Prevalence of Zoonotic or Potentially Zoonotic Bacteria, Antimicrobial Resistance, and Somatic Cell Counts in Organic Dairy Production: Current Knowledge and Research Gaps

Mastitis is a complex disease causing inflammation of the udder, which has been estimated to cost the dairy farmer between £40-£117/cow per year (Stott et al., 2002). Economic loss occurs as a result of discarded milk, reduced milk yield and milk quality, increased vet costs and an increase in replacement costs. The objective of this study was to examine the effect of breed on the incidence of mastitis and somatic cell counts and milk production capabilities of Holstein Friesian, Brown Swiss and Brown Swiss crossbred cows.

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Extensive Countrywide Field Investigation of Somatic Cell Counts and Total Bacterial Counts in Bulk-Tank Raw Milk in Sheep Flocks in Greece

Foods ◽

10.3390/foods10020268 ◽

2021 ◽

Vol 10 (2) ◽

pp. 268

Author(s):

Daphne T. Lianou ◽

Charalambia K. Michael ◽

Natalia G. C. Vasileiou ◽

Efthymia Petinaki ◽

Peter J. Cripps ◽

...

Keyword(s):

Somatic Cell ◽

Geometric Mean ◽

Cell Counting ◽

Lactation Period ◽

Mechanical Ventilators ◽

Bulk Tank Milk ◽

Bacterial Counts ◽

Somatic Cell Counts ◽

Cell Counts ◽

Bulk Tank

Objectives were to investigate somatic cell counts (SCC) and total bacterial counts (TBC) in the raw bulk-tank milk of sheep flocks in Greece, to study factors potentially influencing increased SCC and TBC in the bulk-tank milk of sheep and to evaluate possible associations of SCC and TBC with milk content. Throughout Greece, 325 dairy sheep flocks were visited for collection of milk sampling for somatic cell counting, microbiological examination and composition measurement. Geometric mean SCC were 0.488 × 106 cells mL−1; geometric mean TBC were 398 × 103 cfu mL−1; 228 staphylococcal isolates were recovered form 206 flocks (63.4%). Multivariable analyses revealed annual incidence risk of clinical mastitis, age of the farmer and month into lactation period (among 53 variables) to be significant for SCC > 1.0 × 106 cells mL−1 and month into lactation period at sampling and availability of mechanical ventilators (among 58 variables) to be significant for TBC > 1500 × 103 cfu mL−1. Negative correlation of SCC with fat, total protein and lactose and positive correlation of SCC with added water were found. With SCC > 1.0 × 106 cells mL−1, significant reduction of protein content (2%) was observed, whilst in flocks with SCC > 1.5 × 106 cells mL−1, significantly lower annual milk production per ewe (42.9%) was recorded.

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Carryover of aflatoxin from feed to milk in dairy cows with low or high somatic cell counts

animal ◽

10.1017/s1751731107000663 ◽

2007 ◽

Vol 1 (9) ◽

pp. 1344-1350 ◽

Cited By ~ 70

Author(s):

F. Masoero ◽

A. Gallo ◽

M. Moschini ◽

G. Piva ◽

D. Diaz

Keyword(s):

Dairy Cows ◽

Somatic Cell ◽

Somatic Cell Counts ◽

Cell Counts

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Factors Associated with High Milk Test Day Somatic Cell Counts in Large Dairy Herds in Brandenburg. II. Milking Practices

Journal of Veterinary Medicine Series A ◽

10.1111/j.1439-0442.2006.00814.x ◽

2006 ◽

Vol 53 (4) ◽

pp. 209-214 ◽

Cited By ~ 10

Author(s):

G. Koster ◽

B.-A. Tenhagen ◽

N. Scheibe ◽

W. Heuwieser

Keyword(s):

Somatic Cell ◽

Dairy Herds ◽

Somatic Cell Counts ◽

Factors Associated ◽

Cell Counts

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Bayesian estimation of diagnostic accuracy of somatic cell counts history and on-farm milk culture using Petrifilm® to identify quarters or cows that should be treated with antimicrobials in selective treatment protocols at dry off

Preventive Veterinary Medicine ◽

10.1016/j.prevetmed.2021.105452 ◽

2021 ◽

pp. 105452

Author(s):

Fidèle Kabera ◽

Jean-Philippe Roy ◽

Greg Keefe ◽

Simon Dufour

Keyword(s):

Diagnostic Accuracy ◽

Somatic Cell ◽

Bayesian Estimation ◽

Selective Treatment ◽

Treatment Protocols ◽

Somatic Cell Counts ◽

Cell Counts ◽

On Farm

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Rapid Detection of Mycoplasma bovis, Staphylococcus aureus and Streptococcus agalactiae in Cattle Bulk Tank Milk in Cyprus and Relations with Somatic Cell Counts

Pathogens ◽

10.3390/pathogens10070841 ◽

2021 ◽

Vol 10 (7) ◽

pp. 841

Author(s):

Maria Liapi ◽

George Botsaris ◽

Costas Arsenoglou ◽

Nikolas Markantonis ◽

Christodoulos Michael ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Somatic Cell ◽

Real Time ◽

Streptococcus Agalactiae ◽

Mycoplasma Bovis ◽

Bulk Tank Milk ◽

Somatic Cell Counts ◽

Milk Samples ◽

Cell Counts ◽

Bulk Tank

One hundred and seventy-seven (177) bulk tank milk samples were analyzed with a commercially available real-time polymerase chain reaction kit and 11 (6.21%), 41 (23.16%), and 58 (32.77%) tested positive for Mycoplasma bovis, Staphylococcus aureus, and Streptococcus agalactiae, respectively. Statistical analysis revealed a significant relationship between the presence of S. aureus and S. agalactiae. Enumeration of somatic cells was performed in the same samples by flow cytometry. The somatic cell counts were found higher in S. aureus and S. agalactiae positive samples. No association was found between M. bovis presence and somatic cells counts. Low internal assay control Ct values were found to be related with high somatic cell counts. Noticeably, this is the first report for the presence of M. bovis in Cyprus. Therefore, its presence was confirmed by bulk tank milk culture, conventional PCR, and next generation sequencing. Furthermore, M. bovis was typed with multilocus sequencing typing and was allocated to sequence type 29 (ST 29). Real-time PCR in bulk tank milk samples is a useful tool to detect mammary infections, especially for neglected pathogens such as M. bovis.

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