scholarly journals In vivo pulse-labeling of isochronic cohorts of cells in the central nervous system using FlashTag

2018 ◽  
Author(s):  
Subashika Govindan ◽  
Polina Oberst ◽  
Denis Jabaudon
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cell Sorting ◽  
Ex Vivo ◽  
Live Cells ◽  
Fixed Tissue ◽  
System A ◽  
M Phase ◽  
The Central Nervous System

AbstractThis protocol describes a fluorescence birthdating technique to label, track and isolate isochronic cohorts of newborn cells in the central nervous system in vivo. Injection of carboxyfluorescein esters into the cerebral ventricle allows pulse-labeling of M-phase progenitors in touch with the ventricle and their progeny across the central nervous system, a procedure we termed FlashTag. Labeled cells can be imaged ex vivo or in fixed tissue, targeted for electrophysiological experiments, or isolated using Fluorescence-Activated Cell Sorting (FACS) for cell culture or (single-cell) RNA-sequencing. The dye is retained for several weeks, allowing labeled cells to be identified postnatally. This protocol describes the labeling procedure using in utero injection, the isolation of live cells using FACS, as well as the processing of labeled tissue using immunohistochemistry.

In vivo electroporation of the central nervous system: A non-viral approach for targeted gene delivery

2010 ◽  
Vol 92 (3) ◽  
pp. 227-244 ◽  
Author(s):  
Jochen De Vry ◽  
Pilar Martínez-Martínez ◽  
Mario Losen ◽  
Yasin Temel ◽  
Thomas Steckler ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Gene Delivery ◽  
In Vivo Electroporation ◽  
Targeted Gene Delivery ◽  
System A ◽  
The Central Nervous System

scholarly journals Nanotechnology in Neurology—Current Status and Future Possibilities

US Neurology ◽  
2010 ◽  
Vol 06 (01) ◽  
pp. 12 ◽  
Author(s):  
James M Provenzale ◽  
Aaron M Mohs ◽  
◽  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Ex Vivo ◽  
Current Status ◽  
Medical Applications ◽  
Nanoparticle Delivery ◽  
New Methods ◽  
The Central Nervous System ◽  
Neuro Protection

The field of nanomedicine is rapidly emerging and will provide many novel methods for diagnosis and treatment. In this article the applications of nanotechnology to the central nervous system (CNS) will be described. Nanotechnology provides many potential solutions to various problems encountered in CNS diseases. Specifically, nanomedicine offers the possibility of new methods of drug delivery, more sensitive and specific means for diagnosis of disease at earlier stages and assessment of treatment response, and also potential techniques for neuro-protection and neuro-engineering. In this article, information is provided on the various types of nanoparticles involved in medical applications, the principles of nanoparticle delivery and targeting, and bothin vivoandex vivouses of nanoscale materials.

scholarly journals Innovative high-resolution microCT imaging of animal brain vasculature

2020 ◽  
Vol 225 (9) ◽  
pp. 2885-2895
Author(s):  
Ruslan Hlushchuk ◽  
David Haberthür ◽  
Petr Soukup ◽  
Sebastien F. Barré ◽  
Oleksiy-Zakhar Khoma ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Dura Mater ◽  
Vertebral Column ◽  
Ex Vivo ◽  
Basic Research ◽  
The Central Nervous System ◽  
Microct Imaging

AbstractAnalysis of the angioarchitecture and quantification of the conduit vessels and microvasculature is of paramount importance for understanding the physiological and pathological processes within the central nervous system (CNS). Most of the available in vivo imaging methods lack penetration depth and/or resolution. Some ex vivo methods may provide better resolution, but are mainly destructive, as they are designed for imaging the CNS tissues after their removal from the skull or vertebral column. The removal procedure inevitably alters the in situ relations of the investigated structures and damages the dura mater and leptomeninges. µAngiofil, a polymer-based contrast agent, permits a qualitatively novel postmortem microangio-computed tomography (microangioCT) approach with excellent resolution and, therefore, visualization of the smallest brain capillaries. The datasets obtained empower a rather straightforward quantitative analysis of the vascular tree, including the microvasculature. The µAngiofil has an excellent filling capacity as well as a radio-opacity higher than the one of bone tissue, which allows imaging the cerebral microvasculature even within the intact skull or vertebral column. This permits in situ visualization and thus investigation of the dura mater and leptomeningeal layers as well as their blood supply in their original geometry. Moreover, the methodology introduced here permits correlative approaches, i.e., microangioCT followed by classical histology, immunohistochemistry and even electron microscopy. The experimental approach presented here makes use of common desktop microCT scanners, rendering it a promising everyday tool for the evaluation of the (micro)vasculature of the central nervous system in preclinical and basic research.

Mills' syndrome. A clinical case of a rare degenerative pathology of the central nervous system

2020 ◽  
pp. 57-60
Author(s):  
Konstantin Gulyabin
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Neurological Diseases ◽  
Cortical Atrophy ◽  
Primary Lateral Sclerosis ◽  
System A ◽  
The Central Nervous System ◽  
Primary Lateral ◽  
Lateral Sclerosis ◽  
Mills Syndrome

Mills' syndrome is a rare neurological disorder. Its nosological nature is currently not completely determined. Nevertheless, Mills' syndrome is considered to be a rare variant of the degenerative pathology of the central nervous system – a variant of focal cortical atrophy. The true prevalence of this pathology is unknown, since this condition is more often of a syndrome type, observed in the clinical picture of a number of neurological diseases (primary lateral sclerosis, frontotemporal dementia, etc.) and is less common in isolated form.

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