scholarly journals Characterization of a Secretory YML079-like Cupin Protein That Contributes to Sclerotinia sclerotiorum Pathogenicity

2021 ◽  
Vol 9 (12) ◽  
pp. 2519
Author(s):  
Hongxia Fan ◽  
Wenwen Yang ◽  
Jiayue Nie ◽  
Chen Lin ◽  
Jian Wu ◽  
...  
Keyword(s):  
Oilseed Rape ◽  
Sclerotinia Sclerotiorum ◽  
Plant Pathogen ◽  
Effector Protein ◽  
Double Stranded Rna ◽  
Plant Pathogen Interactions

Sclerotinia sclerotiorum causes devastating diseases in many agriculturally important crops, including oilseed rape and sunflower. However, the mechanisms of Sclerotinia sclerotiorum pathogenesis remain poorly understood. In this study, we characterized a YML079-like cupin protein (SsYCP1) from Sclerotinia sclerotiorum. We showed that SsYCP1 is strongly expressed and secreted during Sclerotinia sclerotiorum infection. Sclerotinia sclerotiorum infection was promoted by SsYCP1 overexpression and inhibited by silencing this gene with synthetic double-stranded RNA. These results collectively indicate SsYCP1 as a putative effector protein that contributes to Sclerotinia sclerotiorum pathogenicity. These findings extend our understanding of effector-mediated Sclerotinia sclerotiorum pathogenesis and suggest a novel role for YML079-like cupin proteins in plant–pathogen interactions.

scholarly journals Overexpression of Brassica napus MPK4 Enhances Resistance to Sclerotinia sclerotiorum in Oilseed Rape

2009 ◽  
Vol 22 (3) ◽  
pp. 235-244 ◽  
Author(s):  
Zheng Wang ◽  
Han Mao ◽  
Caihua Dong ◽  
Ruiqin Ji ◽  
Li Cai ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Transgenic Plants ◽  
Oilseed Rape ◽  
Sclerotinia Sclerotiorum ◽  
Mitogen Activated Protein Kinase ◽  
Economic Losses ◽  
Loss Of Function ◽  
Mitogen Activated Protein ◽  
Transgenic Oilseed Rape ◽  
Plant Pathogen Interactions

Sclerotinia sclerotiorum causes a highly destructive disease in oilseed rape (Brassica napus) resulting in significant economic losses. Studies on the Arabidopsis thaliana MPK4 loss-of-function mutant have implicated that AtMPK4 is involved in plant defense regulation, and its effect on disease resistance varies in different plant–pathogen interactions. In this study, we isolated a B. napus mitogen-activated protein kinase, BnMPK4, and found that BnMPK4 along with PDF1.2 are inducible in resistant line Zhongshuang9 but both are consistently suppressed in susceptible line 84039 after inoculation with S. sclerotiorum. Transgenic oilseed rape overexpressing BnMPK4 markedly enhances resistance to S. sclerotiorum and Botrytis cinerea. Further experiments showed that transgenic plants inhibited growth of S. sclerotiorum and constitutively activated PDF1.2 but decreased H2O2 production and constitutively suppressed PR-1 expression. Treatment of roots of the transgenic plants with H2O2 solution resulted in enhanced susceptibility to the two pathogens. Our results support the idea that MPK4 positively regulates jasmonic acid-mediated defense response, which might play an important role in resistance to S. sclerotiorum in oilseed rape.

scholarly journals Benzyl and Phenyl Glucosinolates Are Metabolized by the Specific Plant Pathogen Alternaria Brassicicola but Not by the Generalist Fungal Pathogens Rhizoctonia Solani or Sclerotinia Sclerotiorum

2021 ◽  
Author(s):  
M. Soledade C. Pedras ◽  
Chintamani Thapa ◽  
Sajjad Hossain
Keyword(s):  
Carboxylic Acid ◽  
Rhizoctonia Solani ◽  
Sclerotinia Sclerotiorum ◽  
Plant Pathogen ◽  
Fungal Pathogens ◽  
Spectroscopic Characterization ◽  
Fungal Species ◽  
Alternaria Brassicicola ◽  
Metabolic Products

The metabolism of benzyl and phenyl glucosinolates by three phytopathogenic fungal species is investigated and established that <i>A. brassicicola </i>metabolized intact benzyl and phenyl glucosinolates and the corresponding desulfo derivatives. Syntheses and spectroscopic characterization of benzyl and phenyl desulfo-glucosinolates are reported. Phenylacetonitrile and benzylisothiocyanate are the first metabolic products of benzyl glucosinolate; benzyl isothiocyanate is further metabolized to 3-benzyl-2-thioxo-2,3-dihydrothiazolidine-4-carboxylic acid.

scholarly journals Targeted metabolic reconstruction: a novel approach for the characterization of plant-pathogen interactions

2010 ◽  
Vol 12 (2) ◽  
pp. 151-162 ◽  
Author(s):  
A. Pinzon ◽  
L. M. Rodriguez-R ◽  
A. Gonzalez ◽  
A. Bernal ◽  
S. Restrepo
Keyword(s):  
Plant Pathogen ◽  
Metabolic Reconstruction ◽  
Novel Approach ◽  
Plant Pathogen Interactions

scholarly journals Hyperspectral phenotyping on the microscopic scale: towards automated characterization of plant-pathogen interactions

Plant Methods ◽  
2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Matheus Kuska ◽  
Mirwaes Wahabzada ◽  
Marlene Leucker ◽  
Heinz-Wilhelm Dehne ◽  
Kristian Kersting ◽  
...  
Keyword(s):  
Plant Pathogen ◽  
Microscopic Scale ◽  
Plant Pathogen Interactions

scholarly journals Benzyl and Phenyl Glucosinolates Are Metabolized by the Specific Plant Pathogen Alternaria Brassicicola but Not by the Generalist Fungal Pathogens Rhizoctonia Solani or Sclerotinia Sclerotiorum

2021 ◽  
Author(s):  
M. Soledade C. Pedras ◽  
Chintamani Thapa ◽  
Sajjad Hossain
Keyword(s):  
Carboxylic Acid ◽  
Rhizoctonia Solani ◽  
Sclerotinia Sclerotiorum ◽  
Plant Pathogen ◽  
Fungal Pathogens ◽  
Spectroscopic Characterization ◽  
Fungal Species ◽  
Alternaria Brassicicola ◽  
Metabolic Products

The metabolism of benzyl and phenyl glucosinolates by three phytopathogenic fungal species is investigated and established that <i>A. brassicicola </i>metabolized intact benzyl and phenyl glucosinolates and the corresponding desulfo derivatives. Syntheses and spectroscopic characterization of benzyl and phenyl desulfo-glucosinolates are reported. Phenylacetonitrile and benzylisothiocyanate are the first metabolic products of benzyl glucosinolate; benzyl isothiocyanate is further metabolized to 3-benzyl-2-thioxo-2,3-dihydrothiazolidine-4-carboxylic acid.

scholarly journals Aspergillus Infection Inhibits the Expression of Peanut 13S-HPODE-Forming Seed Lipoxygenases

2005 ◽  
Vol 18 (10) ◽  
pp. 1081-1089 ◽  
Author(s):  
Dimitrios I. Tsitsigiannis ◽  
Susan Kunze ◽  
David K. Willis ◽  
Ivo Feussner ◽  
Nancy P. Keller
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Aspergillus Flavus ◽  
Plant Pathogen ◽  
Peanut Seed ◽  
Aspergillus Infection ◽  
Resistance Factors ◽  
Plant Pathogen Interactions ◽  
Susceptibility And Resistance

Oxylipins recently have been implicated as signaling molecules for cross-kingdom communication in plant-pathogen interactions. Linoleic acid and its two plant lipoxygenase (LOX) oxylipin products 9- and 13-hydroperoxy fatty acids (9S- and 13S-HPODE) have been shown to have a significant effect on differentiation processes in the mycotoxigenic seed pathogens Aspergillus spp. Whereas both fatty acids promote sporulation, 9S-HPODE stimulates and 13S-HPODE inhibits mycotoxin production. Additionally, Aspergillus flavus infection of seed promotes linoleate 9-LOX expression and 9S-HPODE accumulation. Here, we describe the characterization of two peanut seed lipoxygenase alleles (PnLOX2 and PnLOX3) highly expressed in mature seed. PnLOX2 and PnLOX3 both are 13S-HPODE producers (linoleate 13-LOX) and, in contrast to previously characterized 9-LOX or mixed function LOX genes, are repressed between 5-fold and 250-fold over the course of A. flavus infection. The results of these studies suggest that 9SHPODE and 13S-HPODE molecules act as putative susceptibility and resistance factors respectively, in Aspergillus seed-aflatoxin interactions.

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