Lipoproteins and cholesterol esterification rate in plasma during a 10-day modified fast in man

1980 ◽  
Vol 33 (9) ◽  
pp. 1925-1931 ◽  
Author(s):  
L Wallentin ◽  
L Sköldstam
Keyword(s):  
Cholesterol Esterification ◽  
Esterification Rate

In vitro cholesterol esterification in human serum.

1977 ◽  
Vol 23 (3) ◽  
pp. 447-453 ◽  
Author(s):  
J K Yao ◽  
P J Dyck
Keyword(s):  
Free Cholesterol ◽  
Normal Subjects ◽  
Cholesterol Esterification ◽  
Tween 20 ◽  
Fractional Rate ◽  
Significant Difference ◽  
Reproducible Method ◽  
Esterification Rate ◽  
The Mean

Abstract We report a simple, convenient, and reproducible method, involving the use of radiolabeled cholesterol dispersed in Tween 20 as a tracer and endogenous lipoproteins as a substrate, for measuring the rate of serum cholesterol esterification in vitro. The reaction of lecithin acyltransferase (EC 2.3.1.43) was enhanced by the presence of Tween 20, which probably accelerates the exchange between radiolabeled cholesterol and endogenous lipoprotein cholesterol. In sera from 65 normal subjects, the in vitro cholesterol esterification rate was significantly correlated (r=0.47,P=0.001) with age. The mean rate of esterification of 31 subjects 30 years old or younger was significantly lower than that of 34 subjects 31 to 64 years of age. We found no significant difference in the rate of esterification between men and women. The rate of cholesterol esterification (nmol/ml per h) was significantly correlated with the concentration of endogenous free cholesterol in serum, but the fractional rate (the percentage of radiolabeled cholesterol esterified per hour) was inversely proportional to the endogenous free cholesterol. The fatty acid composition of the cholesteryl esters formed by the acyltransferase reaction may provide an index in recognizing some specific disorder.

13C NMR investigation of cholesterol esterification rate in human whole blood

1995 ◽  
Vol 237 (1-2) ◽  
pp. 25-30 ◽  
Author(s):  
M.Hossein Nouri-Sorkhabi ◽  
David R. Sullivan ◽  
Philip W. Kuchel
Keyword(s):  
13C Nmr ◽  
Whole Blood ◽  
Cholesterol Esterification ◽  
Human Whole Blood ◽  
Esterification Rate

scholarly journals Association of Metabolic and Genetic Factors With Cholesterol Esterification Rate in HDL Plasma and Atherogenic Index of Plasma in a 40 Years Old Slovak Population

2011 ◽  
pp. 785-795 ◽  
Author(s):  
K. RAŠLOVÁ ◽  
M. DOBIÁŠOVÁ ◽  
J. A. HUBÁČEK ◽  
D. BENCOVÁ ◽  
D. SIVÁKOVÁ ◽  
...  
Keyword(s):  
Atherogenic Index ◽  
Cholesterol Esterification ◽  
Atherogenic Index Of Plasma ◽  
Slovak Population ◽  
Novel Biomarkers ◽  
Esterification Rate ◽  
Lipoprotein Particle Size ◽  
Model Variability ◽  
Univariate Analyses ◽  
Conventional Risk Factors

We assessed association between novel biomarkers of cardiovascular disease and conventional factors in 40 years old subjects (208 men and 266 women) from the general population of Slovakia. FERHDL (cholesterol esterification rate in HDL plasma), AIP – Atherogenic Index of Plasma [Log(TG/HDL-C)] as markers of lipoprotein particle size, and CILP2, FTO and MLXIPL polymorphisms, were examined in relation to biomarkers and conventional risk factors. Univariate analyses confirmed correlation between AIP, FERHDL and the most of measured parameters. Relations between AIP and CILP2, FTO and MLXIPL were not significant. However, CILP2 was significantly related to FERHDL in both genders. In multivariate analysis BMI was the strongest correlate of AIP levels. In multivariate model variability of FERHDL was best explained by AIP (R2=0.55) in both genders with still significant effect of CILP2 SNP in men. In a model where AIP was omitted, TG levels explained 43 % of the FERHDL variability in men, while in women HDL-C was the major determinant (42 %). In conclusions, FERHDL and AIP related to the known markers of cardiovascular risk provide means to express their subtle interactions by one number. Our novel finding of association between CILP2 polymorphism and FERHDL supports its role in lipid metabolism.

Sign in / Sign up

Export Citation Format

Share Document