Values of urinalysis dipstick in evaluating high-protein, low-carbohydrate, low-fat diets in male wistar rats

Obesity is one of the diseases caused by unhealthy eating patterns and diets. An unhealthy diet can increase cholesterol levels in the blood which will then accumulate on the inner walls of blood vessels, causing atherosclerosis. This study aims to determine the effect of long-term high-protein, low-carbohydrate and low-fat diet on heart function by examining male CKMB & Troponin T levels of Rattus Norvegicus. This research was conducted experimentally in a laboratory with a Post-Test Control Design Group research design. The number of samples was 20 male wistar rats and divided into two groups (standard feed group) and the TPRKRL diet (high protein, low carbohydrate, low fat diet group). Blood was examined in the Hasanuddin University medical research center laboratory using the ELIZA method. The analysis used to determine the difference between the two groups was the Maan-Whitney test. The results showed that the macronutrient composition of the high-protein diet had significantly higher protein content and percentage and lower carbohydrates (protein 78.25% vs. 15.25%; CHO 10.5% vs. 52.2%) compared to the standard feed composition. Meanwhile, post-intervention blood and organ Troponin T levels in the treatment group were 25.45 ± 1.80 and 1022.52 ± 447.89 pg/ml, respectively. And post-intervention blood and organ Troponin T levels in the control group were 23.99 ± 1.24 and 1117.56 ± 324.44 pg/ml. The TPRLLK diet was able to significantly reduce body weight compared to the standard diet and the TPRLLK diet did not increase the enzyme markers of heart damage.

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Effects of proportional high-protein/low-carbohydrate formulated diet consumption in diabetic rats: Beneficial impact on glycemic and weight control

African Journal of Food, Agriculture, Nutrition and Development ◽

10.18697/ajfand.95.19950 ◽

2020 ◽

Vol 20 (07) ◽

pp. 16984-16996

Author(s):

MMC Anyakudo ◽

◽

DO Adeniji ◽

Keyword(s):

Body Weight ◽

Wistar Rats ◽

Treated Group ◽

Morphological Features ◽

High Protein ◽

Control Group ◽

Formulated Diet ◽

Low Carbohydrate ◽

Male Wistar Rats ◽

And Control

The metabolic response to nutrient ingestion and the rate of digestion and absorption of nutrient molecules in bowel physiology plays an important role in the metabolic control of some human chronic non-infectious diseases. This experimentally-controlled designed nutritional study which lasted eight weeks aimed to determine the effects of proportional high-protein/low-carbohydrate (HP/LC) formulated diet on glycemic tolerance, glycemic control, body weight, organ weight and organ morphometry in healthy and diabetic adult male Wistar rats. Twenty-four male Wistar rats purchased from a disease-free stock were randomly categorized into four groups (n = 6, each) after two weeks acclimatization period in raised stainless steel cages with 6 mm2mesh floor and replaceable numbered blotters papers placed under each cage in a well-ventilated animal house. Animal groups include: Healthy control group (HC), Healthy treated group (HT), Diabetic control group (DC) and Diabetic treated group (DT. The animals were fed according to the experimental design with water ad libitumfor eight weeks. Diabetes was inducted with freshly prepared alloxan monohydrate solution (150 mg/kg bw, intraperitoneally). Body weights and fasting blood sugar concentrations were measured twice weekly, while oral glucose tolerance test was conducted on the last day of the eighth-week study and subsequently followed by organs extraction after anesthesia for weight and gross assessment. Proportional high-protein/low-carbohydrate formulated diet caused significant reduction in mean body weight of treated diabetic (DT: 22.6%; P= .001) and healthy (HT: 5.8%; P= .007) rats while the control animals on control diet recorded significant (P< .05) increase in body weight gain (DC: 12.4%; HC: 11.2%). Glycemic tolerance and control improved significantly in diabetic treated rats over that of the healthy treated rats. Gross morphometry of the extracted organs (kidneys, liver, heart, lungs, spleen and testes) revealed sustained normal morphological features without any visible lesion. In conclusion, consumption of proportional high-protein/low-carbohydrate formulated diet enhanced body weight reduction and sustained normal organ morphological features with good glycemic tolerance and control in experimental rats, suggesting its dietary potentiality, safety and suitability to ameliorate obesity-related diabetes.

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