scholarly journals Direct observation of myelination in vivo in the mature human central nervous system. A model for the behaviour of oligodendrocyte progenitors and their progeny

Brain ◽  
1997 ◽  
Vol 120 (11) ◽  
pp. 2071-2082 ◽  
Author(s):  
S. Hunter
Author(s):  
Mehrak Mahmoudi ◽  
Piroz Zamankhan ◽  
William Polashenski

The nervous system remains one of the least understood biological structures due in large part to the enormous complexity of this organ. A theoretical model for the transfer of nerve impulses would be valuable for the analysis of various phenomena in the nervous system, which are difficult to study by experiments. The central nervous system is composed of more than 100 billion neurons, through which information is transmitted via nerve impulses. Nerve impulses are not immediately apparent since each impulse may be blocked during transmission, changed from a single impulse into repetitive impulse, or integrated with impulses from other neurons to form highly intricate patterns. In the human central nervous system, a neuron secretes a chemical substance called a neurotransmitter at the synapse, and this transmitter in turn acts on another neuron to cause excitation, inhibition, or some other modification of its sensitivity.


2012 ◽  
Vol 124 (6) ◽  
pp. 893-903 ◽  
Author(s):  
Pascal F. Durrenberger ◽  
Francisca S. Fernando ◽  
Roberta Magliozzi ◽  
Samira N. Kashefi ◽  
Timothy P. Bonnert ◽  
...  

2010 ◽  
Vol 92 (3) ◽  
pp. 227-244 ◽  
Author(s):  
Jochen De Vry ◽  
Pilar Martínez-Martínez ◽  
Mario Losen ◽  
Yasin Temel ◽  
Thomas Steckler ◽  
...  

2018 ◽  
Author(s):  
Subashika Govindan ◽  
Polina Oberst ◽  
Denis Jabaudon

AbstractThis protocol describes a fluorescence birthdating technique to label, track and isolate isochronic cohorts of newborn cells in the central nervous system in vivo. Injection of carboxyfluorescein esters into the cerebral ventricle allows pulse-labeling of M-phase progenitors in touch with the ventricle and their progeny across the central nervous system, a procedure we termed FlashTag. Labeled cells can be imaged ex vivo or in fixed tissue, targeted for electrophysiological experiments, or isolated using Fluorescence-Activated Cell Sorting (FACS) for cell culture or (single-cell) RNA-sequencing. The dye is retained for several weeks, allowing labeled cells to be identified postnatally. This protocol describes the labeling procedure using in utero injection, the isolation of live cells using FACS, as well as the processing of labeled tissue using immunohistochemistry.


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