Utilisation of Deep Learning-Enabled Image Analysis for Detection and Enumeration of Plasmodial Forms in Red Cells

2021 ◽  
Vol 156 (Supplement_1) ◽  
pp. S97-S98
Author(s):  
K Hu ◽  
W Welch ◽  
M Pilichowska ◽  
D Bacsa ◽  
J Vandenhirtz
Keyword(s):  
Image Analysis ◽  
Deep Learning ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Red Cells ◽  
Detection Sensitivity ◽  
Red Cell ◽  
Data Set ◽  
Promising Alternative

Abstract Introduction/Objective Objective: This work investigates utilisation of deep learning enabled peripheral blood smear image analysis for automated detection and enumeration of red cell parasites. Methods/Case Report Methods: Peripheral blood smear red cell images from 30 individuals identified as positive and/or negative for plasmodilum falciparum forms were used. Blood submitted to hematology laboratory for complete blood count was evaluated on Sysmex XN 3100 analyzer with routine peripheral blood slides performed on instrument associated SP50 stainer. Images of red cells obtained with Xfinity DX40 microscope mounted camera were subjected to classification using deep learning software (Cognex, ViDi Suite 4.1). The training of the classification tool was performed on 200 peripheral blood smear images divided in two dataset classes: normal/negative and abnormal/positive for plasmodia, 50% of training images represented positive data set. Performance of the developed model was tested on 300 images including 66% positive for plasmodia obtained from 20 patients. Enumeration of parasitic forms was performed for each case. Model performance was compared to expert hematopathology reviewer which was used as gold standard. Results (if a Case Study enter NA) Results: Overall, Cognex ViDi Suite 4.1 demonstrates the effectiveness in discriminating between images positive and negative for red cell plasmodial forms as well as enables parasite quantification. Following performance specifications were determined for parasite detection: sensitivity (0.969230769), specificity (0.99383217). High correlation coefficients (0.9961) between automatically detected parasites and ground truth, on both image level and patient level, demonstrate the practicality of our method. Conclusion Deep learning enabled image analysis of peripheral blood smears is a promising alternative to manual identification and enumeration of red cell plasmodial forms with performance comparable to expert hematopathology reviewer.

scholarly journals A family with red cell pyrimidine 5'-nucleotidase deficiency

Blood ◽  
1976 ◽  
Vol 47 (6) ◽  
pp. 919-922
Author(s):  
I Ben-Bassat ◽  
F Brok-Simoni ◽  
G Kende ◽  
F Holtzmann ◽  
B Ramot
Keyword(s):  
Hemolytic Anemia ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Early Infancy ◽  
Red Cell ◽  
Chronic Hemolytic Anemia ◽  
Congenital Hemolytic Anemia

Congenital hemolytic anemia associated with pyrimidine 5′-nucleotidase deficiency is reported in two siblings. Both have had moderate chronic hemolytic anemia, splenomegaly, and jaundice since early infancy. The peripheral blood smear is characterized by striking red cell basophilic stippling. As this feature has been found in all previously reported cases, it should be the clue to the diagnosis.

scholarly journals Screening for Thalassemia in Healthy Population at a Tertiary Care Hospital in Eastern Nepal

2020 ◽  
Vol 9 (1) ◽  
pp. 41-45
Author(s):  
Manish Kumar Das ◽  
Niraj Nepal ◽  
Prabesh Chaudhary
Keyword(s):  
Peripheral Blood ◽  
Blood Smear ◽  
Tertiary Care ◽  
Age Groups ◽  
Peripheral Blood Smear ◽  
Healthy Population ◽  
Care Hospital ◽  
Red Cell ◽  
Thalassemia Minor ◽  
Red Cell Indices

Background: Thalassemia is a type of congenital anemia, where there is deficient synthesis of one or more type of globin subunits of normal hemoglobin. This study was undertaken with aims & objective to study the prevalence of thalassemia by comparing Red Blood Cell indices, Peripheral Blood Smear and Electrophoresis in adult volunteers. Material and Methods: The study comprised of 518 cases attending hematological department, who were enrolled in our study after proper informed consent, of which 462 cases were further studied. All cases were subjected to blood sampling for estimation of Hemoglobin, red cell indices and peripheral blood smear. Those samples where peripheral blood smear and Red cell indices were suggestive of thalassemia were subjected to Bio-Rad High Performance Liquid Chromatography based electrophoresis to observe the presence of any abnormal hemoglobin. Results: The mean age of screening sample was 42.91 ± 16.85 years with minimum age of 18 years and maximum age of 85 years. The highest number of cases was in between 21-30 years age groups (19.5%) followed by 41-50 years (17.7%). In the study group, 299 (64.7%) cases were male and 163 (35.3%) cases were female. The prevalence of anemia was found to be 48.16% in males and 68.71% in females with overall prevalence of 55.41%. On electrophoresis reports, 19 cases were diagnosed with thalassemia. The only thalassemia observed was thalassemia minor. The prevalence of thalassemia was found to be 4.11%. Conclusion: Significantly high prevalence of thalassemia minor is found in healthy population.

scholarly journals Hemoglobin pyrgos alpha2 beta2 83 (EF7) Gly leads to Asp: a new hemoglobin variant in double heterozygosity with hemoglobin S

Blood ◽  
1976 ◽  
Vol 47 (5) ◽  
pp. 827-832 ◽  
Author(s):  
B Tatsis ◽  
K Sofroniadou ◽  
CI Stergiopoulos
Keyword(s):  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
The Other ◽  
Red Cell ◽  
Hemoglobin Variant ◽  
Hemoglobin S ◽  
Variant Hemoglobin ◽  
Double Heterozygosity ◽  
Old Greek

Abstract An electrophoretically fast-moving hemoglobin variant was found to be present together with hemoglobin S, in the hemolysate of the rythrocytes of at 3-yr-old Greek boy. Electrophoresis of the parents' erythrocyte hemolysates revealed that the father was an AS heterozygote, while the mother was a carrier of the variant hemoglobin. A sibling was also found to be a carrier. The amount of the mutant hemoglobin in the peripheral blood of the propositus, his mother, and his brother was 62.2%, 52.5%, and 51.1%, respectively, as determined by column chromatography. The patients peripheral blood smear showed mild anisocytosis, microcytosis, and hypochromia. Similar but less pronounced red cell abnormalities were found in the other two carriers. Structural analysis of the variant hemoglobin revealed substitution of an aspartic acid for the glycine residue at the beta83 (EF7) position. This new hemoglobin was named hemoglobin Pyrgos. All the carriers of hemoglobin Pyrgos are clinically healthy, and there seems to be no interaction between hemoglobin Pyrgos and hemoglobin S as manifested clinically.

scholarly journals Hemoglobin pyrgos alpha2 beta2 83 (EF7) Gly leads to Asp: a new hemoglobin variant in double heterozygosity with hemoglobin S

Blood ◽  
1976 ◽  
Vol 47 (5) ◽  
pp. 827-832
Author(s):  
B Tatsis ◽  
K Sofroniadou ◽  
CI Stergiopoulos
Keyword(s):  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
The Other ◽  
Red Cell ◽  
Hemoglobin Variant ◽  
Hemoglobin S ◽  
Variant Hemoglobin ◽  
Double Heterozygosity ◽  
Old Greek

An electrophoretically fast-moving hemoglobin variant was found to be present together with hemoglobin S, in the hemolysate of the rythrocytes of at 3-yr-old Greek boy. Electrophoresis of the parents' erythrocyte hemolysates revealed that the father was an AS heterozygote, while the mother was a carrier of the variant hemoglobin. A sibling was also found to be a carrier. The amount of the mutant hemoglobin in the peripheral blood of the propositus, his mother, and his brother was 62.2%, 52.5%, and 51.1%, respectively, as determined by column chromatography. The patients peripheral blood smear showed mild anisocytosis, microcytosis, and hypochromia. Similar but less pronounced red cell abnormalities were found in the other two carriers. Structural analysis of the variant hemoglobin revealed substitution of an aspartic acid for the glycine residue at the beta83 (EF7) position. This new hemoglobin was named hemoglobin Pyrgos. All the carriers of hemoglobin Pyrgos are clinically healthy, and there seems to be no interaction between hemoglobin Pyrgos and hemoglobin S as manifested clinically.

A Case of Congenital Red Cell Pyruvate Kinase Deficiency Associated with Hereditary Spherocytosis

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 5272-5272
Author(s):  
Cristina Vercellati ◽  
Anna Paola Maria Luisa Marcello ◽  
Elisa Fermo ◽  
Paola Bianchi ◽  
Carla Boschetti ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Pyruvate Kinase ◽  
Hemolytic Anemia ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Hereditary Spherocytosis ◽  
Osmotic Fragility ◽  
Peripheral Blood Smear ◽  
Red Cell ◽  
Red Cell Membrane

Abstract Abstract 5272 Pyruvate kinase (PK) deficiency, transmitted as an autosomal recessive trait, is the most common erythroenzymopathy of glycolytic pathway (prevalence of 1:20,000) associated with chronic non spherocytic hemolytic anemia from mild to severe. More than 180 mutations in the PK-LR gene have been so far reported, and genotype-phenotype correlation has been established for some of them. Hereditary Spherocytosis (HS) is the most common congenital hemolytic anemia in Caucasians, with an estimated prevalence ranging from 1:2000 to 1:5000. The main clinical features are hemolytic anemia from compensated to severe, variable jaundice, splenomegaly and cholelythiasis. The molecular defect is highly heterogeneous, caused by proteins involved in the attachment of cytoskeleton to the membrane integral domain (spectrin, ankyrin, band 3 and protein 4.2). We describe a case of PK deficiency associated with HS. The propositus was a 13 years-old Italian male with neonatal jaundice and need of blood transfusion (Hb 5.8 g/dL) during an infectious episode. At the time of the study Hb was 13.9 g/dL, MCV 81.8 fL, reticulocytes 207×109/L, unconjugated bilirubin 2.16 mg/dL, LDH 605 U/L, haptoglobin <20 mg/dL. The peripheral blood smear examination showed the presence of spherocytes (16%) and some ovalocytes (2%). The study of the most important red cell enzymes revealed reduced PK activity (59% of normal). Direct sequencing of PK-LR gene showed compound heterozygosity for the 994A mutation (Gly332Ser) and the −148T variant localized the erythroid specific promoter region. The presence of spherocytes in peripheral blood smear prompted us to investigate for the coexistence of HS. Erythrocyte osmotic fragility was decreased and SDS–PAGE analysis of red cell membrane proteins revealed a 30% spectrin reduction. Family study demonstrated a heterozygous condition for the 994A mutation in the father, who also displayed comparable enzyme deficiency, whereas promoter variant −148T was detected in the mother and in the brother. No red cell membrane abnormalities were present in the family members, although positive EMA binding test and increased osmotic fragility were found in the father and brother. The co-existence of HS and PK deficiency is very rare event, only few cases are described to date. Clinical, family and molecular studies allowed the determination of the interrelationship between the two RBC abnormalities in the patient and his relatives. The reduced PK activity in the propositus and his father is justified by heterozygous 994A mutation. The more severe clinical picture in the propositus could be caused by the coexistence of HS and by the presence of −148T mutation, that although it seems not to have effects on PK-LR mRNA expression, is often detected in PK deficient subjects with heterozygous PK mutations. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Peripheral blood smear image analysis: A comprehensive review

2014 ◽  
Vol 5 (1) ◽  
pp. 9 ◽  
Author(s):  
Christopher Naugler ◽  
EmadA Mohammed ◽  
MostafaM. A. Mohamed ◽  
BehrouzH Far
Keyword(s):  
Image Analysis ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Comprehensive Review ◽  
Blood Smear Image

scholarly journals A family with red cell pyrimidine 5'-nucleotidase deficiency

Blood ◽  
1976 ◽  
Vol 47 (6) ◽  
pp. 919-922 ◽  
Author(s):  
I Ben-Bassat ◽  
F Brok-Simoni ◽  
G Kende ◽  
F Holtzmann ◽  
B Ramot
Keyword(s):  
Hemolytic Anemia ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Early Infancy ◽  
Red Cell ◽  
Chronic Hemolytic Anemia ◽  
Congenital Hemolytic Anemia

Abstract Congenital hemolytic anemia associated with pyrimidine 5′-nucleotidase deficiency is reported in two siblings. Both have had moderate chronic hemolytic anemia, splenomegaly, and jaundice since early infancy. The peripheral blood smear is characterized by striking red cell basophilic stippling. As this feature has been found in all previously reported cases, it should be the clue to the diagnosis.

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