HIV Type 1 Infection of Human Fetal Bone Marrow Cells Induces Apoptotic Changes in Hematopoietic Precursor Cells and Suppresses Their in Vitro Differentiation and Capacity to Engraft SCID Mice

1999 ◽  
Vol 15 (18) ◽  
pp. 1639-1652 ◽  
Author(s):  
Sergey V. Yurasov ◽  
Massimo Pettoello-Mantovani ◽  
Christina A. Raker ◽  
Harris Goldstein
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Precursor Cells ◽  
Scid Mice ◽  
In Vitro Differentiation ◽  
Hematopoietic Precursor Cells ◽  
Hematopoietic Precursor ◽  
Hiv Type 1

scholarly journals Ultraviolet B light inactivates bone marrow T lymphocytes but spares hematopoietic precursor cells

Blood ◽  
1989 ◽  
Vol 73 (2) ◽  
pp. 369-371
Author(s):  
HJ Deeg ◽  
L Bazar ◽  
M Sigaroudinia ◽  
M Cottler-Fox
Keyword(s):  
Bone Marrow ◽  
T Lymphocytes ◽  
Bone Marrow Cells ◽  
Sharp Decrease ◽  
Precursor Cells ◽  
Ultraviolet B ◽  
Hematopoietic Precursor Cells ◽  
Hematopoietic Precursor ◽  
Macrophage Colony ◽  
Uv Dose

Bone marrow cells from ten normal donors were exposed to ultraviolet (UV)C or UVB light for total exposures of 0.1 to 100 mJ/cm2, and assayed for granulocyte-macrophage colony-forming units (CFU-GM), erythroid burst-forming units (BFU-E), and phytohemagglutinin (PHA)- stimulated proliferative responses. After exposure to UVC CFU-GM, BFU-E and PHA responses showed a UV dose-dependent sharp decrease to levels less than 1% of controls with 0.5, 2.0, and 10 mJ/cm2, respectively. With UVB, PHA responses were most sensitive, declining to less than 1% at 5 mJ/cm2. BFU-E decreased to less than 1% of control with 15 mJ/cm2 UVB. CFU-GM, at UVB doses of 0.1 to 2.0 mJ/cm2, increased to 125% to 130% of control and decreased to less than 1% only at exposures greater than 20 mJ/cm2. Thus, these studies show that UVB, but not UVC light, can be used to inactivate bone marrow T lymphocytes selectively while sparing hematopoietic precursor cells. The data suggest that UVB irradiation can be used for T-lymphocyte purging for allogeneic marrow transplantation.

scholarly journals Ultraviolet B light inactivates bone marrow T lymphocytes but spares hematopoietic precursor cells

Blood ◽  
1989 ◽  
Vol 73 (2) ◽  
pp. 369-371 ◽  
Author(s):  
HJ Deeg ◽  
L Bazar ◽  
M Sigaroudinia ◽  
M Cottler-Fox
Keyword(s):  
Bone Marrow ◽  
T Lymphocytes ◽  
Bone Marrow Cells ◽  
Sharp Decrease ◽  
Precursor Cells ◽  
Ultraviolet B ◽  
Hematopoietic Precursor Cells ◽  
Hematopoietic Precursor ◽  
Macrophage Colony ◽  
Uv Dose

Abstract Bone marrow cells from ten normal donors were exposed to ultraviolet (UV)C or UVB light for total exposures of 0.1 to 100 mJ/cm2, and assayed for granulocyte-macrophage colony-forming units (CFU-GM), erythroid burst-forming units (BFU-E), and phytohemagglutinin (PHA)- stimulated proliferative responses. After exposure to UVC CFU-GM, BFU-E and PHA responses showed a UV dose-dependent sharp decrease to levels less than 1% of controls with 0.5, 2.0, and 10 mJ/cm2, respectively. With UVB, PHA responses were most sensitive, declining to less than 1% at 5 mJ/cm2. BFU-E decreased to less than 1% of control with 15 mJ/cm2 UVB. CFU-GM, at UVB doses of 0.1 to 2.0 mJ/cm2, increased to 125% to 130% of control and decreased to less than 1% only at exposures greater than 20 mJ/cm2. Thus, these studies show that UVB, but not UVC light, can be used to inactivate bone marrow T lymphocytes selectively while sparing hematopoietic precursor cells. The data suggest that UVB irradiation can be used for T-lymphocyte purging for allogeneic marrow transplantation.

scholarly journals A hematopoietic organ-specific 49-kD nuclear antigen: predominance in immature normal and tumor granulocytes and detection in hematopoietic precursor cells

Blood ◽  
1996 ◽  
Vol 87 (6) ◽  
pp. 2283-2291 ◽  
Author(s):  
BC Lee ◽  
Y Shav-Tal ◽  
A Peled ◽  
Y Gothelf ◽  
W Jiang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Western Blotting ◽  
Myeloid Cells ◽  
Precursor Cells ◽  
Immunofluorescent Staining ◽  
Nuclear Antigen ◽  
Hematopoietic Organ ◽  
Hematopoietic Precursor Cells ◽  
Hematopoietic Precursor

A 49-kD protein was specifically detected in hematopoietic organs by Western blotting with a novel mouse monoclonal antibody (B92) raised against stromal cells. The protein was found in the immunizing cells using a sensitive method. However, its detection in the bone marrow by the B92 antibody seemed to stem from the abundance of p49 in immature cells of the myeloid lineage. Study of the bone marrow following in vivo irradiation or 5-fluorouracil (5-FU) treatment, in vitro culture with differentiation-inducing factors and long-term culture, and cell sorting all pointed in the same direction: the protein was found in early myeloid cells and in hematopoietic precursor cells. These results were in accordance with the specific presence of p49 in primary radiation-induced myeloid leukemia and its absence in spontaneous B lymphoma. Immunofluorescent staining using B92 antibody detected a nuclear antigen forming a dotted pattern in early myeloid cells and day 12 colony-forming units-spleen (CFU-S). Nuclear localization of p49 was further demonstrated by subcellular fractionation followed by Western blotting. We thus identified a nuclear protein that within the hematopoietic population is detected in hematopoietic precursor cells, predominates in early myeloid cells, and is reduced following differentiation. These properties imply that p49 might be involved in the regulation of hematopoietic cell growth or differentiation.

In vitro differentiation of human bone marrow stromal cells into neural precursor cells using small molecules

2021 ◽  
Vol 363 ◽  
pp. 109340
Author(s):  
Abeer Sallam ◽  
Thangirala Sudha ◽  
Noureldien H.E. Darwish ◽  
Samar Eghotny ◽  
Abeer E-Dief ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Small Molecules ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Precursor Cells ◽  
Marrow Stromal Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
In Vitro Differentiation

scholarly journals Lymphoid reconstitution of the human fetal thymus in SCID mice with CD34+ precursor cells.

1991 ◽  
Vol 174 (5) ◽  
pp. 1283-1286 ◽  
Author(s):  
B Péault ◽  
I L Weissman ◽  
C Baum ◽  
J M McCune ◽  
A Tsukamoto
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
T Cells ◽  
Bone Marrow Cells ◽  
Fetal Liver ◽  
Precursor Cell ◽  
Scid Mice ◽  
Hematopoietic Stem ◽  
Fetal Thymus

The search for human hematopoietic stem cells has been hampered by the lack of appropriate assay systems. Demonstration of the ability of precursor cell candidates to give rise to T cells is of significant difficulty since dissociated in vitro cultured thymus stroma cells lose their ability to sustain thymocyte maturation. To define further the differentiative capacities of the rare human fetal liver and bone marrow cells that express the CD34 surface antigen and exhibit in vitro myeloid and pre-B cell activities, we have microinjected them into HLA-mismatched fetal thymus fragments, partially depleted of hematopoietic cells by low temperature culture. In vitro colonized thymuses have then been allowed to develop upon engraftment into immunodeficient SCID mice. Using this modification of the SCID-hu system, we show that low numbers of fetal CD34+ progenitor cells can repopulate the lymphoid compartment in the human thymus.

scholarly journals Monoclonal antibody BA-1 does not bind to hematopoietic precursor cells

Blood ◽  
1982 ◽  
Vol 59 (5) ◽  
pp. 1029-1035 ◽  
Author(s):  
J Jansen ◽  
RC Ash ◽  
ED Zanjani ◽  
TW LeBien ◽  
JH Kersey
Keyword(s):  
Monoclonal Antibody ◽  
Bone Marrow ◽  
Progenitor Cells ◽  
Bone Marrow Cells ◽  
Lymphoblastic Leukemia ◽  
Autologous Bone Marrow Transplantation ◽  
Autologous Bone Marrow ◽  
Hematopoietic Precursor ◽  
Proliferation In Vitro

Abstract Monoclonal antibody BA-1 binds to B lymphocytes, to cells from most cases of non-T acute lymphoblastic leukemia (ALL), and weakly to neutrophils. To determine whether BA-1 also reacts with hematopoietic progenitor cells (HPC), we studied the effect of removal of BA-1+ cells from human bone marrow on the proliferation in vitro of the trilineage precursor cell CFU-GEMM, and on the committed progenitor cells of granulopoiesis (CFU-C) and erythropoiesis (BFU-E/CFU-E). Complement- mediated cytotoxicity using BA-1 at concentrations far beyond those required to lyse BA-1+ bone marrow cells and ALL cells did not result in inhibition of colony formation in any of the assays. A rosette separation method, using ox red blood cells coated with BA-1, resulted in enrichment of HPC in the BA-1-depleted interface, whereas very few HPC were found in the BA-1-enriched pellet. Both methods indicate that BA-1 does not bind to HPC, although binding of the antibody to the lymphohematopoietic stem cell cannot be excluded yet. The high cytotoxic capacity of the IgM antibody BA-1, and the lack of reactivity with HPC, make the antibody particularly suitable for use in autologous bone marrow transplantation for patients with ALL.

scholarly journals Physalis angulata induces in vitro differentiation of murine bone marrow cells into macrophages

2014 ◽  
Vol 15 (1) ◽  
Author(s):  
Bruno José Martins da Silva ◽  
Ana Paula D Rodrigues ◽  
Luis Henrique S Farias ◽  
Amanda Anastácia P Hage ◽  
Jose Luiz M Do Nascimento ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
In Vitro Differentiation ◽  
Murine Bone Marrow ◽  
Physalis Angulata ◽  
Marrow Cells

Noninvasive MR imaging of magnetically labeled stem cells to directly identify neovasculature in a glioma model

Blood ◽  
2005 ◽  
Vol 105 (1) ◽  
pp. 420-425 ◽  
Author(s):  
Stasia A. Anderson ◽  
John Glod ◽  
Ali S. Arbab ◽  
Martha Noel ◽  
Parwana Ashari ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Bone Marrow ◽  
Magnetic Resonance ◽  
Gene Delivery ◽  
Bone Marrow Cells ◽  
Precursor Cells ◽  
Resonance Imaging ◽  
Gadolinium Contrast

Abstract Bone marrow-derived endothelial precursor cells incorporate into neovasculature and have been successfully used as vehicles for gene delivery to brain tumors. To determine whether systemically administered Sca1+ bone marrow cells labeled with superparamagnetic iron oxide nanoparticles can be detected by in vivo magnetic resonance imaging in a mouse brain tumor model, mouse Sca1+ cells were labeled in vitro with ferumoxides-poly-l-lysine complexes. Labeled or control cells were administered intravenously to glioma-bearing severe combined immunodeficient (SCID) mice. Magnetic resonance imaging (MRI) was performed during tumor growth. Mice that received labeled cells demonstrated hypointense regions within the tumor that evolved over time and developed a continuous dark hypointense ring at a consistent time point. This effect was not cleared by administration of a gadolinium contrast agent. Histology showed iron-labeled cells around the tumor rim in labeled mice, which expressed CD31 and von Willebrand factor, indicating the transplanted cells detected in the tumor have differentiated into endothelial-like cells. These results demonstrate that MRI can detect the incorporation of magnetically labeled bone marrow-derived precursor cells into tumor vasculature as part of ongoing angiogenesis and neovascularization. This technique can be used to directly identify neovasculature in vivo and to facilitate gene therapy by noninvasively monitoring these cells as gene delivery vectors. (Blood. 2005;105:420-425)

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