EFFICIENCY OF DIFFERENT ASSESSMENT METHODS OF BREEDING BULLS OF BLACK-AND-WHITE BREED BY THE OFFSPRING QUALITY

The paper presents results of assessing black-and-white bulls by the quality of the offspring with application of different methods. The evaluation of bulls was carried out with application of the “daughter-mother” method in the conditions of Volzhsky Research Institute of Hydraulic Engineering and Land Reclamation. The valuation showed that the bulls Aist 7 and Muscat 8 increased milk yield of daughters, in comparison with mothers, by 951 and 486 kg, or by 26.7 and 12.9%. The rest of the servicing bulls reduced milk yield of the offspring by 46 - 1019 kg (1.1 - 24.7%). When assessing the bulls using the “daughterpeer” method, the highest milk productivity was shown by the descendants of Sazan 25 and Aist 7 of Vis Back Ideal line, which surpassed their peers in milk yield by 273 and 419 kg, or by 3.8 and 5.7%, respectively. The producers Snop 57 (his 17 daughters had average milk yield of 4224 kg for the first lactation with milk fat of 3.80%) and Muscat 8 (with an average milk yield of daughters of 4254 kg , milk fat - 3.83%) are also of significant value for the breeding work of this farm. According to the assessment results, the producer Aist 7 was assigned to the breeding category of A1 of milk yield improvers. Bulls Sazan 25 and Muscat 8 are also rated as daughters’ milk yield improvers and are categorized as A2 and A3. All bulls evaluated by comparing daughters to the breed standards are improvers of daughters’ milk yield (+ 454-1860 kg).

ZEOLITES AND FERTILIZERS ON THEIR BASIS IN SOYBEAN CULTIVATION TECHNOLOGY IN THE VOLGA FOREST STEPPE

Experimental field studies were carried out on the experimental field of Ulyanovsk State Agrarian University in 2018-2020. The experiment scheme in 2019 (laid in 2018) consisted of 4 variants: 1. Control (without fertilizers); 2. Zeolite in pure form, 500 kg / ha; 3. Organic mineral fertilizer, obtained by enriching zeolites with amino acids, 500 kg / ha; 4. Zeolite 500 kg / ha + N40 (carbamide at the dose of 40 kg of nitrogen per 1 ha). In 2020, the experiment was supplemented with four variants in order to identify more appropriate doses of fertilization (zeolite enriched with amino acids, 250 kg / ha; zeolite enriched with amino acids, 500 kg / ha; zeolite enriched with carbamide, 250 kg / ha; zeolite enriched with urea , 500 kg / ha). The experiments were carried out in 3-fold repetition with a randomized placement of plots. The effect of zeolite from Yushansk deposit of Ulyanovsk region and fertilizers based on it enriched with amino acids and urea were studied in the experiment, as well as their effect on general biological activity of leached black soil, on its agrochemical parameters, photosynthetic activity of soybean crops, yield and seed quality. A significant influence of experimental fertilizers on biological activity of the soil, improvement of nutrient regime, as well as the ability of zeolite to neutralize soil acidity was found. Improvement of the soil environment in case of application of zeolite enriched with amino acids and urea as a fertilizer contributed to an increase of soybean yields by 6-14% and by 12-31% to improvement of the product quality. With an increase of the dose of fertilizers, the yield of soybean seeds increased, but not proportionally.

YIELD OF PERENNIAL GRASSES AND SOIL FERTILITY OF SOD-PODZOLIC SOIL IN CASE OF LONG-TERM APPLICATION OF MINERAL FERTILIZERS

The article considers the effect of increasing doses and various combinations of mineral fertilizers applied to the cover crop on the yield and quality of a mixture of perennial grasses of two - years use, as well as the effect of fertilizers on the agrochemical parameters of sod-podzolic light loamy soil. The objects of the study were Stodolich meadow clover and Leningradskaya 204 meadow timothy grass. The studies were carried out in two crop rotations - with standard and reduced single doses of mineral fertilizers. The research results showed that in the years (VIII rotation) when the unit dose of fertilizers was increased to N20P20K25, the yield of perennial grasses was higher compared to the harvest in the VII rotation. The most appropriate dose in the VII rotation on perennial grasses of the 1st year was N30P30K45, on grasses of the 2nd year - doses of N30P30K45 and N40P40K60. In the VIII rotation, the highest yield of perennial grasses of the 1st year of use was obtained at a dose of N160P160K200 (8.4 t / ha), of grasses of the 2nd year of use at doses of N80P80K100 and N100P100K125 (6.5 t / ha). Positive effect of increasing doses of mineral fertilizers on the content of soil organic matter has been noted. On the contrary, the soil acidity increased from 4.7 to 4.3 units with an increase of mineral doses. Higher doses of fertilizers increased the content of mobile forms of phosphorus and potassium in the soil.

NEW SOURCES OF SEEDLESS GRAPES OF ANAPA EXPERIMENTAL STATION SELECTION

It is commonly known that the share of seedless grape varieties in the total production in the country is very small. Of 106 table varieties included in the register, only 4 varieties are of kishmish direction (Kishmish radiant, Korinka Russian, Yuzhnoberezhny, Yalta seedless). These varieties are in great demand, since their berries are used both fresh and dried. This article presents results of long-term research on isolation of genetic seedlessness donors among grape varieties of Anapa zonal experimental station of viticulture and winemaking, such as Lotos, Zhemchug of Anapa and Kishmish pink AZOS. These studies were carried out with a simultaneous complex study of economically valuable traits and annually changing weather conditions. In addition, the assessment of these varieties was carried out according to seedlessness sign intensity in the conditions of the Anapo-Taman viticulture zone. The greatest mass of rudiments of all analyzed samples was found in Kishmish pink AZOS variety, therefore, it was assigned to the third category of seedlessness, although the mass ratio of rudiments to the mass of the berry is negligible. The rest of the studied seedless grape varieties in comparison with the control variety (Radiant Kishmish) have a higher seedless class. It particularly concerns varieties such varieties as Zhemchug of Anapa and Lotus, belonging to the first and second classes of seedlessness, respectively. Along with this, a long-term work was carried out to study the yield of these varieties and their productivity (coefficients of fruiting and fertility), the phenology of plant development depending on weather conditions. In particular, it was found that high productivity parameters are observed in all the studied varieties every year; Lotus and Zhemchug of Anapa have also high yield every year. The conducted work has shown prospects for using grape varieties of Anapa Experimental Station as future seedlessness donors.

DEVELOPMENT OF ISOLATION SCHEME AND BACTERIOLOGICAL IDENTIFICATION OF PSEUDOMONAS SYRINGAE BACTERIA AND ITS APPROBATION

The article presents results of studies on development of isolation scheme and bacteriological identification of Pseudomonas syringae bacteria and its approbation. The introduction of the article describes the objects of Pseudomonas syringae contamination - fruit trees and shrubs, agricultural plants, which proves the relevance of the research in the field of expanding of laboratory methods for identifying phytopathogenic microorganisms. The author’s bacteriological scheme includes the use of King B Medium (Pseudomonas F Agar; Pronadisa 1532) as a selective medium. Initially, the isolated bacteria are differentiated to Pseudomonas genus, the following paramters are studied: anaerobic fermentation, production of enzymes catalase, lecithinase, lipase; hydrolysis of starch and gelatin; fermentation of glucose and lactose, also, a test for maceration is put. The second stage of the research includes the study of the growth of bacterial cultures on meat-and-peptone agar at 41 ° C and at 5% of NaCl; oxidase production, arginine hydrolase; fermentation of mannose and sorbitol; formation of levan, hydrogen sulfide and indole, esculin, a hypersensitivity reaction is set. The determined parametres allow to type the representatives of the genus to Pseudomonas syringae species within 192 hours. During the research, a collection of 12 strains of Pseudomonas syringae bacteria was formed, isolated from 97 objects of phytosanitary supervision and identified according to the developed technique. The proposed bacteriological scheme allows to differentiate the above microorganisms on the basis of the analysis of 25 parameters. The application of a phage biological product as a diagnosticum (according to the Otto method) expands the spectrum of the analyzed biological properties of the isolated and identified Pseudomonas syringae bacteria.

USAGE OF METABOLISM ENERGY IN FORMATION OF BODY THERMAL STATE OF THE CATTLE

Scientific advances in biological sciences make it possible to significantly increase the energy efficiency of productive livestock. For life, as the highest form of existence of matter, thermal energy is of particular importance. It does not only connect the actions and interactions of all types of matter, it creates order from the chaotic movements of discrete heat sources, determining the measure of irreversible energy dissipation (entropy) and the change gradient of metabolic processes, “outflow and inflow of energy”, the state of saturation and deficiency of nutrients in the body. Metabolic energy is the energy of nutrients entering the tissues and cells of the body from the digestive tract. In the process of intracellular metabolism, substances are converted into new compounds, energy is released and accumulated. Approximately half of the energy is used in the electrochemical reactions of the synthesis of substances inherent in this organism. Heredity, age, environment, condition of animals influence their quantity and quality. The second half of the energy generated in the basic metabolism is “dissipated” and released into the internal and external environment. This part of the energy, in the thermoregulation process, provides isothermal state of the body of animals. Thermal homeostasis, the range of fluctuations in body temperature within the physiological norm is a significant part of the metabolic energy consumption. The article presents results of studying such consumptions when adapting to feeding factors and changes of weather conditions of cattle of different age and productivity.

THE EXPERIENCE OF JOINT USAGE OF SORBENTS AND PROBIOTICS IN POULTY BREEDING

The studies were carried out in the conditions of ZAO PPF “Kavkaz” of the Krasnodar Territory. The article presents results of studies on separate and combined usage of active coal feed additive (ACFA) as a sorbent and “Sporothermin” probiotic for development of muscles and internal organs of broiler chickens of “Cobb-500” cross. The studies were carried out in accordance with the “Methodology for conducting scientific and industrial research on agricultural poultry.” At the end of the experimental period, the development of the muscles and internal organs of the bird was studied. In the course of the experiment, the survivability of the bird, the increase of live weight, and the cost of feed were also determined. As a result of the studies, it was revealed that the use of a probiotic and a sorbent increases the live weight of an un-gutted carcass by 5.0-8.7%. With the combined application of a sorbent and a probiotic, the mass of the glandular stomach was significantly increased by 0.15 abs. % (P <0.001). Intestinal mass significantly grew up with application of ACFA sorbent by 0.42 abs. % (p <0.05). Intestinal length evidently decreased by 8.5% (p <0.001) when using “Sporothermine” probiotic. It should be noted that the mass of the thigh muscles significantly increased by 29.0% (p <0.05) in the second experimental group with usage of ACFA, in relation to the control. At the end of the experiment, the live weight of poultry increased by 4.2% (p <0.01) in the second group with ACFA application. In the third group, where “Sporothermin” was used, the live weight grew up by 9.0% (p <0.001). In the fourth experimental group, which was fed with both the sorbent and the probiotic, the best results were obtained and the live weight significantly increased by 9.7% (p <0.001). Over the entire experiment period, feed costs per unit of production have been reduced by 4.3- 8.6%. The survivability of the poultry population was 94.4% in the control. In the second and third groups, the survival rate was also high and exceeded the control by 3.0%, in the fourth - by 5.6%.

WHEAT RUST IDENTIFICATION USING PHYTOPATHOLOGICAL RESEARCH AND MACHINE VISION TECHNOLOGIES

Wheat is the most economically important and valuable food crop cultivated in most regions of the world, and various diseases have a significant impact on yield parameters. Particular attention in wheat protection technologies from phytopathogens is given to rust, since yield losses, depending on the weather conditions of the season and the resistance of the sown varieties, can range from 30 to 100%. The article provides brief information on wheat rust diseases (yellow, brown, stem rust), as well as on current methods of their identification. Accurate and timely identification of rust pathogens is a key step in making decisions on application of plant protection products in the battle against diseases, which prevents their further development, spread and the occurrence of epiphytoties. The article describes the main method for identification and further record of yellow, brown, stem rust - this is a classic phytopathological study based on usage of human resources. The advantage of this method is its accuracy and versatility. Among the drawbacks, one should single out the labor intensity and the need for a staff of qualified phytopathologists. In view of intensive development of computer technologies and agriculture digitalization, the possibility of using machine vision based on programming of neural networks and their training in identifying the main causative agents of diseases is acquiring scientific and practical interest. A promising methodological approach to identification of phytopathogens when providing phytosanitary monitoring and algorithms used for training of neural networks and applied in machine vision technologies are presented.

EXPERIENCE OF SELECTION AND BREEDING WORK WITH CATTLE OF RED-SPOTTED BREED

The article presents assessment results of red-and-white breed lines on milk productivity, live weight and intensity of milk yield. It was found that almost all the descendants of the bulls belonging to the lines of V.B. Ideal 1014315, M. Chieftain 95679, R. Sovering 198998, R. Citation 267150 are characterized by high milk yield. The only exception was the daughters of the bulls from R. Sovering 198998 line, who were inferior to their peers in milk yield by 225-250 kg (4.1-4.5%) during 305 - day lactation. There were no significant interline differences in the content of fat and protein in milk. It was found that positive results were obtained from crosses of V.B. Ideal × M. Chieftain (+204 kg of milk), M. Chieftain × V.B. Ideal (+300 kg), M. Chieftain × R. Sovering (+185 kg), R. Sovering × R. Sheilimar. Intraline selection on milk yield in all cases was ineffective. Assessment of servicing bulls of V.B. Ideal line showed that the improvers of the daughters’ milk yield are the bulls Kumir 1242 (+283 kg to their peers), Leonardo 218 (+244 kg), Jul 43 (+143 kg). Bulls Clemens 12700 (- 201 kg) and Furgon 1268 (- 679 kg) were recognized as deteriorating the milk yield. As for R. Sovering line, the bulls Service 101 (+403 kg) and Mills 284 (+263 kg) are the milk yield improvers, and the bull Berkut 3473 (+0.38%) improves fat content of milk of the daughters.

ESTIMATION OF RELIABILITY OF CLINICAL STUDY RESULT OF BOVHYALURONIDASE AZOXYMER MEDICATION APPLIED TO CATS WITH UROLOGICAL DISEASES

The article interprets the results of a randomized, blind, placebo-controlled, confirmatory clinical study of the efficacy of bovhyaluronidase azoxymer (BA) applied to cats with urological diseases, conducted in parallel groups on the basis of “Bars” veterinary clinics in Petersburg and the Department of Obstetrics and Operative Surgery of St. Petersburg State University of Veterinary Medicine from November 2018 to August 2020. To prove the causal relationship between the application of BA and a significant decrease of the number of complications in the postoperative period, we used a three-stage system for analyzing the results. The reliability of our study is confirmed by the following: 1) a sufficient sample number corresponding to that calculated at the planning stage of the clinical study (53 cats: 24 were included in the experimental group and 29 were in the control group) that had indications for surgery on the urethra and / or bladder; 2) a sufficient value of the confirmed effect: when BA was used, the risk of complications was 80.9% lower, and the chance of complications decreased by 12.2 times; 3) correct randomization and blinding, which made it possible to obtain representative samples: block randomization and blinding by territory were carried out; 4) standardization of animal registration and data collection: all animals were examined in accordance with the requirements of GOST R 58090-2018 “Clinical examination of nonproductive animals”; 5) the presence of placebo control. The revealed decrease of the frequency of complications with BA is explained by the action mechanism of the studied medication in the body. The results of clinical studies indicate that application of BA medication in the therapy of companion animals is clinically justified and can be extended beyond the observed population. The obtained results allow us to assert that application of BA significantly reduces the complications associated with the performed surgical intervention or the recurrence of the disease within six months after the first operation.