Exposure of Adult Rats to Phthalate (DBP) or Estrogen (DES) Causes a Shift in the Adult-Type Leydig Cell Proliferation/Differentiation Trajectory.

2012 ◽  
Vol 87 (Suppl_1) ◽  
pp. 78-78
Author(s):  
Richard Ivell ◽  
Kee Heng ◽  
Ravinder Anand-Ivell
Keyword(s):  
Cell Proliferation ◽  
Leydig Cell ◽  
Adult Rats ◽  
Adult Type

scholarly journals Transient Hypothyroidism: Dual Effect on Adult-Type Leydig Cell and Sertoli Cell Development

2017 ◽  
Vol 8 ◽  
Author(s):  
Eddy Rijntjes ◽  
Marcos L. M. Gomes ◽  
Nina Zupanič ◽  
Hans J. M. Swarts ◽  
Jaap Keijer ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Leydig Cell ◽  
Cell Development ◽  
Dual Effect ◽  
Adult Type ◽  
Transient Hypothyroidism

Testicular serotonin is related to mast cells but not to Leydig cells in the rat

1995 ◽  
Vol 146 (1) ◽  
pp. 15-21 ◽  
Author(s):  
R Aguilar ◽  
F Antón ◽  
C Bellido ◽  
E Aguilar ◽  
F Gaytan
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Leydig Cell ◽  
Leydig Cells ◽  
Interstitial Fluid ◽  
Ventral Prostate ◽  
Adult Rats ◽  
Cell Numbers ◽  
Oestradiol Benzoate ◽  
Testicular Capsule

Abstract Testicular serotonin (5HT) concentrations were determined by HPLC in the testes of rats treated neonatally with oestradiol benzoate (EB) and in adult rats treated with the Leydig cell cytotoxic ethylene dimethane sulphonate (EDS). 5HT concentrations were related to mast cell numbers. EB-treated rats showed an accumulation of mast cells in the testes at 35 and 70 days of age and increased 5HT concentrations in both the interstitial fluid and the testicular capsule, whereas no increases in 5HT concentrations or in the number of mast cells were found for the ventral prostate of these animals. On the contrary, 5HT concentrations were not related to the number of Leydig cells. In EB-treated rats, in which Leydig cells were nearly absent at 35 days of age, 5HT concentrations were significantly increased. Furthermore, EDS-treated rats did not show significant changes in 5HT concentrations, in spite of the elimination of Leydig cells. These data suggest that mast cells are a major source of serotonin in the rat testis. Journal of Endocrinology (1995) 146, 15–21

scholarly journals Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach

2017 ◽  
Vol 67 (1) ◽  
pp. 1-10
Author(s):  
Gordana Joksić ◽  
Mileva Mićić ◽  
Jelena Filipović ◽  
Dunja Drakulić ◽  
Miloš Stanojlović ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Immunohistochemical Analysis ◽  
Optimal Dose ◽  
Cell Labeling ◽  
Assay Method ◽  
Proliferating Cells ◽  
Adult Rats ◽  
In Vivo Labeling

AbstractThe study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2′-deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2′-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2′-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2′-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2′-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2′-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2′-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.

Cell Proliferation in the Brains of Adult Rats Exposed to Traumatic Brain Injury

2013 ◽  
pp. 27-38
Author(s):  
Sandra A. Acosta ◽  
Naoki Tajiri ◽  
Paula C. Bickford ◽  
Cesar V. Borlongan
Keyword(s):  
Traumatic Brain Injury ◽  
Cell Proliferation ◽  
Brain Injury ◽  
Adult Rats

scholarly journals Screening of the Active Component Promoting Leydig Cell Proliferation from Lepidium meyenii Using HPLC-ESI-MS/MS Coupled with Multivariate Statistical Analysis

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2101 ◽  
Author(s):  
Xiao-chen Gao ◽  
Jing-wei Lv ◽  
Chun-nan Li ◽  
Nan-xi Zhang ◽  
Lin-lin Tian ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Leydig Cell ◽  
Active Sites ◽  
Docking Simulation ◽  
Cyclophilin D ◽  
Quick Method ◽  
Specific Chemical ◽  
Hplc Fingerprint ◽  
Lepidium Meyenii ◽  
Testosterone Secretion

Lepidium meyenii is now widely consumed as a functional food and medicinal product, which is known as an enhancer of reproductive health. However, the specific chemical composition and mechanism of action for improving sexual function are unclear. The present study aims at screening and determining the potential compounds, which promote mouse leydig cells (TM3) proliferation. The partial least squares analysis (PLS) was employed to reveal the correlation between common peaks of high performance liquid chromatography (HPLC) fingerprint of L. meyenii and the proliferation activity of TM3. The results suggested that three compounds had good activities on the proliferation of TM3 and promoting testosterone secretion, there were N-benzyl-hexadecanamide, N-benzyl-(9z,12z)-octadecadienamide and N-benzyl-(9z,12z,15z)-octadecatrienamide which might be the potential bioactive markers related to the enhancing sexual ability functions of L. meyenii. The first step in testosterone synthesis is the transport of cholesterol into the mitochondria, and the homeostasis of mitochondrial function is related to cyclophilin D (CypD). In order to expound how bioactive ingredients lead to promoting testosterone secretion, a molecular docking simulation was used for further illustration in the active sites and binding degree of the ligands on CypD. The results indicated there was a positive correlation between the binding energy absolute value and testosterone secretion activity. In addition, in this study it also provided the reference for a simple, quick method to screen the promoting leydig cell proliferation active components in traditional Chinese medicine (TCM).

Insulin-like growth factor-I mRNA expression in the interstitial cells of the rat testis

1993 ◽  
Vol 11 (3) ◽  
pp. 319-324 ◽  
Author(s):  
A Moore ◽  
C-L C Chen ◽  
J R E Davis ◽  
I D Morris
Keyword(s):  
Leydig Cell ◽  
Leydig Cells ◽  
Interstitial Cell ◽  
Buoyant Density ◽  
Testicular Tissue ◽  
Interstitial Cells ◽  
Adult Rats ◽  
Chain Cleavage ◽  
Igf I ◽  
Cell Fractions

ABSTRACT IGF-I mRNA has been demonstrated in testicular tissue and, more recently, localized specifically to Leydig cells. This study investigated the expression of IGF-I and side-chain cleavage enzyme (SCC) mRNA in two preparations of rat interstitial testicular cells which were separated by buoyant density into Leydig cell-enriched and -depleted fractions. RNA was prepared from interstitial cells obtained from the testes of untreated adult and immature rats and adult rats treated with human chorionic gonadotrophin (hCG) or ethane dimethanesulphonate (EDS; to destroy Leydig cells). IGF-I mRNA was detected in all samples, with five major transcripts ranging from 7·5 to 0·6 kb. Leydig cells (3β-hydroxysteroid dehydrogenase-positive and sensitive to EDS) expressed abundant IGF-I and SCC mRNAs, and levels of both were increased following hCG treatment. However, in addition, IGF-I mRNA which was derived from non-Leydig interstitial cells was detected, in the complete absence of SCC message, either in the more buoyant interstitial cells or in both interstitial cell fractions following the destruction of Leydig cells by EDS treatment. IGF-I expression in the Leydig cell-depleted cell fraction was also increased by hCG treatment, and it is therefore suggested that at least part of this non-Leydig interstitial cell IGF-I mRNA originates in Leydig cell precursors. In conclusion, Leydig cells are not the sole origin of IGF-I mRNA in the testis, and the non-Leydig cell expression may be an important component of testicular IGF-I production.

Increase in Leydig cell responsiveness in the unilaterally cryptorchid rat testis and its relationship to the intratesticular levels of testosterone

1984 ◽  
Vol 102 (3) ◽  
pp. 319-327 ◽  
Author(s):  
R. M. Sharpe ◽  
I. Cooper ◽  
D. G. Doogan
Keyword(s):  
Leydig Cell ◽  
Leydig Cells ◽  
Venous Blood ◽  
Cell Interaction ◽  
Adult Rats ◽  
Similar Reduction ◽  
Testosterone Production ◽  
Lh Releasing Hormone ◽  
Unilateral Cryptorchidism

ABSTRACT Adult rats were made unilaterally cryptorchid (UCD) and 6–7 weeks later Leydig cells were isolated from the scrotal and abdominal testes and their capacity to secrete testosterone in vitro was compared. Basal testosterone production by Leydig cells from the abdominal testes of UCD rats was lowered, compared with cells from the contralateral scrotal testes, whilst their responsiveness to both human chorionic gonadotrophin and an LH releasing hormone agonist was enhanced two- to threefold (P< 0·001) compared both with cells from the contralateral scrotal testes and with cells isolated from untreated rats of the same age. In the UCD rats, concentrations of testosterone in testicular interstitial fluid (IF) were reduced (P< 0·001) by 70–90% in abdominal, compared with scrotal, testes. A similar reduction was evident in the levels of testosterone in spermatic venous blood, and both this decrease and that in IF levels of testosterone varied according to the degree of testicular involution. The ontogeny of the above changes was investigated. After induction of unilateral cryptorchidism, the weight of the abdominal compared with the scrotal testis declined slowly, such that by day 5 there was only a 25% reduction in weight compared with a 70% reduction by day 40. In contrast, the levels of testosterone in IF from abdominal testes declined rapidly, such that by day 5 an 80% reduction was attained, compared with scrotal testes, with little further change by day 40. Hormone-stimulated testosterone production by Leydig cells isolated from the abdominal testes was unchanged or marginally reduced over the first 3 days compared with cells from the scrotal testes, but by day 5 there was a significant increase in responsiveness; this increase was of smaller magnitude than that evident at day 40. These results suggest a possible association between the fall in intratesticular levels of testosterone induced by unilateral cryptorchidism and the Leydig cell hypertrophy and hyper-responsiveness that occurs in the same testes. The implications with respect to altered Sertoli–Leydig cell interaction are discussed. J. Endocr. (1984) 102, 319–327

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