scholarly journals Prevalence and Molecular Detection of Dengue Virus in 2013 Outbreak in KPK and Punjab, Pakistan

2017 ◽  
Vol 49 (3) ◽  
pp. 1119-1122 ◽  
Author(s):  
Muhammad Shahid ◽  
Iram Amin ◽  
Samia Afzal ◽  
Zareen Fatima ◽  
Sadia Zahid ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Molecular Detection

Quartz Crystal Microbalance Aptasensor for the Molecular Detection of Dengue Virus

2020 ◽  
Vol MA2020-01 (27) ◽  
pp. 1887-1887
Author(s):  
Geminesse S. Sianghio ◽  
Reyza Kathleen A. Dimaculangan ◽  
Dharmatov Rahula B. Albano
Keyword(s):  
Dengue Virus ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Molecular Detection

Preparation of MS2-based nanoparticles as control and standard materials for the molecular detection of dengue virus serotypes

2017 ◽  
Vol 233 ◽  
pp. 42-50 ◽  
Author(s):  
Yu Fu ◽  
Guojing Wang ◽  
Qisheng Wu ◽  
Xin Yang ◽  
Rui Zhang ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Molecular Detection ◽  
Dengue Virus Serotypes

scholarly journals Molecular detection of dengue virus in patients suspected of Ebola virus disease in Ghana

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0208907 ◽  
Author(s):  
Joseph Humphrey Kofi Bonney ◽  
Takaya Hayashi ◽  
Samuel Dadzie ◽  
Esinam Agbosu ◽  
Deborah Pratt ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Ebola Virus ◽  
Molecular Detection ◽  
Virus Disease ◽  
Ebola Virus Disease

scholarly journals Molecular detection of mixed infections with multiple dengue virus serotypes in suspected dengue samples in Tamaulipas, Mexico

2017 ◽  
Vol 112 (7) ◽  
pp. 520-522 ◽  
Author(s):  
Rocío Requena-Castro ◽  
Miguel Ángel Reyes-López ◽  
Rosa Eminé Rodríguez-Reyna ◽  
Prisco Palma-Nicolás ◽  
Virgilio Bocanegra-García
Keyword(s):  
Dengue Virus ◽  
Molecular Detection ◽  
Mixed Infections ◽  
Dengue Virus Serotypes

Reverse Transcriptase PCR (Rt-PCR) for Detection of Dengue and Chikungunya Virus of Mosquito Aedes aegypti in Sokaraja

2020 ◽  
Vol 2 (1) ◽  
pp. 56
Author(s):  
Dyah Retno Annisa ◽  
Endang Srimurni Kusmintarsih ◽  
Trisnowati Budi Ambarningrum
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Reverse Transcriptase ◽  
Chikungunya Virus ◽  
Sampling Method ◽  
Molecular Detection ◽  
Transmission Risk ◽  
Virus Infections ◽  
Rt Pcr ◽  
Cross Sectional

dengue fever (DF). Meanwhile, chikungunya virus causes Chikungunya fever (CF). These diseases involve three organisms, namely virus, mosquito Aedes sp., and human. The transmission of dengue and chikungunya virus is related to the population of Ae. aegypti. Banyumas regency is one of the regions with many cases of dengue and chikungnya virus infections, particularly in Purwokerto, Sokaraja, and Cilongok sub-district. Up to this time, there is no medicine and vaccine provided to treat these viruses effectively. Thus, detection of virus inside vector will be effectively performed in order to predict the transmission risk of dengue and chikungunya virus. This research aimed to know the molecular detection of dengue and chikungunya virus on adult Ae.aegypti mosquito in Sokaraja Region, Banyumas Regency. Survey was done by a cross-sectional method in Sokaraja sub-district from May 2019 – March 2019. Furthermore, technical sampling that used was purposive sampling method of adult Ae.aegypti using BG-Sentital Trap, followed by molecular detection of dengue virus using Two-step RT-PCR and chikungunya gene virus using RT-PCR. Molecular detection of DENV and CHIKV of mosquitoes which collected from Sokaraja region showed negative result

Molecular Detection of Dengue Virus in Mosquitoes as an Early Indicator to Aid in the Prevention of Human Infection in Endemic Areas

2020 ◽  
Vol 20 (1) ◽  
pp. 54-59 ◽  
Author(s):  
André de Souza Leandro ◽  
André da Silva Britto ◽  
Jean Avemir Rios ◽  
Sandro Roberto Galvão ◽  
Rosinei Kafka ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Molecular Detection ◽  
Human Infection ◽  
Early Indicator ◽  
Endemic Areas

One-step Multiplex Real-time RT-PCR for Molecular Detection and Typing of Dengue Virus Infection From Paraffin-embedded Tissues During the Brazilian 2019 Outbreak

2020 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Juliana Mariotti Guerra ◽  
Camila Santos da Silva Ferreira ◽  
Karolina Rosa Fernandes Beraldo ◽  
Lidia Midori Kimura ◽  
Juliana Possatto Fernandes Takahashi ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Real Time ◽  
Molecular Detection ◽  
Rt Pcr ◽  
Dengue Virus Infection ◽  
One Step

scholarly journals Serological Estimation, Molecular Detection and Serotyping of Dengue Virus in Dengue Patients of Lahore Region

2021 ◽  
Vol 30 (6) ◽  
pp. 4925-4931
Author(s):  
Nusrat Bano ◽  
Muhammad Tayyab ◽  
Muhammad Pervaiz ◽  
Nosheen Yousaf ◽  
Ali Raza Awan ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Molecular Detection

scholarly journals Co-circulation of dengue virus 1 and 2 several years after single serotype detection in Cross River State, Nigeria

2020 ◽  
Vol 18 (3) ◽  
pp. 167-170
Author(s):  
A.B. Onoja ◽  
U.E. George ◽  
I.S. Gadzama
Keyword(s):  
Dengue Virus ◽  
Molecular Detection ◽  
Rna Extraction ◽  
Hemorrhagic Fever ◽  
Viral Disease ◽  
Entomological Surveillance ◽  
Cross River ◽  
Denv Serotypes ◽  
Cross River State ◽  
Public Health Professionals

Dengue is a mosquito-borne viral disease which affects over 390 million people annually around the world. High rates of unrecognized dengue infections have been observed in Nigeria through serological assays. This informed molecular investigation in some areas where previous reports were made. We screened individuals in a rural community in Nigeria for circulating dengue virus serotypes. Using a community based participatory approach, convenient sampling was employed to collect plasma from 17 febrile adults in Abia and Bendeghe wards within Etung Local Government Area of Cross River State, Nigeria. The area is forested with heavy mosquito presence. RNA extraction was carried out with viral RNA mini kit (QIAamp, QIAGEN GmbH, Hilden, Germany). Molecular detection of DENV serotypes was by Polymerase Chain Reaction using characterized DENV isolates as standard controls. Out of 17 participants, 3 (17.6%) tested positive to DENV. Two were infected with DENV-2 and one with DENV-1. All three were men in Abia ward while no person tested positive to the virus in Bendeghe. This present study highlights co-circulation of DENV-1 which demonstrates a change in the epidemiology of dengue in the area. It reinforces the need for public health professionals in the area to be vigilant, and signals the possibility of an increase in Dengue Hemorrhagic Fever. Sustained entomological surveillance is recommended. Keywords: Dengue, Cross River State, Molecular detection, Nigeria, Rain forest

scholarly journals Improved Molecular Detection of Dengue Virus Serotype 1 Variants

2003 ◽  
Vol 41 (8) ◽  
pp. 3864-3867 ◽  
Author(s):  
J.-M. Reynes ◽  
S. Ong ◽  
C. Mey ◽  
C. Ngan ◽  
S. Hoyer ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Molecular Detection ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Dengue Virus Serotype 1
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