scholarly journals Live-cell monitoring of periodic gene expression in synchronous human cells identifies Forkhead genes involved in cell cycle control

2012 ◽  
Vol 23 (16) ◽  
pp. 3079-3093 ◽  
Author(s):  
Gavin D. Grant ◽  
Joshua Gamsby ◽  
Viktor Martyanov ◽  
Lionel Brooks ◽  
Lacy K. George ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Dna Binding ◽  
Luciferase Activity ◽  
Binding Motif ◽  
Sequencing Analysis ◽  
Peak Expression ◽  
Periodic Gene ◽  
Regulated Gene Expression ◽  
Periodic Gene Expression

We developed a system to monitor periodic luciferase activity from cell cycle–regulated promoters in synchronous cells. Reporters were driven by a minimal human E2F1 promoter with peak expression in G1/S or a basal promoter with six Forkhead DNA-binding sites with peak expression at G2/M. After cell cycle synchronization, luciferase activity was measured in live cells at 10-min intervals across three to four synchronous cell cycles, allowing unprecedented resolution of cell cycle–regulated gene expression. We used this assay to screen Forkhead transcription factors for control of periodic gene expression. We confirmed a role for FOXM1 and identified two novel cell cycle regulators, FOXJ3 and FOXK1. Knockdown of FOXJ3 and FOXK1 eliminated cell cycle–dependent oscillations and resulted in decreased cell proliferation rates. Analysis of genes regulated by FOXJ3 and FOXK1 showed that FOXJ3 may regulate a network of zinc finger proteins and that FOXK1 binds to the promoter and regulates DHFR, TYMS, GSDMD, and the E2F binding partner TFDP1. Chromatin immunoprecipitation followed by high-throughput sequencing analysis identified 4329 genomic loci bound by FOXK1, 83% of which contained a FOXK1-binding motif. We verified that a subset of these loci are activated by wild-type FOXK1 but not by a FOXK1 (H355A) DNA-binding mutant.

scholarly journals Cell Cycle Programs of Gene Expression Control Morphogenetic Protein Localization

2000 ◽  
Vol 151 (7) ◽  
pp. 1501-1512 ◽  
Author(s):  
Matthew Lord ◽  
Melody C. Yang ◽  
Michelle Mischke ◽  
John Chant
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Protein Localization ◽  
Expression Profiles ◽  
Direct Role ◽  
Periodic Gene ◽  
Gene Expression Control ◽  
Genomic Studies ◽  
And Function ◽  
Periodic Gene Expression

Genomic studies in yeast have revealed that one eighth of genes are cell cycle regulated in their expression. Almost without exception, the significance of cell cycle periodic gene expression has not been tested. Given that many such genes are critical to cellular morphogenesis, we wanted to examine the importance of periodic gene expression to this process. The expression profiles of two genes required for the axial pattern of cell division, BUD3 and BUD10/AXL2/SRO4, are strongly cell cycle regulated. BUD3 is expressed close to the onset of mitosis. BUD10 is expressed in late G1. Through promotor-swap experiments, the expression profile of each gene was altered and the consequences examined. We found that an S/G2 pulse of BUD3 expression controls the timing of Bud3p localization, but that this timing is not critical to Bud3p function. In contrast, a G1 pulse of BUD10 expression plays a direct role in Bud10p localization and function. Bud10p, a membrane protein, relies on the polarized secretory machinery specific to G1 to be delivered to its proper location. Such a secretion-based targeting mechanism for membrane proteins provides cells with flexibility in remodeling their architecture or evolving new forms.

Periodic gene expression program of the fission yeast cell cycle

2004 ◽  
Vol 36 (8) ◽  
pp. 809-817 ◽  
Author(s):  
Gabriella Rustici ◽  
Juan Mata ◽  
Katja Kivinen ◽  
Pietro Lió ◽  
Christopher J Penkett ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Yeast Cell ◽  
Fission Yeast ◽  
Gene Expression Program ◽  
Yeast Cell Cycle ◽  
Fission Yeast Cell ◽  
Periodic Gene ◽  
Expression Program ◽  
Periodic Gene Expression

scholarly journals Oscillatory Phosphorylation of Yeast Fus3 MAP Kinase Controls Periodic Gene Expression and Morphogenesis

2008 ◽  
Vol 18 (23) ◽  
pp. 1897
Author(s):  
Zoe Hilioti ◽  
Walid Sabbagh ◽  
Saurabh Paliwal ◽  
Adriel Bergmann ◽  
Marcus D. Goncalves ◽  
...  
Keyword(s):  
Gene Expression ◽  
Map Kinase ◽  
Periodic Gene ◽  
Periodic Gene Expression

scholarly journals Cell Cycle Localization, Dimerization, and Binding Domain Architecture of the Telomere Protein cPot1

2004 ◽  
Vol 24 (5) ◽  
pp. 2091-2102 ◽  
Author(s):  
Chao Wei ◽  
Carolyn M. Price
Keyword(s):  
Cell Cycle ◽  
Dna Binding ◽  
Binding Site ◽  
Domain Architecture ◽  
Binding Domain ◽  
Binding Motif ◽  
Dna Binding Domain ◽  
Telomere Protein ◽  
Ob Fold

ABSTRACT Pot1 is a single-stranded-DNA-binding protein that recognizes telomeric G-strand DNA. It is essential for telomere capping in Saccharomyces pombe and regulates telomere length in humans. Human Pot1 also interacts with proteins that bind the duplex region of the telomeric tract. Thus, like Cdc13 from S. cerevisiae, Pot 1 may have multiple roles at the telomere. We show here that endogenous chicken Pot1 (cPot1) is present at telomeres during periods of the cell cycle when t loops are thought to be present. Since cPot1 can bind internal loops and directly adjacent DNA-binding sites, it is likely to fully coat and protect both G-strand overhangs and the displaced G strand of a t loop. The minimum binding site of cPot1 is double that of the S. pombe DNA-binding domain. Although cPot can self associate, dimerization is not required for DNA binding and hence does not explain the binding-site duplication. Instead, the DNA-binding domain appears to be extended to contain a second binding motif in addition to the conserved oligonucleotide-oligosaccharide (OB) fold present in other G-strand-binding proteins. This second motif could be another OB fold. Although dimerization is inefficient in vitro, it may be regulated in vivo and could promote association with other telomere proteins and/or telomere compaction.

Requirement for prolactin during cell cycle regulated gene expression in cloned T-lymphocytes.

Endocrinology ◽  
1992 ◽  
Vol 130 (6) ◽  
pp. 3216-3222 ◽  
Author(s):  
C V Clevenger ◽  
A L Sillman ◽  
J Hanley-Hyde ◽  
M B Prystowsky
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
T Lymphocytes ◽  
Regulated Gene Expression
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