scholarly journals Genetic diversity and differentiation of cytochrome oxidase subunit I (COI) gene of Rasbora lateristriata bleeker in different habitat

2021 ◽  
Vol 1098 (5) ◽  
pp. 052043
Author(s):  
S A M P Suryani ◽  
I G P Wirawan ◽  
R Dwiyani ◽  
M Sritamin
2019 ◽  
Vol 12 (1) ◽  
Author(s):  
NUR ZAWANI MUSTAFA KAMAL ◽  
MUHAMMAD SIRI DANGNGA ◽  
IRMAYANI ◽  
Darlina Md. Naim

Abstract. Kamal MZM, Dangnga MS, Irmayani, Md. Naim D. 2020. Species identification and genetic diversity of Aedes in Penang (Malaysia) based on Cytochrome Oxidase Subunit I. Nusantara Bioscience 12: 6-12. Cases of dengue fever are expanding globally and recently it was identified as being notable in remote/rural regions in South-East Asia in spite of the previous belief that it is outbreak to urban areas, especially in Malaysia. In an effort to constraint, the extent of the virus, the most important thing is to know the distribution of the vector that causes the disease. This research aims to recognize all species of mosquitoes distributed in Penang utilizing DNA barcode method in which the mtDNA cytochrome oxidase subunit 1 (COI) as a gene marker. Approximately 497 larvae of mosquitoes were obtained from four regions in Penang, Malaysia namely Central Seberang Perai, North Seberang Perai, North East, and South West of Penang. All samples were extracted and PCR amplified. Sequences were employed in BLAST of GenBank and aligned with MUSCLE. The ABGD analysis was performed to partition all the samples and substitution saturation analysis was employed in DAMBE. Phylogenetic relationships among nucleotides were reconstructed utilizing Maximum Likelihood (ML) and Neighbor-Joining (NJ) calculated following Kimura 2-parameter. Intra and interspecific genetic variation among population were also conducted based on Tamura-Nei parameter and all analyses were employed in MEGA version 6.0. As a result, COI gene has successfully recognized all larvae obtained in this study. We found a total of six species (including outgroup sequence) as revealed by BLASTn, ABGD and phylogenetic analysis. Genetic distance test has further supported the results. This study revealed that there is no visible population structure and all species have a uniform distribution throughout all sampling locations in Penang. We also found that Ae. albopictus has occupied most areas in Penang, and this includes urban locations, hence, suggest that Ae. albopictus probably succeed Ae. aegypti as the main vector for dengue virus in Penang.


ENTOMON ◽  
2019 ◽  
Vol 44 (2) ◽  
pp. 155-160
Author(s):  
Keerthy Vijayan ◽  
R. Sugantha Sakthivel ◽  
T.V. Sajeev

The presence of the body colour polymorphism in the tropical invasive pest giant African snail is reported for the first time from South India. Three different body colour polymorphs were recognised viz. grey, black and white. The grey body colour is the most common polymorph. The black and white colour polymorphs are found to be in almost equal proportions in the reported localities with the grey counterparts. The cytochrome oxidase subunit I (COI) sequences of the three colour polymorphs are found to be identical. The presence of the body colour polymorphism in south India may be attributed to the avian predation and other selection pressures.


Meta Gene ◽  
2020 ◽  
Vol 25 ◽  
pp. 100759
Author(s):  
Yesenia Margarita Vega-Sánchez ◽  
María Olalla Lorenzo-Carballa ◽  
Diogo Silva Vilela ◽  
Rhainer Guillermo-Ferreira ◽  
Ricardo Koroiva

2017 ◽  
Vol 22 (2) ◽  
pp. 67-74
Author(s):  
Muhammad Nur Findra ◽  
Isdradjad Setyobudiandi ◽  
Nurlisa Alias Butet ◽  
Dedy Duryadi Solihin

Giant clam population has been decreased in a few years. Resource management requires information from various aspects, such as ecological, population, and other aspects. This study was aimed at assessing the genetic profile of Tridacna giant clam in Wakatobi National Park waters using Cytochrome oxidase subunit I (COI) genetic marker. Sample collection was conducted around the three main islands, i.e., Wangi-wangi, Kaledupa, and Tomia. Genetic analysis using COI gene may contribute in identifying giant clams up to the species level and showed the relationship among species. The research found 41 specific nucleotide sites for the clams. T. crocea, T. squamosa and T. maxima had 2, 15 and 24 sites, respectively. COI gene as a biological marker was able to separate groups of giant clam by species. Nucleotide variation of T. crocea from Wakatobi was the highest among other locations, so it could be used as a genetic source for translocation and domestication. Keywords: cytochrome oxidase subunit I, specific nucleotide, Tridacna, Wakatobi National Park


2020 ◽  
Vol 4 (1) ◽  
pp. 8-16
Author(s):  
Dian Rezki Muliani ◽  
Fredinan Yulianda ◽  
Nurlisa A Butet

Oysters belong to the Crassostrea, which is a type of shellfish that lives as benthos in waters under the same substrate and environmental conditions will exhibit similar morphological changes in response. There are many types of oysters that have a similar shape are often an obstacle to differentiate of Crassostrea species through morphological identification. The purpose of this research was to identify the types of oysters found in the waters of Delta Cimanuk through analysis of the morphology and nucleotide diversity of Cytochrome Oxidase subunit I (COI) gene, as basic information on proper management and conservation. The morphological identification results show that there were two species of the Crassostrea. Identification of species through the Barcoding DNA technique shows that there is one type of oyster: Crassostrea iredalei with an accuracy of 99.5%.


2017 ◽  
Vol 44 (2) ◽  
pp. 175-184
Author(s):  
Mehnus Tabassum ◽  
Hawa Jahan ◽  
Gulshan Ara Latifa

DNA barcoding has been proposed as a means of quick species identification using a short standardized segment of DNA. Two species (Eleotris fusca and Glossogobius giuris) from the family Gobiidae and Eleotridae were selected for DNA barcoding using samples collected from different regions of Bangladesh. Cytochrome Oxidase Subunit I (COI) gene was sequenced from two different gobi fishes and compared with two previously published similar sequences from the genera Eleotris and Glossogobius. Multiple sequence alignment and the molecular systematic study were performed. The DNA barcode technique identified the two species. The study provides a good example of how DNA barcoding can build upon its primary mission of species identification and use available data to integrate genetic variation investigated at the local scale into a global framework.Bangladesh J. Zool. 44(2): 175-184, 2016


Meta Gene ◽  
2020 ◽  
Vol 25 ◽  
pp. 100739 ◽  
Author(s):  
Saif Ul Islam ◽  
Muhammad Arif ◽  
Wenzhong Lin ◽  
Waqar Islam ◽  
Muhammad Qasim ◽  
...  

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