scholarly journals Neurofilament and tubulin gene expression in progressive experimental diabetes

Brain ◽  
1999 ◽  
Vol 122 (11) ◽  
pp. 2109-2118 ◽  
Author(s):  
James N. Scott ◽  
Arthur W. Clark ◽  
Douglas W. Zochodne
Keyword(s):  
Gene Expression ◽  
Experimental Diabetes ◽  
Tubulin Gene

Regulation of tubulin gene expression during embryogenesis in drosophila melanogaster

Cell ◽  
1982 ◽  
Vol 28 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Elizabeth C. Raff ◽  
Margaret T. Fuller ◽  
Thomas C. Kaufman ◽  
Kenneth J. Kemphues ◽  
Jane E. Rudolph ◽  
...  
Keyword(s):  
Gene Expression ◽  
Drosophila Melanogaster ◽  
Tubulin Gene

Transcriptional and Post-Transcriptional Control of Tubulin Gene Expression in Trypanosoma cruzi

1992 ◽  
Vol 11 (2) ◽  
pp. 101-109 ◽  
Author(s):  
TURÁN P. ÜRMÉNYI ◽  
FIRMINO T. DE CASTRO ◽  
JOSÉ FRANCISCO O. CARVALHO ◽  
WANDERLEY DE SOUZA ◽  
EDSON RONDINELLI
Keyword(s):  
Gene Expression ◽  
Trypanosoma Cruzi ◽  
Transcriptional Control ◽  
Tubulin Gene

Regulation of oligopeptide transporter (Pept-1) in experimental diabetes

2002 ◽  
Vol 283 (1) ◽  
pp. G133-G138 ◽  
Author(s):  
Archana Gangopadhyay ◽  
Manikkavasagar Thamotharan ◽  
Siamak A. Adibi
Keyword(s):  
Gene Expression ◽  
Intestinal Mucosa ◽  
Brush Border Membrane ◽  
Experimental Diabetes ◽  
Maximal Velocity ◽  
Gene Expressions ◽  
Oligopeptide Transporter ◽  
Uncontrolled Diabetes ◽  
Increased Activity ◽  
Menten Constant

The knowledge of expression and biology of the intestinal oligopeptide transporter (Pept-1) in a metabolic disorder such as diabetes may have nutritional and pharmacological implications. To study this problem, rats were made diabetic by streptozotocin injection, and Western and Northern blot analyses and nuclear run-on assay were used to determine the protein and gene expressions of Pept-1 and its rate of transcription, respectively. Uncontrolled diabetes for 96 h increased the activity of Pept-1 in the brush-border membrane of intestinal mucosa. Studies of Michaelis-Menten constant, maximal velocity, and protein expression of Pept-1 indicated that an increase in the abundance of this transporter was responsible for the increased activity. Studies of the gene expression showed that uncontrolled diabetes increased the abundance of mRNA encoding Pept-1 without altering its rate of transcription. Lastly, studies of the specificity of the above effect showed that uncontrolled diabetes similarly affected the protein and gene expressions of Pept-1 located in the kidney. In conclusion, the data show that 1) uncontrolled diabetes has a tropic effect on Pept-1 and 2) the effect is systemic, and its molecular mechanism appears to be an increase in the stabilization of mRNA encoding Pept-1.

Tubulin gene expression in the Chlamydomonas reinhardtii cell cycle: elimination of environmentally induced artifacts and the measurement of tubulin mRNA levels

1988 ◽  
Vol 89 (3) ◽  
pp. 397-403
Author(s):  
D.S. Nicholl ◽  
J.A. Schloss ◽  
P.C. John
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Cell Division ◽  
Chlamydomonas Reinhardtii ◽  
Environmental Conditions ◽  
Mrna Levels ◽  
Tubulin Gene ◽  
Mrna Accumulation ◽  
Tubulin Mrna ◽  
Flagellar Proteins

To investigate the involvement of tubulin gene expression in controlling cell division events in Chlamydomonas reinhardtii we have measured tubulin mRNA levels during the cell cycle under different environmental conditions. In C. reinhardtii cells grown under the synchronizing conditions of 14 h of light followed by 10 h of darkness, mRNAs for tubulin and associated flagellar proteins were found to accumulate periodically with a peak just prior to cell division. This was not seen when previously synchronized cells were transferred to constant environmental conditions in a turbidostat, suggesting that dramatic changes in tubulin mRNA levels are not required for successful completion of the cell cycle. A hypothesis to explain the patterns of tubulin mRNA accumulation found under different environmental conditions is presented.

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