The interaction between the pump and pump, the signal and signal ,the pump and signal in Fiber Raman amplifiers are measured. Three-wavelength pumping at 1427nm, 1445nm and 1466nm, it show that the pump energy transfers from short wavelength to long wavelength, that is, Raman effect exists between the different pumping lights. When the different wavelength signals input at the same time, the output optical power change, which is also caused due to the Raman interactions between different signals. When three-wavelength pumping, the input signal power, respectively 0.03dBm 5.04dBm 15.08dBm, the signal gain values are measured. A significant gain saturation phenomenon in 15.08 dBm appears, and it will intensify as the power increases.