Both Gfrα1a and Gfrα1b Are Involved in the Self-Renewal and Maintenance of Spermatogonial Stem Cells in Medaka

ABSTRACTThe self-renewal of mammalian spermatogonial stem cells (SSCs) supports spermatogenesis to produce spermatozoa, and this is precisely controlled in a stem niche microenvironment in the seminiferous tubules. Although studies have revealed the role of the surrounding factors in SSCs, little is known about whether the division of SSCs is controlled by extracellular vesicles. Here, extracellular vesicles were found in the basal compartment of seminiferous tubules in mouse, rat, rabbit and human testes. In the mice, the testicular extracellular vesicles are secreted by spermatogonia and are taken up by SSCs. Further, the extracellular vesicles from thy1-positive spermatogonia were purified by anti-Thy1-coupled magnetic beads, and which suppress their proliferation of SSCs but not lead to the apoptosis in vitro.

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The involvement of bioactive factors in the self-renewal and stemness maintenance of spermatogonial stem cells

Molecular and Cellular Biochemistry ◽

10.1007/s11010-020-04028-7 ◽

2021 ◽

Author(s):

Guoqing Yang ◽

Yuqing He ◽

Hao Yang

Keyword(s):

Stem Cells ◽

Spermatogonial Stem Cells ◽

The Self ◽

Self Renewal

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Thy1-positive spermatogonia suppress the proliferation of spermatogonial stem cells by Extracellular vesicles in vitro

Endocrinology ◽

10.1210/endocr/bqab052 ◽

2021 ◽

Author(s):

Lin Yu ◽

Qian Fang ◽

Yue He ◽

Xiaowen Gong ◽

Yinjuan Wang ◽

...

Keyword(s):

Stem Cells ◽

Extracellular Vesicles ◽

Magnetic Beads ◽

Spermatogonial Stem Cells ◽

The Self ◽

Seminiferous Tubules ◽

Self Renewal

Abstract The self-renewal of mammalian spermatogonial stem cells (SSCs) supports spermatogenesis to produce spermatozoa, and this is precisely controlled in a stem niche microenvironment in the seminiferous tubules. Although studies have revealed the role of the surrounding factors in SSCs, little is known about whether the division of SSCs is controlled by extracellular vesicles. Here, extracellular vesicles were found in the basal compartment of seminiferous tubules in mouse, rat, rabbit and human testes. In the mice, the testicular extracellular vesicles are secreted by spermatogonia and are taken up by SSCs. Further, the extracellular vesicles from thy1-positive spermatogonia were purified by anti-Thy1-coupled magnetic beads, and which suppress their proliferation of SSCs but not lead to the apoptosis in vitro.

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Hsa-miR-1908-3p Mediates the Self-Renewal and Apoptosis of Human Spermatogonial Stem Cells via Targeting KLF2

Molecular Therapy — Nucleic Acids ◽

10.1016/j.omtn.2020.04.016 ◽

2020 ◽

Vol 20 ◽

pp. 788-800

Author(s):

Wei Chen ◽

Yinghong Cui ◽

Bang Liu ◽

Chunyun Li ◽

Li Du ◽

...

Keyword(s):

Stem Cells ◽

Spermatogonial Stem Cells ◽

The Self ◽

Self Renewal

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Chemokine (C-X-C) Ligand 12 Facilitates Trafficking of Donor Spermatogonial Stem Cells

Stem Cells International ◽

10.1155/2016/5796305 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 12

Author(s):

Zhiyv Niu ◽

Shaun M. Goodyear ◽

Mary R. Avarbock ◽

Ralph L. Brinster

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Primordial Germ Cells ◽

Critical Role ◽

Spermatogonial Stem Cells ◽

The Self ◽

Self Renewal ◽

Critical Window ◽

Cxcr4 Signaling

The chemokine (C-X-C) receptor type 4 (CXCR4) is an early marker of primordial germ cells (PGCs) essential for their migration and colonization of the gonads. In spermatogonial stem cells (SSCs), the expression of CXCR4 is promoted by the self-renewal factor, glial cell line-derived neurotrophic factor (GDNF). Here, we demonstrate an important role of CXCR4 during donor mouse SSCs reoccupation of the endogenous niche in recipient testis. Silencing of CXCR4 expression in mouse SSCs dramatically reduced the number of donor stem cell-derived colonies, whereas colony morphology and spermatogenesis were comparable to controls. Inhibition of CXCR4 signaling using a small molecule inhibitor (AMD3100) during the critical window of homing also significantly lowered the efficiency of donor-derived SSCs to establish spermatogenic colonies in recipient mice; however, the self-renewal of SSCs was not affected by exposure to AMD3100. Rather,in vitromigration assays demonstrate the influence of CXCR4-CXCL12 signaling in promoting germ cell migration. Together, these studies suggest that CXCR4-CXCL12 signaling functions to promote homing of SSCs towards the stem cell niche and plays a critical role in reestablishing spermatogenesis.

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