scholarly journals Cornichon-like Protein Facilitates Secretion of HB-EGF and Regulates Proper Development of Cranial Nerves

2007 ◽  
Vol 18 (4) ◽  
pp. 1143-1152 ◽  
Author(s):  
Hideharu Hoshino ◽  
Tsukasa Uchida ◽  
Toshiaki Otsuki ◽  
Shoko Kawamoto ◽  
Kousaku Okubo ◽  
...  

During their migration to the periphery, cranial neural crest cells (NCCs) are repulsed by an ErbB4-dependent cue(s) in the mesenchyme adjoining rhombomeres (r) 3 and 5, which are segmented hindbrain neuromeres. ErbB4 has many ligands, but which ligand functions in the above system has not yet been clearly determined. Here we found that a cornichon-like protein/cornichon homolog 2 (CNIL/CNIH2) gene was expressed in the developing chick r3 and r5. In a cell culture system, its product facilitated the secretion of heparin-binding epidermal growth factor-like growth factor (HB-EGF), one of the ligands of ErbB4. When CNIL function was perturbed in chick embryos by forced expression of a truncated form of CNIL, the distribution of NCCs was affected, which resulted in abnormal nerve fiber connections among the cranial sensory ganglia. Also, knockdown of CNIL or HB-EGF with siRNAs yielded a similar phenotype. This phenotype closely resembled that of ErbB4 knockout mouse embryos. Because HB-EGF was uniformly expressed in the embryonic hindbrain, CNIL seems to confine the site of HB-EGF action to r3 and r5 in concert with ErbB4.

1994 ◽  
Vol 269 (31) ◽  
pp. 20060-20066 ◽  
Author(s):  
K. Hashimoto ◽  
S. Higashiyama ◽  
H. Asada ◽  
E. Hashimura ◽  
T. Kobayashi ◽  
...  

2004 ◽  
Vol 18 (8) ◽  
pp. 2035-2048 ◽  
Author(s):  
Bukhtiar H. Shah ◽  
Akin Yesilkaya ◽  
J. Alberto Olivares-Reyes ◽  
Hung-Dar Chen ◽  
László Hunyady ◽  
...  

1997 ◽  
Vol 272 (22) ◽  
pp. 14349-14355 ◽  
Author(s):  
Eiji Miyoshi ◽  
Shigeki Higashiyama ◽  
Takatoshi Nakagawa ◽  
Norio Hayashi ◽  
Naoyuki Taniguchi

Sign in / Sign up

Export Citation Format

Share Document