Oxidative stress and mitochondrial dysfunction associated with peripheral neuropathy in type 1 diabetes

2021 ◽  
Author(s):  
Eftekhar Eftekharpour ◽  
Paul Fernyhough
Keyword(s):  
Oxidative Stress ◽  
Type 1 Diabetes ◽  
Peripheral Neuropathy ◽  
Mitochondrial Dysfunction

scholarly journals Elucidation of Molecular Mechanisms of Streptozotocin-Induced Oxidative Stress, Apoptosis, and Mitochondrial Dysfunction in Rin-5F Pancreatic β-Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-15 ◽  
Author(s):  
Arwa M. T. Al Nahdi ◽  
Annie John ◽  
Haider Raza
Keyword(s):  
Oxidative Stress ◽  
Type 1 Diabetes ◽  
Mitochondrial Dysfunction ◽  
Molecular Mechanisms ◽  
Pancreatic Beta Cell ◽  
Dependent Manner ◽  
Respiratory Enzymes ◽  
Altered Expression ◽  
Pancreatic Cancer Cells

Streptozotocin is a pancreatic beta-cell-specific cytotoxin and is widely used to induce experimental type 1 diabetes in rodent models. The precise molecular mechanism of STZ cytotoxicity is however not clear. Studies have suggested that STZ is preferably absorbed by insulin-secreting β-cells and induces cytotoxicity by producing reactive oxygen species/reactive nitrogen species (ROS/RNS). In the present study, we have investigated the mechanism of cytotoxicity of STZ in insulin-secreting pancreatic cancer cells (Rin-5F) at different doses and time intervals. Cell viability, apoptosis, oxidative stress, and mitochondrial bioenergetics were studied. Our results showed that STZ induces alterations in glutathione homeostasis and inhibited the activities of the respiratory enzymes, resulting in inhibition of ATP synthesis. Apoptosis was observed in a dose- and time-dependent manner. Western blot analysis has also confirmed altered expression of oxidative stress markers (e.g., NOS and Nrf2), cell signaling kinases, apoptotic protein-like caspase-3, PARP, and mitochondrial specific proteins. These results suggest that STZ-induced cytotoxicity in pancreatic cells is mediated by an increase in oxidative stress, alterations in cellular metabolism, and mitochondrial dysfunction. This study may be significant in better understanding the mechanism of STZ-induced β-cell toxicity/resistance and the etiology of type 1 diabetes induction.

Impaired awareness of hypoglycemia in adults with type 1 diabetes is not associated with peripheral neuropathy

2017 ◽  
Vol 12 (S 01) ◽  
pp. S1-S84
Author(s):  
D Tsitlakidis ◽  
M Djordjevic ◽  
N Müller ◽  
UA Müller ◽  
C Kloos
Keyword(s):  
Type 1 Diabetes ◽  
Peripheral Neuropathy ◽  
Impaired Awareness

Type 1 diabetes and oxidative stress markers

2021 ◽  
Vol 242 ◽  
pp. 174
Author(s):  
Widad Sobhi ◽  
Rania Derguine ◽  
Saliha Boucheffa ◽  
Abdelhalim Khenchouche ◽  
Nada Boutrid ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 1 Diabetes ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
And Oxidative Stress

Glutathione peroxidase-1 gene (GPX1) variants, oxidative stress and risk of kidney complications in people with type 1 diabetes

Metabolism ◽  
2016 ◽  
Vol 65 (2) ◽  
pp. 12-19 ◽  
Author(s):  
Kamel Mohammedi ◽  
Thiago A. Patente ◽  
Naima Bellili-Muñoz ◽  
Fathi Driss ◽  
Hervé Le Nagard ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 1 Diabetes ◽  
Glutathione Peroxidase ◽  
Glutathione Peroxidase 1

Oxidative Stress in Type 1 Diabetes

2005 ◽  
pp. 319-344
Author(s):  
Robert Hoeldtke
Keyword(s):  
Oxidative Stress ◽  
Type 1 Diabetes

scholarly journals Concentration of 8-isoprostanes in the exhaled breath condensate as a marker of oxidative stress in patients with type 1 diabetes

2018 ◽  
Vol 86 (1) ◽  
pp. 3-6 ◽  
Author(s):  
Anna Pękala-Wojciechowska ◽  
Michał Poznański ◽  
Kamil Szyszow ◽  
Adam Antczak
Keyword(s):  
Oxidative Stress ◽  
Type 1 Diabetes ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Breath Condensate

scholarly journals Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes

2020 ◽  
Vol 9 (7) ◽  
pp. 2155
Author(s):  
Francesca Iannantuoni ◽  
Aranzazu M. de Marañon ◽  
Zaida Abad-Jiménez ◽  
Francisco Canet ◽  
Pedro Díaz-Pozo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 1 Diabetes ◽  
Adhesion Molecules ◽  
Mitochondrial Function ◽  
Mitochondrial Membrane ◽  
Diabetic Patients ◽  
Ros Production ◽  
Mitochondrial Ros ◽  
Type 1 Diabetic Patients

Type 1 diabetes has been associated with oxidative stress. This study evaluates the rates of oxidative stress, mitochondrial function, leukocyte–endothelium interactions and adhesion molecules in type 1 diabetic patients. The study population consisted of 52 diabetic patients and 46 body-composition and age-matched controls. We assessed anthropometric and metabolic parameters, oxidative stress and mitochondrial function by evaluating reactive oxygen species (ROS) production, mitochondrial ROS production, mitochondrial membrane potential and superoxide dismutase (SOD) and catalase (CAT) expression in polymorphonuclear leukocytes from type 1 diabetic patients. In addition, we evaluated interactions between leukocytes and human umbilical vein endothelial cells (HUVEC), and serum expression of adhesion molecules (P-selectin, VCAM-1 and ICAM-1), proinflammatory cytokines (IL-6 and TNFα) and myeloperoxidase (MPO). HbA1C and glucose levels were higher in diabetic patients than in control subjects, as expected. Mitochondrial function was altered and leukocyte–endothelium interactions were enhanced in diabetic patients, which was evident in the increase in total and mitochondrial ROS production, higher mitochondrial membrane potential, enhanced leukocyte rolling and adhesion, and decreased rolling velocity. Furthermore, we observed an increase in levels of adhesion molecules P-selectin, VCAM-1, and ICAM-1 in these subjects. In addition, type 1 diabetic patients exhibited an increase in proinflammatory mediators TNFα and MPO, and a decreased expression of SOD. The enhancement of leukocyte–endothelium interactions and proinflammatory markers correlated with glucose and HbA1Clevels. Mitochondrial alteration, oxidative stress, and enhanced leukocyte–endothelium interactions are features of type 1 diabetes and may be related to cardiovascular implications.

Sign in / Sign up

Export Citation Format

Share Document