Isolation of the nonprotein nitrogen fraction from human milk by gel-filtration chromatography and its separation by fast protein liquid chromatography

1989 ◽  
Vol 50 (1) ◽  
pp. 53-57 ◽  
Author(s):  
S M Donovan ◽  
B Lönnerdal
Keyword(s):  
Liquid Chromatography ◽  
Human Milk ◽  
Gel Filtration ◽  
Fast Protein Liquid Chromatography ◽  
Nonprotein Nitrogen ◽  
Gel Filtration Chromatography

scholarly journals Lipoproteins Separation on Superose 6 Gel-Filtration Column Using Fast Protein Liquid Chromatography System

1987 ◽  
Vol 15 (5) ◽  
pp. 1179-1183
Author(s):  
Sadao TAKAHASHI ◽  
Toshitaka TAMAI ◽  
Hirotada TAKAI ◽  
Shinta HAYASHI ◽  
Hajime MAEDA ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Gel Filtration ◽  
Fast Protein Liquid Chromatography ◽  
Liquid Chromatography System

Purification of glysojanin, an antifungal protein, from the black soybean Glycine soja

2003 ◽  
Vol 81 (6) ◽  
pp. 387-394 ◽  
Author(s):  
Patrick H.K Ngai ◽  
T B Ng
Keyword(s):  
Liquid Chromatography ◽  
Ion Exchange ◽  
Glycine Soja ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Fast Protein Liquid Chromatography ◽  
Antifungal Protein ◽  
Black Soybean

A monomeric protein, with a molecular mass of 25 kDa and an N-terminal sequence resembling a segment of chitin synthase, was isolated from the seeds of the black soybean Glycine soja. The protein, designated glysojanin, demonstrated potent antifungal activity against the fungi Fusarium oxysporum and Mycosphaerella arachidicola. It inhibited HIV-1 reverse transcriptase with an IC50 of 47 µmol/L, [methyl-3H]thymidine incorporation by mouse spleen cells with an IC50 of 175 µmol/L, and translation in the rabbit reticulocyte lysate with an IC50 of 20 µmol/L. Glysojanin was purified using a procedure that involved ion-exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion-exchange chromatography by fast protein liquid chromatography on Mono S, and gel filtration by fast protein liquid chromatography on Superdex 75.Key words: antifungal protein, seeds, soybean, purification.

Effect of β-glucosidase inhibitors on synthesis of cellulase and β-glucosidase in Sporotrichum (Chrysosporium) thermophile

1991 ◽  
Vol 37 (6) ◽  
pp. 459-464 ◽  
Author(s):  
François L. Y. Carrel ◽  
Giorgio Canevascini
Keyword(s):  
Liquid Chromatography ◽  
Key Words ◽  
Gel Filtration ◽  
Fast Protein Liquid Chromatography ◽  
Cellobiose Dehydrogenase ◽  
The Other ◽  
Cellular Respiration ◽  
Subsequent Growth ◽  
Glucosidase Inhibitors ◽  
Delayed Growth

The addition of nojirimycin or gluconolactam, substances known as β-glucosidase inhibitors, to cultures of Sporotrichum (Chrysosporium) thermophile growing on cellobiose, caused a twofold or even higher increase (inhibited by cycloheximide) of the intracellular activity of this enzyme, but did not stimulate the synthesis of endocellulase or of cellobiose dehydrogenase. At concentrations from 0.1 to 1.0 mM, nojirimycin stimulated β-glucosidase synthesis to an extent apparently independent of concentration but did not affect cellular respiration on cellobiose. At 5 mM, however, cellobiose assimilation and subsequent growth were significantly but only temporarily impaired and β-glucosidase synthesis, which was still stimulated, was correspondingly delayed. Growth on glucose, on the other hand, was completely unaffected by nojirimycin. Upon fast protein liquid chromatography (FPLC) fractionation by gel filtration of mycelial extracts from cultures grown in the presence and in the absence of nojirimycin (0.1 mM), the β-glucosidase was recovered (67–70%) in two distinct fractions, A and B. The observed increase in β-glucosidase activity for cultures with nojirimycin was found to parallel the increase of fraction B. Key words: β-glucosidase inhibition, nojirimycin, cellulase biosynthesis.

scholarly journals Fractionation of charge-modified low density lipoproteins by fast protein liquid chromatography.

1991 ◽  
Vol 32 (8) ◽  
pp. 1359-1369
Author(s):  
B Vedie ◽  
I Myara ◽  
MA Pech ◽  
JC Maziere ◽  
C Maziere ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Low Density Lipoproteins ◽  
Fast Protein Liquid Chromatography ◽  
Low Density ◽  
Modified Low Density Lipoproteins
Sign in / Sign up

Export Citation Format

Share Document