6. Manufacturing and maintaining DNA

Dna Replication ◽

Dna Sequencing ◽

Double Helix ◽

Complementary Strand ◽

Chain Reaction ◽

Parent Molecule ◽

Polymerase Chain ◽

Dna Double Helix ◽

Shed Light

This chapter assesses DNA replication. The double-helix of DNA could easily be separated into two individual strands, each of which acts as a template for the manufacturing of a new complementary strand. This became known as semi-conservative replication, to indicate that each daughter DNA double-helix contained one new strand and one old strand, conserved from the parent molecule. Discovering the ways by which cells replicate their DNA led to the question: can we harness these amazing natural machines to shed light on DNA itself? The chapter then examines two key biotechnological advances: DNA sequencing and the polymerase chain reaction (PCR).

Direct DNA Sequencing of Complementary DNA Amplified by the Polymerase Chain Reaction

Protocols in Human Molecular Genetics ◽

10.1385/0-89603-205-1:9 ◽

2003 ◽

pp. 9-20

Author(s):

Richard A. Gibbs ◽

Phi-Nga Nguyen ◽

C. Thomas Caskey

Keyword(s):

Dna Sequencing ◽

Chain Reaction ◽

Complementary Dna ◽

Direct Dna Sequencing ◽

Linear amplification DNA sequencing (linear polymerase chain reaction sequencing; double-stranded DNA cycle sequencing; cycle sequencing)

The Dictionary of Genomics, Transcriptomics and Proteomics ◽

10.1002/9783527678679.dg06829 ◽

2015 ◽

pp. 1-1

Keyword(s):

Dna Sequencing ◽

Chain Reaction ◽

Linear Amplification ◽

Cycle Sequencing ◽

Double Stranded Dna ◽

Polymerase Chain ◽

Reaction Sequencing

Toxoplasmic encephalitis of basal ganglia with tumor‐like features proven by pathogen‐specific polymerase chain reaction and direct DNA sequencing

Neuropathology ◽

10.1111/neup.12588 ◽

2019 ◽

Vol 39 (5) ◽

pp. 398-403

Author(s):

Yunyun Li ◽

Zhi Zhu ◽

Jianjun Wang

Keyword(s):

Basal Ganglia ◽

Dna Sequencing ◽

Toxoplasmic Encephalitis ◽

Chain Reaction ◽

Direct Dna Sequencing ◽

Specific Polymerase Chain Reaction ◽

Polymerase Chain Reaction and DNA Sequencing for Detection of Ovine Herpesvirus 2 in American Bison (Bison Bison)

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063870201400108 ◽

2002 ◽

Vol 14 (1) ◽

pp. 40-46 ◽

Cited By ~ 5

Author(s):

Elizabeth A. Sausker ◽

Neil W. Dyer

Keyword(s):

Dna Sequencing ◽

Bison Bison ◽

Chain Reaction ◽

Newer Emerging Pathogens of Ocular Non-Sporulating Molds (NSM) Identified by Polymerase Chain Reaction (PCR)-Based DNA Sequencing Technique Targeting Internal Transcribed Spacer (ITS) Region

Current Eye Research ◽

10.1080/02713680701864780 ◽

2008 ◽

Vol 33 (2) ◽

pp. 139-147 ◽

Cited By ~ 35

Author(s):

R. Bagyalakshmi ◽

K. L. Therese ◽

S. Prasanna ◽

H. N. Madhavan

Keyword(s):

Dna Sequencing ◽

Internal Transcribed Spacer ◽

Its Region ◽

Emerging Pathogens ◽

Chain Reaction ◽

Sequencing Technique ◽

Use of the Polymerase Chain Reaction and DNA Sequencing for Detection of Bartonella quintana in the Aortic Valve of a Patient with Culture-Negative Infective Endocarditis

Clinical Infectious Diseases ◽

10.1093/clinids/21.4.891 ◽

1995 ◽

Vol 21 (4) ◽

pp. 891-896 ◽

Cited By ~ 61

Author(s):

J. Jalava ◽

P. Kotilainen ◽

S. Nikkari ◽

M. Skurnik ◽

E. Vanttinen ◽

...

Keyword(s):

Infective Endocarditis ◽

Aortic Valve ◽

Dna Sequencing ◽

Chain Reaction ◽

Bartonella Quintana ◽

Polymerase Chain ◽

Culture Negative

Optimisation and analysis of polymerase chain reaction based DNA sequencing for genotyping polyoma virus in renal transplant patients: A report from South India

Indian Journal of Medical Microbiology ◽

10.4103/0255-0857.150878 ◽

2015 ◽

Vol 33 (5) ◽

pp. 37 ◽

Cited By ~ 2

Author(s):

HN Madhavan ◽

R Bagyalakshmi ◽

M Revathy ◽

P Aarthi ◽

J Malathi

Keyword(s):

Renal Transplant ◽

Dna Sequencing ◽

South India ◽

Polyoma Virus ◽

Chain Reaction ◽

Transplant Patients ◽

Polymerase Chain ◽

Renal Transplant Patients

Polymerase chain reaction-directed DNA sequencing of bleomycin-induced “nondeletion”-Type, 6-thioguanine-resistant mutants in chinese hamster ovary cell derivative AS52: Effects of an inhibitor and a mimic of superoxide dismutase

Environmental and Molecular Mutagenesis ◽

10.1002/em.2850230205 ◽

1994 ◽

Vol 23 (2) ◽

pp. 101-109 ◽

Cited By ~ 13

Author(s):

Jie An ◽

Abraham W. Hsie

Keyword(s):

Superoxide Dismutase ◽

Dna Sequencing ◽

Chinese Hamster Ovary Cell ◽

Chinese Hamster Ovary ◽

Chinese Hamster ◽

Ovary Cell ◽

Chain Reaction ◽

Polymerase Chain ◽

Resistant Mutants

Analysis of p53 Gene Mutations in Human Gliomas by Polymerase Chain Reaction-Based Single-Strand Conformation Polymorphism and DNA Sequencing

Diagnostic Molecular Pathology ◽

10.1097/00019606-199403010-00002 ◽

1994 ◽

Vol 3 (1) ◽

pp. 2-8 ◽

Cited By ~ 17

Author(s):

F. H. Sarkar ◽

W. J. Kupsky ◽

Y.-W. Li ◽

P. Sreepathi

Keyword(s):

Dna Sequencing ◽

Gene Mutations ◽

P53 Gene ◽

Single Strand Conformation Polymorphism ◽

Single Strand ◽

Human Gliomas ◽

Chain Reaction ◽

Polymerase Chain ◽

Conformation Polymorphism

RANCANGAN PRIMER SPESIFIK GEN MACROPHAGE MANNOSE RECEPTOR (MMR) UNTUK POLYMERASE CHAIN REACTION (PCR) DAN SEKUENSING DEOXYRIBO NUCLEIC ACID (DNA)

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY ◽

10.24293/ijcpml.v22i2.1120 ◽

2018 ◽

Vol 22 (2) ◽

pp. 158

Author(s):

Yani Triyani ◽

Nurizzatun Nafsi ◽

Lelly Yuniarti ◽

Nanan Sekarwana ◽

Endang Sutedja ◽

...

Keyword(s):

Dna Sequencing ◽

Mannose Receptor ◽

Primer Design ◽

Sequencing Analysis ◽

Nucleotide Polymorphisms ◽

Chain Reaction ◽

Specific Pcr ◽

Polymerase Chain ◽

Dna Sequencing Analysis

The order (sequencing) determinationof DeoxyribonucleicAcid (DNA) bases is the gene’s most basic information, using the methodof Polymerase Chain Reaction (PCR) as its stage. A key factor of successful detection by PCR is specific PCR primer design choice. Thedetection of diversity of Mycobacterium Mannose Receptor (MMR) gene, responsible for recognizing mannosylated antigen structureof Mycobacterium tuberculosis (M.tb) by DNA sequencing of exon 7 chromosome 10p12, related to susceptiblity for PulmonaryTuberculosis(TB), was first performed in China in 2012. The purpose of this study was to find specific primerfromboth design originatedfrom the research in China/primer I and my own design/primer IIby using Primer3 software. This study was based on 10 healthy subjectsand was a preliminary study of a research titled. The Relationship of Single Nucleotide Polymorphisms (SNPs) of Macrophage MannoseReceptor Gene to Pulmonary Tuberculosis Cases. The examination materials consist of 3 mL of EDTA blood and DNA extraction from itsbuffy coat. The resulting DNA was processed by PCR to amplify MMR gene with primer I and II. The primer I successfully amplified DNAfragments up to 780bp while primer II only 329 bp. The MMR gene DNA sequencing analysis was performed on the amplification resultof both kinds primers by using DNA Baser and Ensembl−BLAST software. The results were different, DNA sequencing result by using theprimer I was found in several chromosomes and also in several loci. Whereas, by using the primer II, it was only found in chromosome10 and in the same locus. Based on this study, it can be concluded that the specific primer design is one of the most important factorsin the success of DNA sequencing.