Application of Phage-displayed Single Chain Antibodies in Western Blot
2005 ◽
Vol 37
(3)
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pp. 205-209
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Keyword(s):
Abstract A phage display single chain fragment variable library constructed on pIII protein of M13 filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage-ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody-displayed phage particles and antigens.
2017 ◽
Vol 18
(10)
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pp. 2064
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2019 ◽
Vol 124
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pp. 17-24
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2008 ◽
Vol 58
(1)
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pp. 140-147
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