Cloprostenol, a synthetic analogue of prostaglandin F2α induces functional regression in cultured luteal cells of felids†

2021 ◽  
Author(s):  
Michał M Hryciuk ◽  
Katarina Jewgenow ◽  
Beate C Braun
Keyword(s):  
Expression Analysis ◽  
Hormone Receptors ◽  
Cultured Cells ◽  
Prostaglandin F2α ◽  
Domestic Cat ◽  
Synthetic Analogue ◽  
Short Term Treatment ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Maintenance Stage

Abstract In the present study, we investigated the effect of the synthetic analogue of prostaglandin F2α (PGF2α)—cloprostenol—on cultured steroidogenic luteal cells of selected felid species over a two day culture period. The changes induced by cloprostenol were measured based on progesterone concentration and mRNA expression analysis of selected genes. Cloprostenol significantly reduced concentration of progesterone in cell culture medium of small luteal cells isolated from domestic cat corpora lutea (CL) at the development/maintenance stage (p < 0.05), but did not influence progesterone production in cultured cells from the regression stage. A decrease or complete silencing of progesterone production was also measured in cultured luteal cells of African lion (formation stage) and Javan leopard (development/maintenance stage). Gene expression analysis by real-time PCR revealed that treatment with cloprostenol did not have an influence on expression of selected genes coding for enzymes of steroidogenesis (StAR, HSD3B, CYP11A1) or prostaglandin synthesis (PTGS2, PGES), nor did it effect hormone receptors (AR, ESR1, PGR, PTGER2), an anti-oxidative enzyme (SOD1) or factors of cell apoptosis (FAS, CASP3, TNFRSF1B, BCL2) over the studied period. Significant changes were measured only for expressions of luteinizing hormone (P < 0.05), prolactin (P < 0.05) and PGF2α receptors (P < 0.005) (LHCGR, PRLR and PTGFR). The obtained results confirm that PGF2α/cloprostenol is a luteolytic agent in CL of felids and its impact on progesterone production depends on the developmental stage of the CL. Cloprostenol short term treatment on luteal cells was associated only with functional but not structural changes related to luteal regression.

60 EFFECTS OF CONCANAVALIN A ON THE PROGESTERONE PRODUCTION BY BOVINE STEROIDOGENIC LUTEAL CELLS IN VITRO

2017 ◽  
Vol 29 (1) ◽  
pp. 137
Author(s):  
F. C. Destro ◽  
I. Martin ◽  
F. D. C. Landim-Alvarenga ◽  
R. Sartori Filho ◽  
J. L. Pate ◽  
...  
Keyword(s):  
Concanavalin A ◽  
Culture Media ◽  
Prostaglandin F2α ◽  
Inhibitory Effect ◽  
Humid Atmosphere ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Fetal Bovine ◽  
Culture Differences

The corpus luteum is a temporary organ that is responsible for progesterone (P4) secretion and is essential for the establishment and maintenance of pregnancy in cattle. Concanavalin A (CONA) is a lectin that was originally extracted from the Jack bean (Canavalia ensiformis) and that interacts with several kinds of cells, including immune cells and luteal cells. The aim of the present study was to evaluate the effects of CONA on the P4 production by bovine steroidogenic luteal cells (LC) in vitro. Luteal cells were collected during the mid-luteal stage (at 10–12 days following ovulation) and processed in the laboratory. Luteal cells were grown for 7 days in a humid atmosphere with 5% CO2, with or without 10% fetal bovine serum (FBS), and were subjected to the following treatments: control: no treatment; CONA (10 μg mL−1); LH (100 μg mL−1); CONA+LH; LH (100 μg mL−1) + prostaglandin F2α (PGF2α; 10 ng mL−1); CONA+LH+PGF2α. Samples of the culture media were collected on Day 1 and Day 7 for P4 quantification. The cells were counted on Day 7 of culture. Differences between treatments were considered statistically significant at P < 0.05. The P4 concentration in the culture media was numerically greater on Day 1 (558.0 ng mL−1) than on Day 7 (25.4 ng mL−1). The P4 concentration in the culture media was numerically greater for treatments with 10% FBS than for the FBS-free treatments, and the presence of CONA decreased LC P4-secreting capacity. This effect required more than 24 h of exposure to CONA to be fully manifested. On Day 1 of culture, CONA had no effect on P4 production of LC cultured in serum-free medium (P > 0.05).The suppressive action of CONA was more pronounced for cultures without FBS. By Day 7 of culture, the effects of CONA on P4 production were readily apparent. In the absence of serum, CONA had a highly significant (P < 0.01) inhibitory effect on basal progesterone production, as well as in the presence of LH or LH + PGF. In the presence of FBS, there was a tendency for decreased P4 in response to CONA in the LH- and the LH + PGF-treated cells (P = 0.090 and 0.085, respectively). The number of the cells present on Day 7 was not affected by the treatments tested (P > 0.05). More studies are required to better understand the effect of CONA on the P4 production of bovine LC. Financial support from FAPESP is acknowledged: grant no. 2013/00992–3, grant no. 2013/07439–8, and grant no. 2015/01940–2.

Prostaglandin F2α- and phorbol 12-myristate-13-acetate-stimulated progesterone production by cultured human luteal cells in the mid-luteal phase: prostaglandin F2α increases cytosolic Ca2+ and inositol phosphates

1992 ◽  
Vol 133 (3) ◽  
pp. 451-458 ◽  
Author(s):  
T. Endo ◽  
H. Watanabe ◽  
H. Yamamoto ◽  
S. Tanaka ◽  
M. Hashimoto
Keyword(s):  
Luteal Phase ◽  
Inositol Phosphates ◽  
Prostaglandin F2α ◽  
Free Calcium ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Kinase C ◽  
Prostaglandin F ◽  
Human Corpus Luteum

ABSTRACT While prostaglandin F2α (PGF2α) has been thought to be a natural luteolysin in non-primates, a luteolytic effect in the human corpus luteum is less evident. We therefore investigated the action of PGF2α on monolayer cultures of human luteal cells obtained from mid-luteal phase corpora lutea. PGF2α increased basal and human chorionic gonadotrophin (hCG)-stimulated progesterone production by human cultured luteal cells. A potent tumour-promoting phorbol ester, phorbol 12-myristate-13-acetate (PMA), also stimulated progesterone production by cultured human luteal cells. Although human luteal cells were incubated for 24 h with PMA, hCG was still able to stimulate the production of progesterone by PMA-pretreated cells. However, PMA pretreatment blocked the ability of PGF2α to stimulate progesterone production. It is possible that the luteotrophic effect of PGF2α may be mediated, in part, by the activation of protein kinase C. Addition of PGF2α to suspensions of human luteal cells preincubated with myo-[2-3H]inositol promoted an increase in labelled inositol phosphates. PGF2α also rapidly increased intracellular free Ca2+ in human luteal cells loaded with the fluorescent Ca2+ probe, fura-2. We conclude that PGF2α and PMA stimulate progesterone production and that PGF2α increases the intracellular free calcium and inositol phosphates of human cultured luteal cells in the mid-luteal phase. Journal of Endocrinology (1992) 133, 451–458

scholarly journals Estrogen promotes luteolysis by redistributing prostaglandin F2α receptors within primate luteal cells

Reproduction ◽  
2015 ◽  
Vol 149 (5) ◽  
pp. 453-464 ◽  
Author(s):  
Soon Ok Kim ◽  
Nune Markosyan ◽  
Gerald J Pepe ◽  
Diane M Duffy
Keyword(s):  
Estrogen Receptor ◽  
Granulosa Cells ◽  
Prostaglandin F2α ◽  
Luteal Cell ◽  
Corpora Lutea ◽  
Dependent Manner ◽  
Perinuclear Region ◽  
Luteal Cells ◽  
Progesterone Production

Prostaglandin F2α (PGF2α) has been proposed as a functional luteolysin in primates. However, administration of PGF2α or prostaglandin synthesis inhibitors in vivo both initiate luteolysis. These contradictory findings may reflect changes in PGF2α receptors (PTGFRs) or responsiveness to PGF2α at a critical point during the life span of the corpus luteum. The current study addressed this question using ovarian cells and tissues from female cynomolgus monkeys and luteinizing granulosa cells from healthy women undergoing follicle aspiration. PTGFRs were present in the cytoplasm of monkey granulosa cells, while PTGFRs were localized in the perinuclear region of large, granulosa-derived monkey luteal cells by mid-late luteal phase. A PTGFR agonist decreased progesterone production in luteal cells obtained at mid-late and late luteal phases, but did not decrease progesterone production by granulosa cells or luteal cells from younger corpora lutea. These findings are consistent with a role for perinuclear PTGFRs in functional luteolysis. This concept was explored using human luteinizing granulosa cells maintained in vitro as a model for luteal cell differentiation. In these cells, PTGFRs relocated from the cytoplasm to the perinuclear area in an estrogen- and estrogen receptor-dependent manner. Similar to our findings with monkey luteal cells, human luteinizing granulosa cells with perinuclear PTGFRs responded to a PTGFR agonist with decreased progesterone production. These data support the concept that PTGFR stimulation promotes functional luteolysis only when PTGFRs are located in the perinuclear region. Estrogen receptor-mediated relocation of PTGFRs within luteal cells may be a necessary step in the initiation of luteolysis in primates.

A POSSIBLE INTERRELATIONSHIP BETWEEN GONADOTROPHIN STIMULATION AND PROSTAGLANDIN F2α INHIBITION OF STEROIDOGENESIS BY GRANULOSA-LUTEAL CELLS IN VITRO

1977 ◽  
Vol 73 (1) ◽  
pp. 71-78 ◽  
Author(s):  
K. M. HENDERSON ◽  
K. P. McNATTY
Keyword(s):  
Tissue Culture ◽  
Corpus Luteum ◽  
Prostaglandin F2α ◽  
Luteal Cell ◽  
Luteal Cells ◽  
Lytic Action ◽  
Progesterone Production ◽  
Prostaglandin F

SUMMARY The newly formed corpus luteum of many species is refractory to the lytic action of prostaglandin F2α (PGF2α). This phenomenon was studied utilizing porcine, bovine and human granulosa-luteal cells in tissue culture. The steroidogenic potential of the granulosa-luteal cells was critical in determining whether PGF2α could inhibit progesterone production. Since the steroidogenic potential of the granulosa-luteal cell is related to the amount of LH bound to the cell, the bound LH may protect the granulosa-luteal cells from the lytic action of PGF2α. Finally, a 'see-saw' type of interaction between LH and PGF2α is postulated to account for the resistance of the newly formed corpus luteum to PGF2α

scholarly journals Astaxanthin increases progesterone production in cultured bovine luteal cells

2017 ◽  
Vol 79 (6) ◽  
pp. 1103-1109 ◽  
Author(s):  
Hachiro KAMADA ◽  
Satoshi AKAGI ◽  
Shinya WATANABE
Keyword(s):  
Luteal Cells ◽  
Progesterone Production

Lymphocytes stimulate progesterone production by cultured human granulosa luteal cells

1991 ◽  
Vol 165 (5) ◽  
pp. 1469-1474 ◽  
Author(s):  
Nobuyuki Emi ◽  
Hideharu Kanzaki ◽  
Masumi Yoshida ◽  
Kenji Takakura ◽  
Masatoshi Kariya ◽  
...  
Keyword(s):  
Luteal Cells ◽  
Progesterone Production
Sign in / Sign up

Export Citation Format

Share Document