High-Throughput Culture Method of Induced Pluripotent Stem Cell Derived Alveolar Epithelial Cells

2021 ◽  
Author(s):  
David Becerra ◽  
Tong Wu ◽  
Sydney Jeffs ◽  
Harald C. Ott
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
High Throughput ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Culture Method ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Induced Pluripotent

Human induced pluripotent stem cell–derived lung progenitor and alveolar epithelial cells attenuate hyperoxia-induced lung injury

Cytotherapy ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 108-125 ◽  
Author(s):  
Mehdi Shafa ◽  
Lavinia Iuliana Ionescu ◽  
Arul Vadivel ◽  
Jennifer J.P. Collins ◽  
Liqun Xu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Lung Injury ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Induced Pluripotent ◽  
Lung Progenitor

High-throughput screening of human induced pluripotent stem cell-derived brain organoids

2020 ◽  
Vol 335 ◽  
pp. 108627 ◽  
Author(s):  
Madel Durens ◽  
Jonathan Nestor ◽  
Madeline Williams ◽  
Kevin Herold ◽  
Robert F. Niescier ◽  
...  
Keyword(s):  
Stem Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Induced Pluripotent

scholarly journals A high-throughput screening platform for pigment regulating agents using pluripotent stem cell derived melanocytes

2020 ◽  
Author(s):  
Valentin Parat ◽  
Brigitte Onteniente ◽  
Julien Maruotti
Keyword(s):  
Stem Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Light Scatter ◽  
Chemical Treatments ◽  
Human Melanocytes ◽  
Screening Platform ◽  
Induced Pluripotent

AbstractIn this study, we describe a simple and straight-forward assay using induced pluripotent stem cell derived melanocytes and high-throughput flow cytometry, to screen and identify pigment regulating agents. The assays is based on the correlation between forward light-scatter characteristics and melanin content, with pigmented cells displaying high light absorption/low forward light-scatter, while the opposite is true for lowly pigmented melanocytes, as a result of genetic background or chemical treatments. Orthogonal validation is then performed by regular melanin quantification. Such approach was validated using a set of 80 small molecules, and yielded a confirmed hit. The assay described in this study may prove a useful tool to identify modulators of melanogenesis in human melanocytes.

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