scholarly journals A Three-Dimensional In Vitro Coculture Model to Quantify Breast Epithelial Cell Adhesion to Endothelial Cells

2019 ◽  
Vol 25 (10) ◽  
pp. 609-618 ◽  
Author(s):  
Swathi Swaminathan ◽  
Aaron N. Cranston ◽  
Alisa Morss Clyne
Keyword(s):  
Endothelial Cells ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Three Dimensional ◽  
Breast Epithelial Cell ◽  
Coculture Model ◽  
Breast Epithelial

scholarly journals β-alanine suppresses malignant breast epithelial cell aggressiveness through alterations in metabolism and cellular acidity in vitro

2014 ◽  
Vol 13 (1) ◽  
pp. 14 ◽  
Author(s):  
Roger A Vaughan ◽  
Nicholas P Gannon ◽  
Randi Garcia-Smith ◽  
Yamhilette Licon-Munoz ◽  
Miguel A Barberena ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Breast Epithelial Cell ◽  
Malignant Breast ◽  
Breast Epithelial

scholarly journals The in vitro effects of 2-methoxyestradiol-bis-sulphamate on cell numbers, membrane integrity and cell morphology, and the possible induction of apoptosis and autophagy in a non-tumorigenic breast epithelial cell line

2010 ◽  
Vol 15 (4) ◽  
Author(s):  
Michelle Visagie ◽  
Annie Joubert
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Membrane Integrity ◽  
Breast Epithelial Cell ◽  
Epithelial Cell Line ◽  
Cell Numbers ◽  
Breast Epithelial Cell Line ◽  
Induction Of Apoptosis ◽  
Breast Epithelial

Abstract2-methoxyestradiol (2ME2) exerts estrogen receptor-independent anti-proliferative, anti-angiogenic and anti-tumor activity in vitro and in vivo. Due to its low bioavailability and rapid metabolic degradation, several analogues have been developed in recent years. 2-methoxyestradiol-bis-sulphamate (2-MeOE2bisMATE) is a bis-sulphamoylated derivative of 2ME2 with anti-proliferative activity. The aim of this study was to investigate cell signaling events induced by 2-MeOE2bisMATE in a non-tumorigenic cell line (MCF-12A) by analysing its influence on cell number, morphology and membrane integrity, and the possible induction of apoptosis and autophagy. Dose- and time-dependent studies revealed that 48 h exposure to 2-MeOE2bisMATE (0.4 μM) resulted in a decrease in cell numbers to 79%. A slight increase in the level of lactate dehydrogenase production was observed in the 2-MeOE2bisMATE-treated cells. Morphological studies revealed an increase in the number of cells in metaphase. Hallmarks of apoptosis were also found, namely nuclear fragmentation and apoptotic bodies. In addition, increased lysosomal staining was observed via fluorescent microscopy, suggesting the induction of another type of cell death, namely autophagy. Since 2-MeOE2bisMATE is regarded as a potential anti-cancer agent, it is also imperative to investigate the susceptibility of non-tumorigenic cells to its influence. The data generated from this study contributes to the understanding of the action that 2-MeOE2bisMATE exerts on the non-tumorigenic MCF-12A breast epithelial cell line.

scholarly journals Enhancement of Endothelial Cell Migration and in Vitro Tube Formation by Tap20, a Novel β5 Integrin–Modulating, Pkcθ-Dependent Protein

1999 ◽  
Vol 147 (5) ◽  
pp. 1073-1084 ◽  
Author(s):  
Shaoqing Tang ◽  
Yunling Gao ◽  
J. Anthony Ware
Keyword(s):  
Endothelial Cells ◽  
Cell Adhesion ◽  
Adhesion Formation ◽  
Three Dimensional ◽  
Tube Formation ◽  
Endothelial Cell Migration ◽  
Kinase C ◽  
Focal Adhesion Formation ◽  
Dependent Protein

Migration, proliferation, and tube formation of endothelial cells are regulated by a protein kinase C isoenzyme PKCθ. A full-length cDNA encoding a novel 20-kD protein, whose expression was PKCθ-dependent, was identified in endothelial cells, cloned, characterized, and designated as theta-associated protein (TAP) 20. Overexpression of TAP20 decreased cell adhesion and enhanced migration on vitronectin and tube formation in three-dimensional culture. An antiintegrin αvβ5 antibody prevented these TAP20 effects. Overexpression of TAP20 also decreased focal adhesion formation in αvβ3-deficient cells. The interaction between TAP20 and β5 integrin cytoplasmic domain was demonstrated by protein coprecipitation and immunoblotting. Thus, the discovery of TAP20, which interacts with integrin β5 and modulates cell adhesion, migration, and tube formation, further defines a possible pathway to angiogenesis dependent on PKCθ.

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