scholarly journals Simultaneous Determination of Some Active Ingredients in Cough and Cold Preparations by Gas Chromatography, and Method Validation

2005 ◽  
Vol 88 (4) ◽  
pp. 1093-1098 ◽  
Author(s):  
Thresiana Harsono ◽  
Mochammad Yuwono ◽  
Gunawan Indrayanto
Keyword(s):  
Simultaneous Determination ◽  
Fused Silica ◽  
Chlorpheniramine Maleate ◽  
Column Temperature ◽  
Flame Ionization ◽  
Phenylpropanolamine Hydrochloride ◽  
Fused Silica Capillary ◽  
Silica Capillary Column ◽  
Dextromethorphan Hydrobromide

Abstract A simple and rapid gas chromatographic (GC) method has been developed for the simultaneous determination of combinations of acetaminophen, phenylpropanolamine hydrochloride, guaifenesin, pseudoephedrine hydrochloride, caffeine, chlorpheniramine maleate, and dextromethorphan hydrobromide in cough and cold tablets and syrups. After extraction of the analyte with alkaline ethyl acetate, 2 μL extract was injected (splitting ratio of 50:1) into a gas chromatograph equipped with a CBP1-M25-025 fused silica capillary column (25 m × 0.22 mm; film thickness, 0.25 μm). The column temperature was held at 150°C for 5 min, increased to 175°C at 3°C/min, and increased to 270°C at 10°C/min. The temperatures of the flame ionization detector and injector were maintained at 300°C. The GC method is inexpensive, rapid, accurate, and precise, and thus it can be used for routine analysis of tablet and syrup preparations in quality control laboratories of pharmaceutical companies.

Simultaneous Determination of D- and L-Malic Acids in Apple Juice by Gas Chromatography

1988 ◽  
Vol 71 (3) ◽  
pp. 466-468 ◽  
Author(s):  
Vipin K Agarwal
Keyword(s):  
Gas Chromatography ◽  
Simultaneous Determination ◽  
Apple Juice ◽  
Capillary Column ◽  
Butyl Ester ◽  
Fused Silica ◽  
Fused Silica Capillary ◽  
Silica Capillary Column ◽  
Silica Capillary

Abstract A gas chromatographic (GC) method was developed for the simultaneous determination of D- and L-malic acids in apple juice. D-Malic- (R)-(-)-2-butyl ester and L-malic-(R)-(-)-2-butyl ester are prepared with (R)-(-)-2-butanol. These diastereomers are separated and quantitated by gas chromatography on a Carbowax 20M (30 m x 0.25 mm) fused silica capillary column. This procedure does not involve any extraction or cleanup step.

scholarly journals Development and Validation of Chemometrics-Assisted Spectrophotometry and Liquid Chromatography Methods for the Simultaneous Determination of the Active Ingredients in Two Multicomponent Mixtures Containing Chlorpheniramine Maleate and Phenylpropanolamine Hydrochloride

2007 ◽  
Vol 90 (4) ◽  
pp. 957-970 ◽  
Author(s):  
Ghada M Hadad ◽  
Alaa El-Gindy ◽  
Waleed M M Mahmoud
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Analytical Sensitivity ◽  
Multicomponent Mixtures ◽  
Active Ingredients ◽  
Chlorpheniramine Maleate ◽  
Calibration Methods ◽  
Limit Of Quantitation ◽  
Phenylpropanolamine Hydrochloride

Abstract Multivariate spectrophotometric calibration and liquid chromatography (LC) methods were used for the simultaneous determination of the active ingredients in 2 multicomponent mixtures containing chlorpheniramine maleate and phenylpropanolamine hydrochloride with ibuprofen and caffeine (mixture 1) or with propyphenazone (mixture 2). For the multivariate spectrophotometric calibration methods, principal component regression (PCR) and partial least squares (PLS-1), a calibration set of the mixtures consisting of the components of each mixture was prepared in distilled water. A leave-1-out cross-validation procedure was used to find the optimum numbers of latent variables. Analytical parameters such as sensitivity, selectivity, analytical sensitivity, limit of quantitation, and limit of detection were determined for both PLS-1 and PCR. The LC method depends on the use of a cyanopropyl column with the mobile phase acetonitrile-12 mM ammonium acetate, pH 5.0 (25 + 75, v/v), for mixture 1 or acetonitrile10 mM potassium dihydrogen phosphate, pH 4.7 (45 + 55, v/v), for mixture 2; the UV detector was set at 212 nm. In spite of the presence of a high degree of spectral overlap of these components, they were rapidly and simultaneously determined with high accuracy and precision, with no interference from the matrix excipients. The proposed methods were successfully applied to the analysis of pharmaceutical formulations and laboratory-prepared mixtures containing the 2 multicomponent combinations.

Determination of Ferulic Acid in “AI-FU-NUAN-GONG” Pills by High Performance Capillary Electrophoresis

2013 ◽  
Vol 850-851 ◽  
pp. 57-61
Author(s):  
Hai Xing Liu ◽  
Qing Liu ◽  
Xia Chen ◽  
Yan Fu Huan ◽  
Lin Tong Wang ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
High Performance ◽  
Fused Silica ◽  
Borax Solution ◽  
Fused Silica Capillary ◽  
Capillary Zone ◽  
Good Repeatability ◽  
High Performance Capillary Electrophoresis ◽  
Silica Capillary Column

High performance capillary zone electrophoresis (HPCE) was used in this text to determine the content of ferulic acid in a ready-made Chinese herbal medicine called AI-FUNUAN-GONG (in pills). Electrophoretic separation conditions: Uncoated fused silica capillary column (75um×49/58cm), injection height 7.5cm, 30mmolL-1 borax solution, separation voltage 20kV, injection time 10s, detection wavelength 315 nm, experimental temperature 20°C.Linearity was kept in the concentration ranging from 1~62.5 mg·L-1 of ferulic acid with correlation coefficient of 0.970. The average recovery was 97.541% and RSD value was 6.57%. Sample content of ferulic acid was 0.0715mg/g. This method has the advantages of strong specificity, simpleness, speediness, accurate results and good repeatability in separation and it is useful for determination of ferulic Acid in AI-FU-NUAN-GONG Pills.

Separation and Determination of Diesel Contaminants in Various Fish Products by Capillary Gas Chromatography

1991 ◽  
Vol 74 (6) ◽  
pp. 986-990
Author(s):  
J M Newton ◽  
Barry S Rothman ◽  
F Ann Walker
Keyword(s):  
Diesel Fuel ◽  
Capillary Column ◽  
Fused Silica ◽  
Fish Products ◽  
Seafood Products ◽  
Fused Silica Capillary ◽  
Carry Over ◽  
Gas Chromatographic Method ◽  
Silica Capillary Column

Abstract A semiquantitative capillary column gas chromatographic method Is described for the determination of diesel fuel contamination in various canned seafood products. The diesel contaminants are separated from the fish sample by steam distillation, with little carry-over of interfering intrinsic materials such as fish oils. The diesel fuel is extracted from the condensate with n-hexane, and the extract is analyzed on an SPB-1 fused silica capillary column. The efficiency of recovery of diesel fuel added to canned seafood at levels of 40-400 ppt ranged from 72 to 102%. With the additional step of concentrating the hexane extract, the sensitivity of this procedure may be increased at least 10-fold. This procedure can detect the differences among diesel fuel grades No. 1,2, and 5, and variations within diesel grade No. 2, and thus may be useful in determining the type of petroleum contaminants present in various canned fish products.

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