scholarly journals Effects of proportional high-protein/low-carbohydrate formulated diet consumption in diabetic rats: Beneficial impact on glycemic and weight control

2020 ◽  
Vol 20 (07) ◽  
pp. 16984-16996
Author(s):  
MMC Anyakudo ◽  
◽  
DO Adeniji ◽  
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Treated Group ◽  
Morphological Features ◽  
High Protein ◽  
Control Group ◽  
Formulated Diet ◽  
Low Carbohydrate ◽  
Male Wistar Rats ◽  
And Control

The metabolic response to nutrient ingestion and the rate of digestion and absorption of nutrient molecules in bowel physiology plays an important role in the metabolic control of some human chronic non-infectious diseases. This experimentally-controlled designed nutritional study which lasted eight weeks aimed to determine the effects of proportional high-protein/low-carbohydrate (HP/LC) formulated diet on glycemic tolerance, glycemic control, body weight, organ weight and organ morphometry in healthy and diabetic adult male Wistar rats. Twenty-four male Wistar rats purchased from a disease-free stock were randomly categorized into four groups (n = 6, each) after two weeks acclimatization period in raised stainless steel cages with 6 mm2mesh floor and replaceable numbered blotters papers placed under each cage in a well-ventilated animal house. Animal groups include: Healthy control group (HC), Healthy treated group (HT), Diabetic control group (DC) and Diabetic treated group (DT. The animals were fed according to the experimental design with water ad libitumfor eight weeks. Diabetes was inducted with freshly prepared alloxan monohydrate solution (150 mg/kg bw, intraperitoneally). Body weights and fasting blood sugar concentrations were measured twice weekly, while oral glucose tolerance test was conducted on the last day of the eighth-week study and subsequently followed by organs extraction after anesthesia for weight and gross assessment. Proportional high-protein/low-carbohydrate formulated diet caused significant reduction in mean body weight of treated diabetic (DT: 22.6%; P= .001) and healthy (HT: 5.8%; P= .007) rats while the control animals on control diet recorded significant (P< .05) increase in body weight gain (DC: 12.4%; HC: 11.2%). Glycemic tolerance and control improved significantly in diabetic treated rats over that of the healthy treated rats. Gross morphometry of the extracted organs (kidneys, liver, heart, lungs, spleen and testes) revealed sustained normal morphological features without any visible lesion. In conclusion, consumption of proportional high-protein/low-carbohydrate formulated diet enhanced body weight reduction and sustained normal organ morphological features with good glycemic tolerance and control in experimental rats, suggesting its dietary potentiality, safety and suitability to ameliorate obesity-related diabetes.

scholarly journals Melatonin menurunkan berat badan tetapi tidak menurunkan kadar TNF-α pada tikus wistar jantan yang diberi minyak jelantah selama 28 hari

2017 ◽  
Vol 5 (2) ◽  
pp. 127-132
Author(s):  
Anugrah Novianti ◽  
Edi Dharmana ◽  
Nyoman Suci Widyastiti
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Treated Group ◽  
Waste Cooking Oil ◽  
The Body ◽  
Control Group ◽  
Cooking Oil ◽  
Tnf Α ◽  
Significant Difference ◽  
Male Wistar Rats

Backgound: Non Alcoholic Fatty Liver Disease (NAFLD) occurs when the intake and free fatty acid synthesis occurs more frequently than its oxidation and resecretion in the blood. Melatonin is a powerful antioxidant that can boost the synthesis of endogenous antioxidants in the body, suppress the inflammatory response and inhibit the formation of steatosis.Objective: To analyze the effect of melatonin supplementation in reducing body weight andTNF-α levels in male Wistar rats were fed by waste cooking oil.Methods: True experimental studyusing post-test only control group design. This study was done on 18 male wistar rats were divided into 3 groups : the positive control group (P0) was administrated waste cooking oil, the treated group 1 (P1) was administrated waste cooking oil and 5mg/kgBW melatonin, and the treated group 2 (P2) was administrated waste cooking oil and 10mg/kgBW melatonin for 28 days.Data analysis using One Way ANOVA test and followed by Tukey test to determine the most effective dose of melatonin.Results: There was significant difference in body weightbetween P2group and K0 group (p=0,019) with the mean body weight difference was 19,167g lower than K0 group. There was no difference in TNF-α levels between the three groups (p=0,155). Conclusion: Melatonin dose of 10mg/kgBWloses body weight male Wistar rats have given by waste cooking oil for 28 days, but does not reduce TNF-α levels.

scholarly journals Effect of Pistacia atlantica subsp. kurdica Gum in Experimental Periodontitis Induced in Wistar Rats by Utilization of Osteoclastogenic Bone Markers

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5819
Author(s):  
Shokhan H. Azeez ◽  
Shanaz M. Gaphor ◽  
Aram M. Sha ◽  
Balkees T. Garib
Keyword(s):  
Wistar Rats ◽  
Bone Markers ◽  
Inflammatory Cells ◽  
Treated Group ◽  
Surrounding Tissue ◽  
Control Group ◽  
Pistacia Atlantica ◽  
Male Wistar Rats ◽  
Experimental Periodontitis ◽  
Serum Rankl

The aim of this study was to assess the effect of local application of essential oil of Pistacia atlantica kurdica (EOK) gel in treatment of experimentally induced periodontitis in rats and its effect on osteoclastogenic bone markers. Twenty-four male Wistar rats of 250 to 350 g were used in this study and were allocated into four groups. Control negative (without induced periodontitis), control positive (induced experimental periodontitis left without treatment), treatment control (induced experimental periodontitis and treated with Chlorhexidine gel) and EOK treated group (induced experimental periodontitis treated with EOK gel). The animals were sacrificed after 30 days, and the mandibular central incisor and surrounding tissue were dissected from the mandible and further processed for preparing H&E slides. Inflammatory cells, osteoclast cells, and periodontal ligament (PDL) were examined and measured histologically. Finally, the mean concentrations of both markers, receptor activator of nuclear factor kappa-Β ligand (RANKL) and (Interleukin-1β) IL-1β, were analyzed by ELISA. A significant reduction of inflammatory reaction and osteoclast numbers with improvement of PDL and low mean concentrations of RANKL and IL-1β were seen in the EOK treated group in comparison to the control group and the chlorhexidine group as well. The extract showed a protective effect in the healing of periodontitis that had been induced in rats and decreased bone resorption by down regulation of serum RANKL and IL-1β markers.

scholarly journals The Green Bitter Weed (Hymenoxys odorato) Plant Extract Is Toxic to the Liver and Kidney of Wistar Rats

2018 ◽  
Author(s):  
John Juma Ochieng ◽  
Isaac Echoru ◽  
Musa Ajibola Iyiola
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Complete Blood Count ◽  
Medical Personnel ◽  
Control Group ◽  
Dependent Manner ◽  
Snake Bites ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
High Doses

Background: Medicinal plants are of great importance to health of individual and communities. About 80% of the population in Uganda relies on traditional medicine because western-trained medical personnel are limited especially in villages. Most Ugandans use Hymenoxys odorato for medicinal purposes e.g. to treat colds, fever, coughs, anti-helminthes, locally used as tea, anti-allergy and also as an anti-venom to relieve snake bites. Method: A group of 25 male wistar rats of 150 g&ndash;210 g were kept for 14 days while being fed and treated with the extract. At 14th day, anesthesia was given and blood samples collected by cardiac puncture for hematological and biochemical investigations. Serum was analyzed for Alkaline Phosphatase, Aspartate Transaminase and Alanine Transaminase while whole blood was used for complete blood count. The liver and kidney were removed and placed in 10% formalin to prepare for histology staining using haematoxylin and eosin technique. Results: The extract elevated hepatic biomarker enzymes i.e. ALP, ALT and AST. The increase was found to be significantly different (P &gt; 0.05) at 400 and 500 mg/kg doses as compared to the control group. Histological sections of the liver showed distortion of liver cytoarchitecture, steatosis, necrosis of hepatocytes and congestion of the sinusoids at high doses 300, 400 and 500 mg/kg body weight. In the sections of the kidney, there was mild distortion of the integrity of the kidney with glomerular hypercellularity at high doses (400 and 500 mg/kg per body weight). Conclusion: Hymenoxys odorato aqueous extract has toxic effects on the liver and kidney of wistar rats. The effects were observed to be in a dose dependent manner.

scholarly journals PENGARUH PEMBERIAN SAGU DIBANDING NASI TERHADAP BERAT BADAN TIKUS WISTAR

e-CliniC ◽  
2013 ◽  
Vol 3 (2) ◽  
Author(s):  
Eny A. Watumlawar ◽  
Sarah M. Warouw ◽  
Stefanus Gunawan
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Wilcoxon Test ◽  
Control Group ◽  
Increase Body Weight ◽  
Control Group Design ◽  
Tissue Mass ◽  
Group Design ◽  
Post Test ◽  
Male Wistar Rats

Abstract: Weight is one measure that gives description of the tissue mass including body fluids. Weight change is nfluenced by nutriment consumption. Sago contains high carbohydrates that can increase body weight. This study aimed to obtain the effect of sago compared to rice to body weight of wistar rats. This was a random laboratory experimental pre-post test with control group design. Subjects were male wistar rats, aged 5-6 months. The rats were divided in two groups: rice group as control and sago group. Sago was cooked in papeda form as much as 75 g of dried sago and 300 mL water. The rats were fed for 2 weeks. Data were analyzed by using the Wilcoxon test. The results showed that the sago group showed an increase of body weight significantly (p=0,001) meanwhile the rice group lose body weight significantly (p=0,001). Conclusion: Sago can increase body weight of wistar rats significantly.Keywords: body weight, sago, riceAbstrak: Berat badan merupakan salah satu ukuran yang memberikan gambaran massa jaringan termasuk cairan tubuh. Salah satu yang memengaruhi berat badan yaitu dengan mengonsumsi makanan bergizi. Sagu memiliki kandungan karbohidrat (pati) yang besar dan dapat meningkatkan berat badan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian sagu terhadap berat badan dibanding nasi pada tikus wistar. Desain penelitian ini ialah eksperimental laboratorium acak pre-post test with control group design. Subjek penelitian ialah tikus wistar jantan berusia 5-6 bulan dibagi atas 2 kelompok: kelompok nasi (kontrol) dan kelompok sagu. Sagu dimasak dalam bentuk papeda sebanyak 75 g sagu kering dan 300 mL air. Tikus diperlihara selama 2 minggu. Berat badan diukur dengan menggunakan timbangan. Data dianalisis dengan uji Wilcoxon. Hasil penelitian menunjukkan bahwa pemberian sagu sebanyak 2 cc setiap hari selama seminggu mampu meningkatkan berat badan secara bermakna (p = 0,001) sedangkan pemberian nasi menurunkan berat badan (p=0,001). Simpulan: Pemberian sagu dapat meningkatkan berat badan tikus wistar secara bermakna.Kata kunci: berat badan, sagu, nasi

The Effect of Long-Term High Protein, Low Carbohydrate, and Low Fat Diet in CKMB Enzymes and Troponin T in Male Wistar Rats

2021 ◽  
Vol 3 (2) ◽  
pp. 58-64
Author(s):  
Asma Amaliah Idrus ◽  
Aryadi Arsyad ◽  
Aminuddin
Keyword(s):  
Wistar Rats ◽  
Troponin T ◽  
High Protein ◽  
Low Fat ◽  
Post Intervention ◽  
Feed Composition ◽  
Low Fat Diet ◽  
Low Carbohydrate ◽  
Male Wistar Rats

Obesity is one of the diseases caused by unhealthy eating patterns and diets. An unhealthy diet can increase cholesterol levels in the blood which will then accumulate on the inner walls of blood vessels, causing atherosclerosis. This study aims to determine the effect of long-term high-protein, low-carbohydrate and low-fat diet on heart function by examining male CKMB & Troponin T levels of Rattus Norvegicus. This research was conducted experimentally in a laboratory with a Post-Test Control Design Group research design. The number of samples was 20 male wistar rats and divided into two groups (standard feed group) and the TPRKRL diet (high protein, low carbohydrate, low fat diet group). Blood was examined in the Hasanuddin University medical research center laboratory using the ELIZA method. The analysis used to determine the difference between the two groups was the Maan-Whitney test. The results showed that the macronutrient composition of the high-protein diet had significantly higher protein content and percentage and lower carbohydrates (protein 78.25% vs. 15.25%; CHO 10.5% vs. 52.2%) compared to the standard feed composition. Meanwhile, post-intervention blood and organ Troponin T levels in the treatment group were 25.45 ± 1.80 and 1022.52 ± 447.89 pg/ml, respectively. And post-intervention blood and organ Troponin T levels in the control group were 23.99 ± 1.24 and 1117.56 ± 324.44 pg/ml. The TPRLLK diet was able to significantly reduce body weight compared to the standard diet and the TPRLLK diet did not increase the enzyme markers of heart damage.

The Effects of Food Deprivation of Rats On Swimming To Exhaustion

Behaviour ◽  
1966 ◽  
Vol 26 (3-4) ◽  
pp. 351-356 ◽  
Author(s):  
Edward T. Uyeno ◽  
R. Alan Graham
Keyword(s):  
Body Weight ◽  
Food Deprivation ◽  
Wistar Rats ◽  
Control Group ◽  
Dorsal Skin ◽  
The Third ◽  
Male Wistar Rats ◽  
Ad Libitum ◽  
Fourth Experiment ◽  
Ad Libitum Feeding

AbstractMale Wistar rats were deprived of food for 3 days and then forced to swim continuously to a criterion of exhaustion. Controls swam after ad libitum feeding. In the first experiment a load equal to 11% of the rat's body weight was attached to the dorsal skin near the tail during the swim. In a second study, two matched groups, treated identically as those in the first study swam without a load in water treated with a wetting agent, "Aerosol O.T." In a third experiment, controls as well as experimentals were deprived of food for three days. The control group, however, was fed for 30 minutes, immediately before the swim. A fourth experiment was conducted in a similar manner as the third, except both groups were deprived 6 hours. In each of the experiments the deprived groups swam significantly longer than the fed groups.

scholarly journals Attenuation of Carbon Tetrachloride – Induced Hepatoxicity by Dacryodes edulis Seeds Ethanolic Extract in Male Wistar Rats

2020 ◽  
Vol 11 (2) ◽  
pp. 9490-9500
Keyword(s):  
Body Weight ◽  
Carbon Tetrachloride ◽  
Wistar Rats ◽  
Corn Oil ◽  
Ethanolic Extract ◽  
Seed Extract ◽  
Control Group ◽  
Once Daily ◽  
Male Wistar Rats ◽  
Dacryodes Edulis

Dacryodes edulis (African pear) seed has a long history of use in ethnomedicine. However, there is a paucity of information on its ameliorative potential on hepatic damage. In the present study, the potentials of D. edulis ethanolic seed extract (DET) in attenuating carbon tetrachloride (CCl4)-induced hepatotoxicity in male Wistar rats was assessed. Ninety male Wistar rats were divided into 3 groups (n = 10) designated: control, CCl4, and DET, respectively. Animals in the control group were administered 2 mL/kg body weight corn oil, while those assigned to CCl4 and DET groups were administered i.p. 30% CCl4 in corn oil (1:1 v/v) at 2 mL/kg. Treatment was administered once every 24 h consecutively for 72 h. Thereafter, control and CCl4 groups were treated once daily with 1 mL distilled water by gavage, while DET rats were treated once daily with 1500 mg/kg DET by gavage. At every 7 days interval and for 28 consecutive days, animals were starved overnight, weighed, and six rats from each group were sacrificed under anesthesia to collect blood and liver samples. After 28 days, remarkable improvements in feed intake, body weight, serum levels of hepatic function indicators, and hepatic histology were observed in DET rats compared to CCl4 rats. These results suggest a possible ameliorative potential of D. edulis ethanolic seed extract against CCl4-induced hepatic injury.

scholarly journals Effects of Oral Administration of Monosodium Glutamate (MSG) on Obesity in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 5 (9) ◽  
pp. 879-882
Author(s):  
Mulyati Sri Rahayu ◽  
Sri Wahyuni ◽  
Yuziani
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Body Weight Gain ◽  
Monosodium Glutamate ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Group 2

Introduction: Monosodium glutamate (MSG) is one of the most widely employed food enhancers. Although the umami compound, controversy persists regarding the effects of MSG intake on body weight. Chronic MSG intake may result in excessive body weight gain and obesity. Consumption of MSG result in organ damage, cardiovascular disease, oxidative stress, and also risk factors for obesity. This study aims to determine the effect of oral MSG on obesity in adult male Wistar rats (Rattus norvegicus).Methods: This true experimental study used the post-test control group design. Twenty-four adult male Wistar rats were randomly divided into four groups: control (received distilled water), Group 1 (MSG 0.378 mg/gr BW), Group 2 (0.756 mg/gr BW) and Group 3 (1.512 mg/gr BW). The obesity parameter was obtained by the Lee index. Kruskal-Wallis test follows by Mann-Whitney test were used to compare the Lee index between groups.Results: Lee’s index mean for each group was 358.4%, 314.1%, 287.8%, and 320.9%, respectively. The Kruskal Wallis test showed a significant difference in the Lee index between groups (p = 0.043). A follow-up test using Mann-Whitney found a significant difference between group 2 and the control group (p = 0.043, p <0.05). The mean of Lee index of group 2 was 70.51% lower than the control group.Conclusion: This study concluded that Lee index was not increased in MSG-treated rats than in the control group after oral MSG intervention for 21 days.

scholarly journals Preliminary Study on the Effect of Methanolic Extract of Watermelon (Citrullus lanatus) Rind on Prednisolone Suppressed Immunity in Male Wistar Rats

2021 ◽  
pp. 1-6
Author(s):  
Nyejirime Young Wike ◽  
Mobisson Samuel Kelechi ◽  
Godspower Onyeso ◽  
Okekem Amadi ◽  
Elizabeth Eepho Krukru
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Citrullus Lanatus ◽  
Control Group ◽  
Group 4 ◽  
Watermelon Rind ◽  
Male Wistar Rats ◽  
Group 3 ◽  
Group 2

Citrullus lanatus thumb (Cucurbitaceae) commonly called watermelon is widely consumed in this part of the world as food and medicine. This study was carried out to examine the effect of methanolic extract of watermelon (Citrullus lanatus) rind on prednisolone suppressed immunity in male wistar rats. A total of 20 male wistar rats weighing 150-294g were used in 4 groups with five rats each. Group 1, the control group was given distilled water and feed, Group 2 was given 200 mg/kg body weight of methanolic extract of watermelon rind, Group 3 rats were given 2.5 mg/kg body weight of prednisolone and Group 4 rats were given 2.5 mg/kg body weight of prednisolone and 200 mg/kg body weight of methanolic extract of watermelon rind. Prednisolone and the methanolic extract of watermelon rind were administered orally for a period of 30 days. Blood samples were collected by cardio puncture from the rats for white blood cell (WBC), lymphocyte, and granulocyte and monocyte counts at the end of the experiment.  The data were statistically analysed using one-way ANOVA (Analysis of variance). Data were considered significant at p<0.05. The results obtained showed that methanolic extract of watermelon rind caused a significant increasen in immune function of rats when compared with the control and immune suppressed rats.

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