scholarly journals HPLC Method for Simultaneous Determination of Dextromethorphan Hydrobromide, Chlorpheniramine Maleate and Potassium Sorbate in Cough Syrup

2021 ◽  
Vol 1115 (1) ◽  
pp. 012035
Author(s):  
T Yuliana ◽  
S S N Gustin ◽  
A Alamsyah ◽  
S Budiman ◽  
A Hardian ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Hplc Method ◽  
Potassium Sorbate ◽  
Chlorpheniramine Maleate ◽  
Cough Syrup ◽  
Dextromethorphan Hydrobromide

scholarly journals Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft Drinks

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Bürge Aşçı ◽  
Şule Dinç Zor ◽  
Özlem Aksu Dönmez
Keyword(s):  
Simultaneous Determination ◽  
Flow Rate ◽  
Mobile Phase ◽  
High Performance ◽  
Food Additives ◽  
Hplc Method ◽  
Soft Drinks ◽  
Phase Ratio ◽  
Potassium Sorbate

Box-Behnken design was applied to optimize high performance liquid chromatography (HPLC) conditions for the simultaneous determination of potassium sorbate, sodium benzoate, carmoisine, allura red, ponceau 4R, and caffeine in commercial soft drinks. The experimental variables chosen were pH (6.0–7.0), flow rate (1.0–1.4 mL/min), and mobile phase ratio (85–95% acetate buffer). Resolution values of all peak pairs were used as a response. Stationary phase was Inertsil OctaDecylSilane- (ODS-) 3V reverse phase column (250 × 4.6 mm, 5 μm) dimensions. The detection was performed at 230 nm. Optimal values were found 6.0 pH, 1.0 mL/min flow rate, and 95% mobile phase ratio for the method which was validated by calculating the linearity (r2>0.9962), accuracy (recoveries ≥ 95.75%), precision (intraday variation ≤ 1.923%, interday variation ≤ 1.950%), limits of detection (LODs), and limits of quantification (LOQs) parameters. LODs and LOQs for analytes were in the range of 0.10–0.19 μg/mL and 0.33–0.63 μg/mL, respectively. The proposed method was applied successfully for the simultaneous determination of the mixtures of five food additives and caffeine in soft drinks.

scholarly journals VALIDATION OF HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF PSEUDOEPHEDRINE HCl, GUAIFENESIN, CHLORPHENIRAMINE MALEATE AND DEXTROMETHORPHAN HBr

2020 ◽  
Author(s):  
Abdrhman Mahmoud Gamil ◽  
Mohammed Awadelkareem Hamad
Keyword(s):  
Peer Review ◽  
Method Development ◽  
Limit Of Detection ◽  
Hplc Method ◽  
Solution Stability ◽  
Chlorpheniramine Maleate ◽  
Validation Parameters ◽  
Review Reports ◽  
Cough Syrup

Objectives: Pseudoephedrine HCl, Guaifenesin, Chlorpheniramine Maleate and Dextromethorphan HBr combination is a common combination cough syrup. Many validated methods are available for the determination of each compound alone and in combination with other drugs. The local pharmaceutical industry used to analyze such combination in individual assessment which is efforts and time consuming. The objective of this study is to validate a method for simultaneous determinations of the four compounds in one single injection. Methods: HPLC method had been develop using detector at 210 nm, column C18 4.6 mm × 250 mm, 3µm and mobile phase of Potassium dihydrogen orthophosphate, acetonitrile, orthophosphoric acid, triethanolamine and water. The column oven temperature is 400C, flow rate 0.8 ml/min and 60 minutes run time. The method had been validated according to the ICH guidelines with respect to method specificity, linearity and range, precision, accuracy and robustness. Limit of detection, quantitation limit and solution stability had been assessed. Results: The average retention times the 4 compounds are 5.5, 12.63, 15.85, 50.44 minutes. The RSD% is less than 1%, the theoretical plates is more than 2000, the tailing factor is not more than 2 and the resolution between the peaks was found to be above 20. The Method showed an appropriate linearity having correlation coefficient r2 0.9996 – 0.9998. The RSD%   of results for two analysts in two different apparatus in two days was less than 2. The test solution is stable for 48 hours. Conclusion: The method is simple and fulfilled all acceptable criteria for all validation parameters. The method is qualified enough to be used for routine analysis of products containing the four components. Peer Review History: Received: 2 September 2020; Revised: 12 October; Accepted: 23 October, Available online: 15 November 2020 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 5.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewer(s) detail: Dr. Vijay Kumar Singh, Institute of Pharmacy, Bundelkhand University, Jhansi, India  [email protected] Dr. Showkat Ahmad Bhawani, UNIMAS Sarawak Malaysia, [email protected]   Comments of reviewer(s): Similar Articles: ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF OMEPRAZOLE AND ASPIRIN USING REVERSE PHASE HPLC METHOD IN BULK AND DOSAGE FORM VALIDATION OF HPLC AND UV VISIBLE METHODS FOR FEW SELECTED BLOOD PRESSURE LOWERING DRUGS AND THEIR FORMULATIONS

scholarly journals Simultaneous Determination of Some Active Ingredients in Cough and Cold Preparations by Gas Chromatography, and Method Validation

2005 ◽  
Vol 88 (4) ◽  
pp. 1093-1098 ◽  
Author(s):  
Thresiana Harsono ◽  
Mochammad Yuwono ◽  
Gunawan Indrayanto
Keyword(s):  
Simultaneous Determination ◽  
Fused Silica ◽  
Chlorpheniramine Maleate ◽  
Column Temperature ◽  
Flame Ionization ◽  
Phenylpropanolamine Hydrochloride ◽  
Fused Silica Capillary ◽  
Silica Capillary Column ◽  
Dextromethorphan Hydrobromide

Abstract A simple and rapid gas chromatographic (GC) method has been developed for the simultaneous determination of combinations of acetaminophen, phenylpropanolamine hydrochloride, guaifenesin, pseudoephedrine hydrochloride, caffeine, chlorpheniramine maleate, and dextromethorphan hydrobromide in cough and cold tablets and syrups. After extraction of the analyte with alkaline ethyl acetate, 2 μL extract was injected (splitting ratio of 50:1) into a gas chromatograph equipped with a CBP1-M25-025 fused silica capillary column (25 m × 0.22 mm; film thickness, 0.25 μm). The column temperature was held at 150°C for 5 min, increased to 175°C at 3°C/min, and increased to 270°C at 10°C/min. The temperatures of the flame ionization detector and injector were maintained at 300°C. The GC method is inexpensive, rapid, accurate, and precise, and thus it can be used for routine analysis of tablet and syrup preparations in quality control laboratories of pharmaceutical companies.

scholarly journals Simultaneous Determination of Phenylephrine Hydrochloride, Guaifenesin, and Chlorpheniramine Maleate in Cough Syrup by Gradient Liquid Chromatography

2008 ◽  
Vol 91 (2) ◽  
pp. 276-284 ◽  
Author(s):  
Sawsan M Amer ◽  
Samah S Abbas ◽  
Mostafa A Shehata ◽  
Nahed M Ali
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Pure Form ◽  
Chlorpheniramine Maleate ◽  
Phenylephrine Hydrochloride ◽  
Cough Syrup ◽  
Liquid Chromatographic

Abstract A simple and reliable high-performance liquid chromatographic method was developed for the simultaneous determination of mixture of phenylephrine hydrochloride (PHENYL), guaifenesin (GUAIF), and chlorpheniramine maleate (CHLO) either in pure form or in the presence of methylparaben and propylparaben in a commercial cough syrup dosage form. Separation was achieved on a C8 column using 0.005 M heptane sulfonic acid sodium salt (pH 3.4 0.1) and acetonitrile as a mobile phase by gradient elution at different flow rates, and detection was done spectrophotometrically at 210 nm. A linear relationship in the range of 30180, 1201800, and 1060 g/mL was obtained for PHENYL, GUAIF, and CHLO, respectively. The results were statistically analyzed and compared with those obtained by applying the British Pharmacopoeia (2002) method and showed that the proposed method is precise, accurate, and can be easily applied for the determination of the drugs under investigation in pure form and in cough syrup formulations.

scholarly journals Stability-indicating RP-HPLC assay for simultaneous determination of chlorpheniramine maleate and prednisolone in veterinary injection

2020 ◽  
Vol 32 (2) ◽  
pp. 122-127
Author(s):  
Amir Ali ◽  
Umar Farooq ◽  
Mahmood Ahmed ◽  
Muhammad Makshoof Athar ◽  
M. Salman ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Degradation Products ◽  
Hplc Method ◽  
Complete Separation ◽  
Calibration Graph ◽  
Coefficient Of Determination ◽  
Prednisolone Acetate ◽  
Chlorpheniramine Maleate

The present work described a simple, rapid, sensitive, accurate, and precise method for simultaneous determination of chlorpheniramine maleate (CHRM) and prednisolone acetate (PRED) in injection samples by high-performance liquid chromatography (HPLC) coupled with UV–Vis detection. Chromatographic separation was accomplished, employing isocratic mode and a mobile phase comprised of acetonitrile and a phosphate buffer (50:50, v/v, 30 °C), adjusted to pH 3.0. The flow rate used was 1.0 mL/min on a Thermo Hypersil ODS C18 column (5 μm, 4.6 × 250 mm), and the injection volume of sample was 20 μL. Analysis of CHRM and PRED was performed at a wavelength of 254 nm. The runtime for analysis was 12.5 min, and the retention times of CHRM and PRED were found to be 2.81 and 5.07 min, respectively. The calibration graph showed linearity over the concentration range 10–70 μg/mL for CHRM and 20–140 μg/mL for PRED with a coefficient of determination (R2) ≥0.9986. Repeatability and reproducibility (expressed as % RSD) were lower than 1.72 and 1.47%, respectively. The proposed HPLC method was demonstrated to be simple and rapid for the determination of CHRM and PRED in injection formulation, providing recoveries between 101.6–102.3%, whereas complete separation of degradation products, from analyte under investigation, provided the specificity of the proposed HPLC method.

A Versatile HPLC Method for the Simultaneous Determination of Bromhexine, Guaifenesin, Ambroxol, Salbutamol/Terbutaline, Pseudoephedrine, Triprolidine, and Chlorpheniramine Maleate in Cough–Cold Syrups

2016 ◽  
Vol 79 (21-22) ◽  
pp. 1507-1514 ◽  
Author(s):  
Paul M. Njaria ◽  
Kennedy O. Abuga ◽  
Franco N. Kamau ◽  
Hezekiah K. Chepkwony
Keyword(s):  
Simultaneous Determination ◽  
Hplc Method ◽  
Chlorpheniramine Maleate

scholarly journals Simple HPLC method for simultaneous determination of acetaminophen, caffeine and chlorpheniramine maleate in tablet formulations

2003 ◽  
Vol 57 (1-2) ◽  
pp. 122-122 ◽  
Author(s):  
M. L. Qi ◽  
P. Wang ◽  
Y. X. Leng ◽  
J. L. Gu ◽  
R. N. Fu
Keyword(s):  
Simultaneous Determination ◽  
Hplc Method ◽  
Chlorpheniramine Maleate ◽  
Tablet Formulations

Simple HPLC method for simultaneous determination of acetaminophen, caffeine and chlorpheniramine maleate in tablet formulations

2002 ◽  
Vol 56 (5-6) ◽  
pp. 295-298 ◽  
Author(s):  
M. L. Qi ◽  
P. Wang ◽  
Y. X. Leng ◽  
J. L. Gu ◽  
R. N. Fu
Keyword(s):  
Simultaneous Determination ◽  
Hplc Method ◽  
Chlorpheniramine Maleate ◽  
Tablet Formulations
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