The Simultaneous Determination of Phenylephrine Hydrochloride, Paracetamol, Chlorpheniramine Maleate and Dextromethorphan Hydrobromide in Pharmaceutical Preparations

2007 ◽  
Vol 66 (S1) ◽  
pp. 93-96 ◽  
Author(s):  
İ. M. Palabıyık ◽  
F. Onur
Keyword(s):  
Simultaneous Determination ◽  
Pharmaceutical Preparations ◽  
Chlorpheniramine Maleate ◽  
Phenylephrine Hydrochloride ◽  
Dextromethorphan Hydrobromide

scholarly journals Simultaneous Determination of Some Active Ingredients in Cough and Cold Preparations by Gas Chromatography, and Method Validation

2005 ◽  
Vol 88 (4) ◽  
pp. 1093-1098 ◽  
Author(s):  
Thresiana Harsono ◽  
Mochammad Yuwono ◽  
Gunawan Indrayanto
Keyword(s):  
Simultaneous Determination ◽  
Fused Silica ◽  
Chlorpheniramine Maleate ◽  
Column Temperature ◽  
Flame Ionization ◽  
Phenylpropanolamine Hydrochloride ◽  
Fused Silica Capillary ◽  
Silica Capillary Column ◽  
Dextromethorphan Hydrobromide

Abstract A simple and rapid gas chromatographic (GC) method has been developed for the simultaneous determination of combinations of acetaminophen, phenylpropanolamine hydrochloride, guaifenesin, pseudoephedrine hydrochloride, caffeine, chlorpheniramine maleate, and dextromethorphan hydrobromide in cough and cold tablets and syrups. After extraction of the analyte with alkaline ethyl acetate, 2 μL extract was injected (splitting ratio of 50:1) into a gas chromatograph equipped with a CBP1-M25-025 fused silica capillary column (25 m × 0.22 mm; film thickness, 0.25 μm). The column temperature was held at 150°C for 5 min, increased to 175°C at 3°C/min, and increased to 270°C at 10°C/min. The temperatures of the flame ionization detector and injector were maintained at 300°C. The GC method is inexpensive, rapid, accurate, and precise, and thus it can be used for routine analysis of tablet and syrup preparations in quality control laboratories of pharmaceutical companies.

scholarly journals Simultaneous Determination of Phenylephrine Hydrochloride, Guaifenesin, and Chlorpheniramine Maleate in Cough Syrup by Gradient Liquid Chromatography

2008 ◽  
Vol 91 (2) ◽  
pp. 276-284 ◽  
Author(s):  
Sawsan M Amer ◽  
Samah S Abbas ◽  
Mostafa A Shehata ◽  
Nahed M Ali
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Pure Form ◽  
Chlorpheniramine Maleate ◽  
Phenylephrine Hydrochloride ◽  
Cough Syrup ◽  
Liquid Chromatographic

Abstract A simple and reliable high-performance liquid chromatographic method was developed for the simultaneous determination of mixture of phenylephrine hydrochloride (PHENYL), guaifenesin (GUAIF), and chlorpheniramine maleate (CHLO) either in pure form or in the presence of methylparaben and propylparaben in a commercial cough syrup dosage form. Separation was achieved on a C8 column using 0.005 M heptane sulfonic acid sodium salt (pH 3.4 0.1) and acetonitrile as a mobile phase by gradient elution at different flow rates, and detection was done spectrophotometrically at 210 nm. A linear relationship in the range of 30180, 1201800, and 1060 g/mL was obtained for PHENYL, GUAIF, and CHLO, respectively. The results were statistically analyzed and compared with those obtained by applying the British Pharmacopoeia (2002) method and showed that the proposed method is precise, accurate, and can be easily applied for the determination of the drugs under investigation in pure form and in cough syrup formulations.

scholarly journals Simultaneous determination of chlorpheniramine maleate, phenylephrine hydrochloride, paracetamol and caffeine in pharmaceutical preparation by RP-HPLC

2013 ◽  
Vol 19 (1) ◽  
pp. 57-65 ◽  
Author(s):  
V.K. Redasani ◽  
A.P. Gorle ◽  
R.A. Badhan ◽  
P.S. Jain ◽  
S.J. Surana
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Pharmaceutical Preparation ◽  
Reversed Phase ◽  
Chlorpheniramine Maleate ◽  
Phenylephrine Hydrochloride ◽  
Rp Hplc ◽  
Relative Standard

Reversed-phase High-Performance Liquid-Chromatography (RP-HPLC) method was successfully developed for the simultaneous determination of quaternary mixture consisting of chlorpheniramine maleate (CPM), phenylephrine hydrochloride (PE), paracetamol (PCM) and caffeine in pharmaceutical preparation. The method was found to be simple, sensitive and rapid. The separation of the drugs was carried out using Inertsil ODS C18 column using 0.05M dibasic phosphate buffer: acetonitrile (93: 07; v/v) as mobile phase. The flow rate of mobile phase was adjusted to 1.5 ml/min and column oven temperature was kept at 30?C. All these drugs were resolved successfully with retention times 2.74 (CPM), 3.48(PE), 9.5(PCM) and 26.32(Caffeine) minutes when detection was carried out at 215 nm. Correlation coefficient was found 0.999, 0.998, 0.999 and 0.999 respectively for CPM, PE, PCM and Caffeine. The relative standard deviation in the tablets was found less than 2% for six replicates. The method was validated for precision and accuracy. Thus, proposed method can be successfully applicable to the pharmaceutical preparation containing the above mentioned drugs without any interference of excipients.

scholarly journals Simultaneous determination of four active pharmaceuticals in tablet dosage form by reversed-phase high performance liquid chromatography

2021 ◽  
Vol 18 (10) ◽  
pp. 2161-2166
Author(s):  
Madhusudan T. Bachute ◽  
Sunil V. Shanbhag ◽  
Shankar L. Turwale
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Dosage Form ◽  
Chlorpheniramine Maleate ◽  
Phenylephrine Hydrochloride ◽  
Limit Of Quantitation ◽  
Tablet Dosage

Purpose: To develop a single, low-cost and rapid analytical method for the simultaneous determination of four active components - chlorpheniramine maleate, paracetamol, phenylephrine hydrochloride and caffeine – in a tablet dosage form. Method: This method was based on reverse-phase high performance liquid chromatography (RPHPLC) and involved the use of a C-18 column (250 × 4.6 mm, 5.0 μm), a mobile phase consisting of buffer solution and methanol at a flow rate of 1.00 mL/min, and gradient determination with UV detection at 220 nm. Results: Retention time was 4.33, 10.36, 13.85, and 17.35 min for phenylephrine hydrochloride, paracetamol, caffeine, and chlorpheniramine maleate, respectively. Specificity data showed no interference from the excipients, and accuracy of the method was close to 100 %. The method was validated as per the guidelines of International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH), and all the results met the acceptance criteria for accuracy, precision, linearity, specificity, limit of quantitation, limit of detection, and robustness. Conclusion: This method can successfully perform quantitative assessment of phenylephrine HCl, chlorpheniramine maleate, paracetamol, and caffeine in tablet combination dosage forms faster and more cost-effectively than conventional methods.

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