Chondrogenic Differentiation Potential of Human Mesenchymal Stem Cells Photoencapsulated within Poly(Ethylene Glycol)–Arginine-Glycine-Aspartic Acid-Serine Thiol-Methacrylate Mixed-Mode Networks

2007 ◽  
Vol 13 (5) ◽  
pp. 1025-1034 ◽  
Author(s):  
Chelsea N. Salinas ◽  
Brook B. Cole ◽  
Andrea M. Kasko ◽  
Kristi S. Anseth
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
Mixed Mode ◽  
Chondrogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Poly Ethylene Glycol ◽  
Differentiation Potential ◽  
Poly Ethylene ◽  
Arginine Glycine Aspartic Acid

scholarly journals Osteogenic differentiation of human mesenchymal stem cells on poly(ethylene glycol)-variant biomaterials

2009 ◽  
Vol 91A (4) ◽  
pp. 975-984 ◽  
Author(s):  
Tonye Briggs ◽  
Matthew D. Treiser ◽  
Paul F. Holmes ◽  
Joachim Kohn ◽  
Prabhas V. Moghe ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Effect of Surface Electrostatic Property on Chondrogenic Differentiation of Human Mesenchymal Stem Cells

2007 ◽  
Vol 330-332 ◽  
pp. 1189-1192
Author(s):  
Li Guo ◽  
Yu Jiang Fan ◽  
Naoki Kawazoe ◽  
Guo Ping Chen ◽  
M. Tanaka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Acrylic Acid ◽  
Chondrogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Poly Ethylene Glycol ◽  
Fast Green ◽  
Reactive Polymers ◽  
Poly Ethylene ◽  
Safranin O

In this study, three kinds of photo-reactive polymers, namely positively charged azidophenyl-derived polyallylamine (AzPhPAAm), negatively charged azidophenyl-derived poly(acrylic acid) (AzPhPAAc) and neutral azidophenyl-derived poly(ethylene glycol) (AzPhPEG), were synthesized by introduction of photo-reactive group (-N3) into polyallylamine, poly(acrylic acid) and poly(ethylene glycol), respectively. The structure of the azidophenyl-derived polymers was confirmed by 1H-NMR measurement. All of the photo-reactive polymers can be pattern-grafted on the surface of cells culture plate, which was confirmed by the optical microscopy observation. The contact angle decreased after surface modification by the photo-reactive polymers. Human mesenchymal stem cells (MSC) cultured on the modified surfaces showed different morphology. The cells adhered and spread more on the PAAm- and PAAc-grafted surfaces than on the PEG-grafted surface. The pellets formed on PAAm- and PEG-grafted surfaces in condrogenic differentiation medium were positively stained by safranin O/ fast green. But the pellet formed on PAAc-grafted surface did not show obviously positive staining for safranin O/ fast green. These results indicate that the PAAm- and PEG-grafted surfaces promoted chondrogenic differentiation of MSC.

scholarly journals Osteogenic potential of poly(ethylene glycol)-amorphous calcium phosphate composites on human mesenchymal stem cells

2020 ◽  
Vol 11 ◽  
pp. 204173142092684 ◽  
Author(s):  
Aman S Chahal ◽  
Manuel Schweikle ◽  
Aina-Mari Lian ◽  
Janne E Reseland ◽  
Håvard J Haugen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Ethylene Glycol ◽  
Osteogenic Differentiation ◽  
Amorphous Calcium Phosphate ◽  
Human Mesenchymal Stem Cells ◽  
Osteogenic Potential ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Synthetic hydrogel-amorphous calcium phosphate composites are promising candidates to substitute biologically sourced scaffolds for bone repair. While the hydrogel matrix serves as a template for stem cell colonisation, amorphous calcium phosphate s provide mechanical integrity with the potential to stimulate osteogenic differentiation. Here, we utilise composites of poly(ethylene glycol)-based hydrogels and differently stabilised amorphous calcium phosphate to investigate potential effects on attachment and osteogenic differentiation of human mesenchymal stem cells. We found that functionalisation with integrin binding motifs in the form of RGD tripeptide was necessary to allow adhesion of large numbers of cells in spread morphology. Slow dissolution of amorphous calcium phosphate mineral in the scaffolds over at least 21 days was observed, resulting in the release of calcium and zinc ions into the cell culture medium. While we qualitatively observed an increasingly mineralised extracellular matrix along with calcium deposition in the presence of amorphous calcium phosphate-loaded scaffolds, we did not observe significant changes in the expression of selected osteogenic markers.

Structural engineering to control density, conformation, and bioactivity of the poly(ethylene glycol)-grafted poly(urethane urea) scaffolds

2018 ◽  
Vol 34 (2) ◽  
pp. 209-223
Author(s):  
Shideh Shaneh ◽  
Fatemeh Shokrolahi ◽  
Parvin Shokrollahi ◽  
Hamid Yeganeh ◽  
Hossein Omidian
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
Structural Engineering ◽  
Cell Attachment ◽  
Poly Ethylene Glycol ◽  
Salt Leaching ◽  
The Matrix ◽  
Diphenyl Tetrazolium Bromide ◽  
Poly Ethylene

Poly(urethane urea) scaffolds were fabricated through combined salt leaching and solvent casting methods. The scaffolds were then functionalized via aminolysis with poly(ethylene glycol) (PEG- g-PUU). To compare its bioactivity, gelatin was also grafted onto the aminolyzed poly(urethane urea) surface (Gel- g-PUU). Chemical changes at the surface were then monitored using quantitative/qualitative methods. Grafting with both gelatin and poly(ethylene glycol) remarkably enhanced the wettability of poly(urethane urea). Proliferation of human adipose–derived mesenchymal stem cells on poly(urethane urea) and the modified poly(urethane urea)s was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay. The cell experiment results showed that both the modified poly(urethane urea)s enhanced the attachment and proliferation of human adipose–derived mesenchymal stem cells compared to pure poly(urethane urea). Based on previous reports, while a supportive role is observed at adequate poly(ethylene glycol) graft densities, cell adhesion and proliferation are inhibited at very high grafting densities. To correlate the cell data to poly(ethylene glycol) conformations, the surface tension was measured. Data on human adipose–derived mesenchymal stem cells’ attachment/proliferation and contact angle/surface free energy together showed that the grafting density of poly(ethylene glycol) was regulated by optimizing aminolysis conditions, careful selection of poly(ethylene glycol)’s molecular weight, and bulk properties of the matrix poly(urethane urea). As a result, surface overcrowding and brush conformation of the poly(ethylene glycol) chains were avoided, and human adipose–derived mesenchymal stem cell attachment and proliferation occurred on the PEG- g-PUU scaffold at a comparable level to the Gel- g-PUU.

scholarly journals Poly(3-hydroxybutyrate)/poly(ethylene glycol) scaffolds with different microstructure: the effect on growth of mesenchymal stem cells

3 Biotech ◽  
2018 ◽  
Vol 8 (8) ◽  
Author(s):  
A. P. Bonartsev ◽  
I. I. Zharkova ◽  
V. V. Voinova ◽  
E. S. Kuznetsova ◽  
V. A. Zhuikov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Effect On Growth
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