Different serotypes of dengue virus (DENV) imported by Polish travellers from dengue endemic areas to Poland

Abstract: Dengue Hemorrhagic Fever (DHF) is one of the most serious health problems in Indonesia which often causes outbreaks with numerous deaths. The disease is transmitted byAedes sp.females. Generally, dengue virus transmission occurs horizontally from human carriers, and the dengue viruses are passed on bytheir vectors through blood sucking activity. After propagation in the mosquito, the viruses are transmitted to human recipients. In addition, there is a vertical transmission (transovarial) of dengue virusesin the ova of Aedes sp.females. The viruses propagate in the ova that undergo metamorphosis to become larvae, pupae, and imagoes. The transovarial transmission of dengue virusesin its vectors in endemic areas could be a causative key which is responsible for the phenomenon of increasing cases of DHF. Any effort to prevent and control DHF requires a thorough understanding about virDen transmission, including this transovarial transmission in Aedes spfemales. Keywords: DHF, transovarial transmission, Aedes sp. Abstrak: Penyakit Demam Berdarah Dengue (DBD) merupakan salah satu masalah kesehatan yang semakin serius di Indonesia dan sering menimbulkan suatu Kejadian Luar Biasa (KLB) dengan jumlah kematian tinggi. Penyakit ditularkan melalui Aedes sp.betina. Transmisi virus dengue umumnya terjadi secara horizontal, yaitu dari manusia pembawa virus dengue ke nyamuk vektor Aedes sp. melalui aktivitasnya mengisap darahSetelah mengalami propagasi dalam tubuh nyamuk, virus dengue ditularkan ke manusia penerima. Selain itu, transmisi virus dapat terjadi secara vertikal (transovarial) yaitu virus dengue dalam tubuh nyamuk vektorAedes sp. betinake ovum, kemudian berpropagasi dalam ovum, larva, pupa, dan imago. Transmisi transovarial virus dengueke vektornya di daerah endemik bisa menjadi kunci penyebab yang bertanggung jawab terhadap fenomena peningkatan kasus deman berdarah dengue. Upaya pencegahan dan penanggulangan DBD memerlukan pengetahuan yang matang tentang adanya infeksi transovarial virDen pada nyamuk Aedes sp. Kata kunci : DBD, transmisi transovarial, Aedes sp.

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Transovarial transmission of dengue virus on Aedes aegypti atseveral endemic areas of dengue haemorrhagic fever in Indonesia

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/196/1/012040 ◽

2018 ◽

Vol 196 ◽

pp. 012040

Author(s):

U K Hadi ◽

S Soviana ◽

Sugiarto

Keyword(s):

Aedes Aegypti ◽

Dengue Virus ◽

Dengue Haemorrhagic Fever ◽

Transovarial Transmission ◽

Haemorrhagic Fever ◽

Endemic Areas

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Dengue Virus Seroconversion in Travelers to Dengue-Endemic Areas

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.16-0159 ◽

2016 ◽

Vol 95 (5) ◽

pp. 1130-1136 ◽

Cited By ~ 7

Author(s):

Rosemary M. Olivero ◽

Emad A. Yanni ◽

Lin H. Chen ◽

Laura Kogelman ◽

Davidson H. Hamer ◽

...

Keyword(s):

Dengue Virus ◽

Endemic Areas

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Molecular Characterization of Imported and Autochthonous Dengue in Northeastern Spain

Viruses ◽

10.3390/v13101910 ◽

2021 ◽

Vol 13 (10) ◽

pp. 1910

Author(s):

Jessica Navero-Castillejos ◽

Rosa Benitez ◽

Nuria Torner ◽

José Muñoz ◽

Daniel Camprubí-Ferrer ◽

...

Keyword(s):

Dengue Virus ◽

Molecular Characterization ◽

Phylogenetic Analyses ◽

Genotype I ◽

Related Case ◽

Geographical Regions ◽

Surveillance Programs ◽

Endemic Areas ◽

Virus Strains

Dengue is the most significant arbovirus worldwide and a public health threat to non-endemic areas in which Aedes vectors are present. Autochthonous dengue transmission has been reported in several European countries in the last decade. Infected travelers from endemic regions arriving to areas colonized by Aedes albopictus in Europe need to be monitored in surveillance and control programs. We aimed to perform molecular characterization of RT-PCR-positive dengue cases detected in Catalonia, northeastern Spain, from 2013 to 2018. The basic demographic information and the geographical regions of importation were also analyzed. One-hundred four dengue cases were studied (103 imported infections and the first autochthonous case in our region). The dengue virus strains detected were serotyped and genotyped using molecular methods, and phylogenetic analyses were conducted. All four dengue serotypes were detected in travelers, including up to 10 different genotypes, reflecting the global circulation of dengue in endemic areas. The primary travel-related case of the 2018 autochthonous transmission was not identified, but the molecular analysis revealed dengue serotype 1, genotype I of Asian origin. Our results highlight the diversity of imported dengue virus strains and the role of molecular epidemiology in supporting arbovirus surveillance programs.

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CO-INFECTION OF DENGUE VIRUS BY SEROTYPES 1 AND 4 IN PATIENT FROM MEDIUM SIZED CITY FROM BRAZIL

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652013000400009 ◽

2013 ◽

Vol 55 (4) ◽

pp. 275-281 ◽

Cited By ~ 8

Author(s):

Tatiana Elias Colombo ◽

Danila Vedovello ◽

Adriano Mondini ◽

Andrea Francesli Negri Reis ◽

Amena Alcantara Ferras Cury ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Dengue Virus ◽

Human Serum ◽

Molecular Tests ◽

Endemic Areas ◽

Genotype V

SUMMARY The natural co-infection with dengue virus can occur in highly endemic areas where different serotypes have been observed for many years. We report one case of DENV-1/DENV-4 co-infection in human serum detected by molecular tests. Phylogenetic analysis of the sequences obtained indicated the presence of genotype V and II for DENV-1 and DENV-4, respectively.

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Molecular Detection of Dengue Virus in Mosquitoes as an Early Indicator to Aid in the Prevention of Human Infection in Endemic Areas

Vector-Borne and Zoonotic Diseases ◽

10.1089/vbz.2018.2411 ◽

2020 ◽

Vol 20 (1) ◽

pp. 54-59 ◽

Cited By ~ 1

Author(s):

André de Souza Leandro ◽

André da Silva Britto ◽

Jean Avemir Rios ◽

Sandro Roberto Galvão ◽

Rosinei Kafka ◽

...

Keyword(s):

Dengue Virus ◽

Molecular Detection ◽

Human Infection ◽

Early Indicator ◽

Endemic Areas

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Faculty Opinions recommendation of Global Assessment of Dengue Virus-Specific CD4+ T Cell Responses in Dengue-Endemic Areas.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.732052548.793562700 ◽

2019 ◽

Author(s):

Scott Halstead

Keyword(s):

T Cell ◽

Dengue Virus ◽

Global Assessment ◽

T Cell Responses ◽

Cd4 T Cell ◽

Cell Responses ◽

Endemic Areas

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High performance dengue virus antigen-based serotyping-NS1-ELISA (plus): A simple alternative approach to identify dengue virus serotypes in acute dengue specimens

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0009065 ◽

2021 ◽

Vol 15 (2) ◽

pp. e0009065

Author(s):

Tanapan Prommool ◽

Pongpawan Sethanant ◽

Narodom Phaenthaisong ◽

Nattaya Tangthawornchaikul ◽

Adisak Songjaeng ◽

...

Keyword(s):

Dengue Virus ◽

High Performance ◽

Hemorrhagic Fever ◽

Virus Antigen ◽

Enzyme Linked Immunosorbent Assay ◽

Structural Protein ◽

Rt Pcr ◽

Denv Serotypes ◽

Reverse Transcription Pcr ◽

Endemic Areas

Dengue hemorrhagic fever (DHF) is caused by infection with dengue virus (DENV). Four different serotypes (DENV1-4) co-circulate in dengue endemic areas. The viral RNA genome-based reverse-transcription PCR (RT-PCR) is the most widely used method to identify DENV serotypes in patient specimens. However, the non-structural protein 1 (NS1) antigen as a biomarker for DENV serotyping is an emerging alternative method. We modified the serotyping-NS1-enzyme linked immunosorbent assay (stNS1-ELISA) from the originally established assay which had limited sensitivity overall and poor specificity for the DENV2 serotype. Here, four biotinylated serotype-specific antibodies were applied, including an entirely new design for detection of DENV2. Prediction of the infecting serotype of retrospective acute-phase plasma from dengue patients revealed 100% concordance with the standard RT-PCR method for all four serotypes and 78% overall sensitivity (156/200). The sensitivity of DENV1 NS1 detection was greatly improved (from 62% to 90%) by the addition of a DENV1/DENV3 sub-complex antibody pair. Inclusive of five antibody pairs, the stNS1-ELISA (plus) method showed an overall increased sensitivity to 85.5% (171/200). With the same clinical specimens, a commercial NS1 rapid diagnostic test (NS1-RDT) showed 72% sensitivity (147/200), significantly lower than the stNS1-ELISA (plus) performance. In conclusion, the stNS1-ELISA (plus) is an improved method for prediction of DENV serotype and for overall sensitivity. It could be an alternative assay not only for early dengue diagnosis, but also for serotype identification especially in remote resource-limited dengue endemic areas.

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Emergence of dengue in the tribal pockets of Nilgiris district, Tamil Nadu

Tropical Doctor ◽

10.1177/0049475516677158 ◽

2016 ◽

Vol 47 (2) ◽

pp. 170-173

Author(s):

P Philip Samuel ◽

R Govindarajan ◽

S Victor Jerald Leo ◽

R Krishnamoorthy ◽

R Paramasivan ◽

...

Keyword(s):

Dengue Virus ◽

Tamil Nadu ◽

Vector Mosquito ◽

Endemic Areas ◽

Forest Dweller ◽

The Impact

As original tribal ways of living have morphed from a forest dweller existence, dengue is no longer an urban infection but is now also found in rural hilly areas. The spread of dengue is enhanced by the frequent movement of people to endemic areas where there is a vector mosquito presence. The impact of the virus is known to be great in the immunologically naive population. Our study reports on the threat of the dengue virus in these hilly areas.

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