polylactic acid and hydroxyapatite (PLA/HA) composite scaffold with urine-derived stem cells (USCs) to study its therapeutic effect in a model of skull defect in rats.METHODS: USCs, isolated and extracted from the urine of healthy adult males, were inoculated onto a 3D-printed PLA/HA composite scaffold and a PLA scaffold. Skull defect model rats were randomly divided into three groups (control, PLA, and PLA/HA). Twelve weeks after implanting scaffolds containing USCs into rats with a skull defect, the therapeutic efficacy was evaluated by real-time PCR, micro-CT, histology, and immunohistochemistry.RESULTS: The 3D-printed PLA/HA composite scaffold had good mechanical properties and porosity. The adhesion and proliferation of USCs on scaffolds also demonstrated excellent biocompatibility. PLA and PLA/HA containing USCs promoted bone regeneration in the defect area, supported by the general observation and CT images at 12 weeks after treatment, with coverage of 74.6%±1.9% and 96.7%±1.6%, respectively. Immunohistochemical staining showed a progressive process of new bone formation on PLA/HA scaffolds containing USCs at the defect site compared to that in PLA and control groups.CONCLUSION: The 3D-printed PLA/HA composite scaffold with USCs was successfully applied to the skull defect in rats. Under the linkage of the scaffold, the proliferation, differentiation, and osteogenesis expression of USCs were promoted near the bone defect area. These findings demonstrated broad application prospects of PLA/HA scaffolds with USCs in bone tissue engineering.

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