Molecular Detection of Dengue Virus in Mosquitoes as an Early Indicator to Aid in the Prevention of Human Infection in Endemic Areas

2020 ◽  
Vol 20 (1) ◽  
pp. 54-59 ◽  
Author(s):  
André de Souza Leandro ◽  
André da Silva Britto ◽  
Jean Avemir Rios ◽  
Sandro Roberto Galvão ◽  
Rosinei Kafka ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Molecular Detection ◽  
Human Infection ◽  
Early Indicator ◽  
Endemic Areas

scholarly journals Triatomine Feeding Profiles and Trypanosoma cruzi Infection, Implications in Domestic and Sylvatic Transmission Cycles in Ecuador

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 42
Author(s):  
Sofía Ocaña-Mayorga ◽  
Juan José Bustillos ◽  
Anita G. Villacís ◽  
C. Miguel Pinto ◽  
Simone Frédérique Brenière ◽  
...  
Keyword(s):  
Blood Meal ◽  
Mitochondrial Gene ◽  
Intestinal Content ◽  
Human Infection ◽  
Dispersal Capacity ◽  
Transmission Cycle ◽  
Rhodnius Ecuadoriensis ◽  
Transmission Cycles ◽  
Endemic Areas ◽  
Southern Highland

Understanding the blood meal patterns of insects that are vectors of diseases is fundamental in unveiling transmission dynamics and developing strategies to impede or decrease human–vector contact. Chagas disease has a complex transmission cycle that implies interactions between vectors, parasites and vertebrate hosts. In Ecuador, limited data on human infection are available; however, the presence of active transmission in endemic areas has been demonstrated. The aim of this study was to determine the diversity of hosts that serve as sources of blood for triatomines in domestic, peridomestic and sylvatic transmission cycles, in two endemic areas of Ecuador (central coastal and southern highland regions). Using conserved primers and DNA extracted from 507 intestinal content samples from five species of triatomines (60 Panstrongylus chinai, 17 Panstrongylus howardi, 1 Panstrongylus rufotuberculatus, 427 Rhodnius ecuadoriensis and 2 Triatoma carrioni) collected from 2006 to 2013, we amplified fragments of the cytb mitochondrial gene. After sequencing, blood meal sources were identified in 416 individuals (146 from central coastal and 270 from southern highland regions), achieving ≥ 95% identity with GenBank sequences (NCBI-BLAST tool). The results showed that humans are the main source of food for triatomines, indicating that human–vector contact is more frequent than previously thought. Although other groups of mammals, such as rodents, are also an available source of blood, birds (particularly chickens) might have a predominant role in the maintenance of triatomines in these areas. However, the diversity of sources of blood found might indicate a preference driven by triatomine species. Moreover, the presence of more than one source of blood in triatomines collected in the same place indicated that dispersal of vectors occurs regardless the availability of food. Dispersal capacity of triatomines needs to be evaluated to propose an effective strategy that limits human–vector contact and, in consequence, to decrease the risk of T. cruzi transmission.

scholarly journals TRANSMISI TRANSOVARIAL VIRUS DENGUE PADA NYAMUKAEDES SPP.

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Angle M. H. Sorisi
Keyword(s):  
Dengue Virus ◽  
Vertical Transmission ◽  
Dengue Hemorrhagic Fever ◽  
Virus Transmission ◽  
Hemorrhagic Fever ◽  
Health Problems ◽  
Transovarial Transmission ◽  
Blood Sucking ◽  
Endemic Areas ◽  
And Control

Abstract: Dengue Hemorrhagic Fever (DHF) is one of the most serious health problems in Indonesia which often causes outbreaks with numerous deaths. The disease is transmitted byAedes sp.females. Generally, dengue virus transmission occurs horizontally from human carriers, and the dengue viruses are passed on bytheir vectors through blood sucking activity. After propagation in the mosquito, the viruses are transmitted to human recipients. In addition, there is a vertical transmission (transovarial) of dengue virusesin the ova of Aedes sp.females. The viruses propagate in the ova that undergo  metamorphosis to become larvae, pupae, and imagoes. The transovarial transmission of dengue virusesin its vectors in endemic areas could be a causative key which is responsible for the phenomenon of increasing cases of DHF. Any effort to prevent and control DHF requires a thorough understanding about virDen transmission, including this transovarial transmission in Aedes spfemales. Keywords: DHF, transovarial transmission, Aedes sp.     Abstrak: Penyakit Demam Berdarah Dengue (DBD) merupakan salah satu masalah kesehatan yang semakin serius di Indonesia dan sering menimbulkan suatu Kejadian Luar Biasa (KLB) dengan jumlah kematian tinggi. Penyakit ditularkan melalui Aedes sp.betina. Transmisi virus dengue umumnya terjadi secara horizontal, yaitu dari manusia pembawa virus dengue ke nyamuk vektor Aedes sp. melalui aktivitasnya mengisap darahSetelah mengalami propagasi  dalam  tubuh nyamuk, virus dengue ditularkan ke  manusia penerima. Selain itu, transmisi virus dapat terjadi secara vertikal (transovarial) yaitu virus dengue dalam tubuh nyamuk vektorAedes sp. betinake ovum, kemudian berpropagasi dalam ovum, larva, pupa, dan imago. Transmisi transovarial virus dengueke vektornya di daerah endemik bisa menjadi kunci penyebab yang bertanggung jawab terhadap fenomena peningkatan kasus deman berdarah dengue. Upaya pencegahan dan penanggulangan DBD memerlukan pengetahuan yang matang tentang adanya infeksi transovarial virDen pada nyamuk Aedes sp. Kata kunci : DBD, transmisi transovarial,  Aedes sp.

Quartz Crystal Microbalance Aptasensor for the Molecular Detection of Dengue Virus

2020 ◽  
Vol MA2020-01 (27) ◽  
pp. 1887-1887
Author(s):  
Geminesse S. Sianghio ◽  
Reyza Kathleen A. Dimaculangan ◽  
Dharmatov Rahula B. Albano
Keyword(s):  
Dengue Virus ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Molecular Detection

scholarly journals Dengue Virus Seroconversion in Travelers to Dengue-Endemic Areas

2016 ◽  
Vol 95 (5) ◽  
pp. 1130-1136 ◽  
Author(s):  
Rosemary M. Olivero ◽  
Emad A. Yanni ◽  
Lin H. Chen ◽  
Laura Kogelman ◽  
Davidson H. Hamer ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Endemic Areas

Preparation of MS2-based nanoparticles as control and standard materials for the molecular detection of dengue virus serotypes

2017 ◽  
Vol 233 ◽  
pp. 42-50 ◽  
Author(s):  
Yu Fu ◽  
Guojing Wang ◽  
Qisheng Wu ◽  
Xin Yang ◽  
Rui Zhang ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Molecular Detection ◽  
Dengue Virus Serotypes

scholarly journals Different serotypes of dengue virus (DENV) imported by Polish travellers from dengue endemic areas to Poland

2015 ◽  
Vol 66 (2) ◽  
pp. 72-76 ◽  
Author(s):  
Beata Biernat ◽  
Joanna Stańczak ◽  
Beata Szostakowska ◽  
Agnieszka Wroczyńska ◽  
Anna Kuna ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Endemic Areas

scholarly journals Prevalence and Molecular Detection of Dengue Virus in 2013 Outbreak in KPK and Punjab, Pakistan

2017 ◽  
Vol 49 (3) ◽  
pp. 1119-1122 ◽  
Author(s):  
Muhammad Shahid ◽  
Iram Amin ◽  
Samia Afzal ◽  
Zareen Fatima ◽  
Sadia Zahid ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Molecular Detection

scholarly journals Brucella Melitensis Review of the Human Infection Case

2006 ◽  
Vol 6 (1) ◽  
pp. 15-18 ◽  
Author(s):  
Šukrija Zvizdić ◽  
Dževad Čengić ◽  
Maja Bratić ◽  
Snježana Mehanić ◽  
Fikret Pinjo ◽  
...  
Keyword(s):  
Brucella Melitensis ◽  
Diagnostic Yield ◽  
Joint Infection ◽  
Human Infection ◽  
Optimal Treatment ◽  
Joint Aspiration ◽  
Joint Infections ◽  
Prosthetic Joint ◽  
Prosthetic Joint Infections ◽  
Endemic Areas

Brucella spp. prosthetic joint infections are infrequently reported in the literature, particularly in returning travellers, and optimal treatment is unknown.METHOD:We describe a prosthetic joint infection (PJI) caused by Brucella melitensis in a traveller returning to the UK from Thailand, which we believe to be the first detailed report of brucellosis in a traveller returning from this area. The 23 patients with Brucella-related PJI reported in the literature are summarised, together with our case.RESULTS:The diagnosis of Brucella-related PJI is difficult to make; only 30% of blood cultures and 75% of joint aspiration cultures were positive in the reported cases. Culture of intraoperative samples provides the best diagnostic yield. In the absence of radiological evidence of joint loosening, combination antimicrobial therapy alone may be appropriate treatment in the first instance; this was successful in 6/7 [86%] of patients, though small numbers of patients and the likelihood of reporting bias warrant caution in drawing any firm conclusions about optimal treatment. Aerosolisation of synovial fluid during joint aspiration procedures and nosocomial infection has been described.CONCLUSIONS:Brucella-related PJI should be considered in the differential of travellers returning from endemic areas with PJI, including Thailand. Personal protective equipment including fit tested filtering face piece-3 (FFP3) mask or equivalent is recommended for personnel carrying out joint aspiration when brucellosis is suspected. Travellers can reduce the risk of brucellosis by avoiding unpasteurised dairy products and animal contact (particularly on farms and abattoirs) in endemic areas and should be counselled regarding these risks as part of their pre-travel assessment.

scholarly journals Molecular Characterization of Imported and Autochthonous Dengue in Northeastern Spain

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1910
Author(s):  
Jessica Navero-Castillejos ◽  
Rosa Benitez ◽  
Nuria Torner ◽  
José Muñoz ◽  
Daniel Camprubí-Ferrer ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Molecular Characterization ◽  
Phylogenetic Analyses ◽  
Genotype I ◽  
Related Case ◽  
Geographical Regions ◽  
Surveillance Programs ◽  
Endemic Areas ◽  
Virus Strains

Dengue is the most significant arbovirus worldwide and a public health threat to non-endemic areas in which Aedes vectors are present. Autochthonous dengue transmission has been reported in several European countries in the last decade. Infected travelers from endemic regions arriving to areas colonized by Aedes albopictus in Europe need to be monitored in surveillance and control programs. We aimed to perform molecular characterization of RT-PCR-positive dengue cases detected in Catalonia, northeastern Spain, from 2013 to 2018. The basic demographic information and the geographical regions of importation were also analyzed. One-hundred four dengue cases were studied (103 imported infections and the first autochthonous case in our region). The dengue virus strains detected were serotyped and genotyped using molecular methods, and phylogenetic analyses were conducted. All four dengue serotypes were detected in travelers, including up to 10 different genotypes, reflecting the global circulation of dengue in endemic areas. The primary travel-related case of the 2018 autochthonous transmission was not identified, but the molecular analysis revealed dengue serotype 1, genotype I of Asian origin. Our results highlight the diversity of imported dengue virus strains and the role of molecular epidemiology in supporting arbovirus surveillance programs.

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