scholarly journals Targeted metabolic reconstruction: a novel approach for the characterization of plant-pathogen interactions

2010 ◽  
Vol 12 (2) ◽  
pp. 151-162 ◽  
Author(s):  
A. Pinzon ◽  
L. M. Rodriguez-R ◽  
A. Gonzalez ◽  
A. Bernal ◽  
S. Restrepo
Keyword(s):  
Plant Pathogen ◽  
Metabolic Reconstruction ◽  
Novel Approach ◽  
Plant Pathogen Interactions

scholarly journals Hyperspectral phenotyping on the microscopic scale: towards automated characterization of plant-pathogen interactions

Plant Methods ◽  
2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Matheus Kuska ◽  
Mirwaes Wahabzada ◽  
Marlene Leucker ◽  
Heinz-Wilhelm Dehne ◽  
Kristian Kersting ◽  
...  
Keyword(s):  
Plant Pathogen ◽  
Microscopic Scale ◽  
Plant Pathogen Interactions

Dual metabolomics: A novel approach to understanding plant–pathogen interactions

2010 ◽  
Vol 71 (5-6) ◽  
pp. 590-597 ◽  
Author(s):  
J. William Allwood ◽  
Andrew Clarke ◽  
Royston Goodacre ◽  
Luis A.J. Mur
Keyword(s):  
Plant Pathogen ◽  
Novel Approach ◽  
Plant Pathogen Interactions

scholarly journals Aspergillus Infection Inhibits the Expression of Peanut 13S-HPODE-Forming Seed Lipoxygenases

2005 ◽  
Vol 18 (10) ◽  
pp. 1081-1089 ◽  
Author(s):  
Dimitrios I. Tsitsigiannis ◽  
Susan Kunze ◽  
David K. Willis ◽  
Ivo Feussner ◽  
Nancy P. Keller
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Aspergillus Flavus ◽  
Plant Pathogen ◽  
Peanut Seed ◽  
Aspergillus Infection ◽  
Resistance Factors ◽  
Plant Pathogen Interactions ◽  
Susceptibility And Resistance

Oxylipins recently have been implicated as signaling molecules for cross-kingdom communication in plant-pathogen interactions. Linoleic acid and its two plant lipoxygenase (LOX) oxylipin products 9- and 13-hydroperoxy fatty acids (9S- and 13S-HPODE) have been shown to have a significant effect on differentiation processes in the mycotoxigenic seed pathogens Aspergillus spp. Whereas both fatty acids promote sporulation, 9S-HPODE stimulates and 13S-HPODE inhibits mycotoxin production. Additionally, Aspergillus flavus infection of seed promotes linoleate 9-LOX expression and 9S-HPODE accumulation. Here, we describe the characterization of two peanut seed lipoxygenase alleles (PnLOX2 and PnLOX3) highly expressed in mature seed. PnLOX2 and PnLOX3 both are 13S-HPODE producers (linoleate 13-LOX) and, in contrast to previously characterized 9-LOX or mixed function LOX genes, are repressed between 5-fold and 250-fold over the course of A. flavus infection. The results of these studies suggest that 9SHPODE and 13S-HPODE molecules act as putative susceptibility and resistance factors respectively, in Aspergillus seed-aflatoxin interactions.

scholarly journals Characterization of a Secretory YML079-like Cupin Protein That Contributes to Sclerotinia sclerotiorum Pathogenicity

2021 ◽  
Vol 9 (12) ◽  
pp. 2519
Author(s):  
Hongxia Fan ◽  
Wenwen Yang ◽  
Jiayue Nie ◽  
Chen Lin ◽  
Jian Wu ◽  
...  
Keyword(s):  
Oilseed Rape ◽  
Sclerotinia Sclerotiorum ◽  
Plant Pathogen ◽  
Effector Protein ◽  
Double Stranded Rna ◽  
Plant Pathogen Interactions

Sclerotinia sclerotiorum causes devastating diseases in many agriculturally important crops, including oilseed rape and sunflower. However, the mechanisms of Sclerotinia sclerotiorum pathogenesis remain poorly understood. In this study, we characterized a YML079-like cupin protein (SsYCP1) from Sclerotinia sclerotiorum. We showed that SsYCP1 is strongly expressed and secreted during Sclerotinia sclerotiorum infection. Sclerotinia sclerotiorum infection was promoted by SsYCP1 overexpression and inhibited by silencing this gene with synthetic double-stranded RNA. These results collectively indicate SsYCP1 as a putative effector protein that contributes to Sclerotinia sclerotiorum pathogenicity. These findings extend our understanding of effector-mediated Sclerotinia sclerotiorum pathogenesis and suggest a novel role for YML079-like cupin proteins in plant–pathogen interactions.

scholarly journals Plant SWEETs: from sugar transport to plant–pathogen interaction and more unexpected physiological roles

2021 ◽  
Author(s):  
Richard Breia ◽  
Artur Conde ◽  
Hélder Badim ◽  
Ana Margarida Fortes ◽  
Hernâni Gerós ◽  
...  
Keyword(s):  
Plant Pathogen ◽  
Sugar Transport ◽  
High Capacity ◽  
Phloem Loading ◽  
Transcription Activator ◽  
Pythium Irregulare ◽  
Clear Cut ◽  
Opposite Behavior ◽  
Plant Pathogen Interaction ◽  
Plant Pathogen Interactions

Abstract Sugars Will Eventually be Exported Transporters (SWEETs) have important roles in numerous physiological mechanisms where sugar efflux is critical, including phloem loading, nectar secretion, seed nutrient filling, among other less expected functions. They mediate low affinity and high capacity transport, and in angiosperms this family is composed by 20 paralogs on average. As SWEETs facilitate the efflux of sugars, they are highly susceptible to hijacking by pathogens, making them central players in plant–pathogen interaction. For instance, several species from the Xanthomonas genus are able to upregulate the transcription of SWEET transporters in rice (Oryza sativa), upon the secretion of transcription-activator-like effectors. Other pathogens, such as Botrytis cinerea or Erysiphe necator, are also capable of increasing SWEET expression. However, the opposite behavior has been observed in some cases, as overexpression of the tonoplast AtSWEET2 during Pythium irregulare infection restricted sugar availability to the pathogen, rendering plants more resistant. Therefore, a clear-cut role for SWEET transporters during plant–pathogen interactions has so far been difficult to define, as the metabolic signatures and their regulatory nodes, which decide the susceptibility or resistance responses, remain poorly understood. This fuels the still ongoing scientific question: what roles can SWEETs play during plant–pathogen interaction? Likewise, the roles of SWEET transporters in response to abiotic stresses are little understood. Here, in addition to their relevance in biotic stress, we also provide a small glimpse of SWEETs importance during plant abiotic stress, and briefly debate their importance in the particular case of grapevine (Vitis vinifera) due to its socioeconomic impact.

scholarly journals Application of Innovative TGA/Chemometric Approach for Forensic Purposes: The Estimation of the Time since Death in Contaminated Specimens

Diagnostics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 121
Author(s):  
Roberta Risoluti ◽  
Giuseppina Gullifa ◽  
Vittorio Fineschi ◽  
Paola Frati ◽  
Stefano Materazzi
Keyword(s):  
Multidisciplinary Approach ◽  
Vitreous Humor ◽  
Classification Model ◽  
Forensic Sciences ◽  
Time Since Death ◽  
Time Of Death ◽  
Novel Approach ◽  
Chemometric Approach ◽  
Postmortem Examinations

Chronothanatology has always been a challenge in forensic sciences. Therefore, the importance of a multidisciplinary approach for the characterization of matrices (organs, tissues, or fluids) that respond linearly to the postmortem interval (PMI) is emerging increasingly. The vitreous humor is particularly suitable for studies aimed at assessing time-related modifications because it is topographically isolated and well-protected. In this work, a novel approach based on thermogravimetry and chemometrics was used to estimate the time since death in the vitreous humor and to collect a databank of samples derived from postmortem examinations after medico–legal evaluation. In this study, contaminated and uncontaminated specimens with tissue fragments were included in order to develop a classification model to predict time of death based on partial least squares discriminant analysis (PLS-DA) that was as robust as possible. Results demonstrate the possibility to correctly predict the PMI even in contaminated samples, with an accuracy not lower than 70%. In addition, the correlation coefficient of the measured versus predicted outcomes was found to be 0.9978, confirming the ability of the model to extend its feasibility even to such situations involving contaminated vitreous humor.

scholarly journals Visualising the ionome in resistant and susceptible plant‐pathogen interactions

2021 ◽  
Author(s):  
S.M. Brouwer ◽  
P. Lindqvist‐Reis ◽  
D. Pergament Persson ◽  
S. Marttila ◽  
L.J. Grenville‐Briggs ◽  
...  
Keyword(s):  
Plant Pathogen ◽  
Susceptible Plant ◽  
Plant Pathogen Interactions
Sign in / Sign up

Export Citation Format

Share Document