Bile acids mimic oxidative stress induced upregulation of thioredoxin reductase in colon cancer cell lines

Methanol extracts of five commercially available mushroom species (Phellinus linteus (Berk. et Curt) Teng, Cordyceps sinensis (Berk.) Sacc., Lentinus edodes (Berk.) Pegler, Coprinus comatus (O. F. M?ll.) Pers. and Ganoderma lucidum (Curtis) P. Karst), traditionally used as anticancer agents, were evaluated in vitro for their total phenol and flavonoid contents, cytotoxic and antimigratory activities and antioxidant/prooxidant effects on colon cancer cell lines (HCT-116 and SW-480). Spectrophotometric methods were used for the determination of total phenol content, flavonoid concentrations and DPPH activity of the extracts. Cytotoxic activity was measured by the MTT assay. The antimigratory activity of extracts was determined using the Transwell assay and immunofluorescence staining of ?-catenin. The prooxidant/antioxidant status was followed by measuring the superoxide anion radical (O2?-), nitrite and reduced glutathione (GSH) concentrations. Our results show that the highest phenolic and flavonoid content was found in P. linteus, and its DPPH-scavenging capacity was significantly higher than in other samples. The P. linteus extract significantly decreased cell viability of both tested cancer cell lines. All other extracts selectively inhibited SW-480 cell viability, but did not show significant cytotoxic activity. The mushroom extracts caused changes in the prooxidant/antioxidant status of cells, inducing oxidative stress. All extracts tested on HCT-116 cells demonstrated significant antimigratory effects, which correlated with increased production of O2?- and a reduced level of ?-catenin protein expression, while only P. linteus showed the same effect on SW-480 cells. The results of the present research indicate that the mushroom extracts causes oxidative stress which has a pronounced impact on the migratory status of colon cancer cell lines.

Download Full-text

Arsenic trioxide-induced apoptosis through oxidative stress in cells of colon cancer cell lines

Life Sciences ◽

10.1016/s0024-3205(01)01545-4 ◽

2002 ◽

Vol 70 (19) ◽

pp. 2253-2269 ◽

Cited By ~ 67

Author(s):

Yoshihito Nakagawa ◽

Yukihiro Akao ◽

Hiroshi Morikawa ◽

Ichiro Hirata ◽

Kenichi Katsu ◽

...

Keyword(s):

Oxidative Stress ◽

Colon Cancer ◽

Arsenic Trioxide ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Colon Cancer Cell ◽

Colon Cancer Cell Lines ◽

Induced Apoptosis ◽

In Cells

Download Full-text

Isolation of Cardamonin and Pinostrobin Chalcone from the Rhizomes of Boesenbergia rotunda (L.) Mansf. and their Cytotoxic Effects on H-29 and MDA-MB-231 Cancer Cell Lines

The Natural Products Journal ◽

10.2174/2210315509666190117151542 ◽

2019 ◽

Vol 9 (4) ◽

pp. 341-348 ◽

Cited By ~ 4

Author(s):

Ibrahim Awad Mohammed ◽

Muhammad Nadeem Akhtar ◽

Foo Jhi Biau ◽

Yin Sim Tor ◽

Seema Zareen ◽

...

Keyword(s):

Breast Cancer ◽

Colon Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Chemical Constituents ◽

Cancer Cell Lines ◽

Colon Cancer Cell ◽

Colon Cancer Cell Lines ◽

Ht 29

Background: Breast cancer and human colon cancer are the most common types of cancer in females and males, respectively. Breast cancer is the most common type of cancer after lung and colon cancers. Natural products are an important source for drug discovery. Boesenbergia rotunda (L.) Mansf. is commonly known as finger root, belonging to the Zingiberaceae family. Objective: The aim of this study to isolate some natural compounds from the rhizomes of B. rotunda (L.) Mansf., and to investigate their cytotoxicity against the human triple-negative breast cancer cell (MDA-MB-231) and HT-29 colon cancer cell lines. Methods: The dried rhizomes of B. rotunda were extracted with methanol. The methanolic extract was further used for solvent-solvent extraction. Bioassay-guided extraction and isolation of the rhizomes of the B. rotunda exhibited cytotoxic properties of hexane and dichloromethane fractions. Results: Six major chemical constituents, pinostrobin (1), pinostrobin chalcone (2), cardamonin (3), 4,5-dihydrokawain (4), pinocembrin (5), and alpinetin (6) were isolated from the rhizomes of the B. rotunda. All the chemical constituents were screened against the human triple-negative breast cancer cell (MDA-MB-231) and HT-29 colon cancer cell lines. The compound cardamonin (3) (IC50 = 5.62±0.61 and 4.44±0.66 µg/mL) and pinostrobin chalcone (2), (IC50 = 20.42±2.23 and 22.51±0.42 μg/mL) were found to be potent natural cytotoxic compounds against MDA-MB-231 and HT-29 colon cancer cell lines, respectively. Conclusion: Cardamonin (3) and pinostrobin chalcone (2) were found to be the most potential natural compounds against breast cancer cell line MDA-MB-231 and colon cancer HT-29 cell line.

Download Full-text

Reactivity of Monoclonal Antibodies Directed against Lung Cancer Antigens with Human Lung, Breast and Colon Cancer Cell Lines

Disease Markers ◽

10.1155/1993/619781 ◽

1993 ◽

Vol 11 (5-6) ◽

pp. 225-237

Author(s):

Udo Schumacher ◽

Dhia Mukthar ◽

Thomas Schenker

Keyword(s):

Breast Cancer ◽

Lung Cancer ◽

Colon Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Colon Cancer Cell ◽

Breast Cancer Cell Lines ◽

Cancer Antigens ◽

Colon Cancer Cell Lines

A panel of monoclonal antibodies (n=72 including controls) directed against lung cancer antigens was screened immunohistochemically against a panel of seven human lung cancer cell lines (including small cell carcinoma, squamous cell carcinoma, adenocarcinoma and mesothelioma), six human breast cancer cell lines and one human colon cancer cell line, The majority of the antibodies (n=42) reacted also with antigens present on breast and colon cancer cell lines, This cross reactivity especially between lung and breast cancer cell lines is not altogether unexpected since antigens common to breast and lung tissue including their neoplasms such as MUC1 antigen have been described, Our results indicate that epitopes shared by lung and breast cancers are probably more common than previously thought. The relevance for prognosis and therapy of these shared antigens, especially as disease markers in breast cancer, has to be investigated.

Download Full-text

CRIF1 enhances p53 activity via the chromatin remodeler SNF5 in the HCT116 colon cancer cell lines

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms ◽

10.1016/j.bbagrm.2017.02.006 ◽

2017 ◽

Vol 1860 (4) ◽

pp. 516-522 ◽

Cited By ~ 4

Author(s):

Hai-xia Yan ◽

Yan-jun Zhang ◽

Yuan Zhang ◽

Xue Ren ◽

Yu-fei Shen ◽

...

Keyword(s):

Colon Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Colon Cancer Cell ◽

Chromatin Remodeler ◽

Hct116 Colon Cancer Cell ◽

Colon Cancer Cell Lines

Download Full-text

Antitumour activity of XR5944 in vitro and in vivo in combination with 5-fluorouracil and irinotecan in colon cancer cell lines

British Journal of Cancer ◽

10.1038/sj.bjc.6602403 ◽

2005 ◽

Vol 92 (4) ◽

pp. 722-728 ◽

Cited By ~ 17

Author(s):

S M Harris ◽

P Mistry ◽

C Freathy ◽

J L Brown ◽

P A Charlton

Keyword(s):

Colon Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Antitumour Activity ◽

Cancer Cell Lines ◽

Colon Cancer Cell ◽

Colon Cancer Cell Lines

Download Full-text

Efficiency of brown seaweed (Sargassum longifolium) polysaccharides encapsulated in nanoemulsion and nanostructured lipid carrier against colon cancer cell lines HCT 116

RSC Advances ◽

10.1039/c8ra02616e ◽

2018 ◽

Vol 8 (29) ◽

pp. 15973-15984 ◽

Cited By ~ 16

Author(s):

Saghya Infant Shofia ◽

Kannan Jayakumar ◽

Amitava Mukherjee ◽

Natarajan Chandrasekaran

Keyword(s):

Colon Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Brown Seaweed ◽

Cancer Cell Lines ◽

Colon Cancer Cell ◽

Nanostructured Lipid Carrier ◽

Brown Seaweeds ◽

Colon Cancer Cell Lines ◽

Hct 116

Bioactive polysaccharides extracted from brown seaweeds have potent antioxidant, antitumor, antibacterial, antiviral, anti-inflammatory activities and nanomedicine applications.

Download Full-text

β-Lapachone Inhibits Lung Metastasis of Colorectal Cancer by Inducing Apoptosis of CT26 Cells

Integrative Cancer Therapies ◽

10.1177/1534735416681638 ◽

2016 ◽

Vol 16 (4) ◽

pp. 585-596 ◽

Cited By ~ 6

Author(s):

Ji-Ye Kee ◽

Yo-Han Han ◽

Jinbong Park ◽

Dae-Seung Kim ◽

Jeong-Geon Mun ◽

...

Keyword(s):

Cell Cycle ◽

Colon Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Lung Metastasis ◽

Cancer Cell Lines ◽

Colon Cancer Cell ◽

Experimental Metastasis ◽

Colon Cancer Cell Lines ◽

Metastasis Model

Background: β-Lapachone is a quinone-containing compound found in red lapacho ( Tabebuia impetiginosa, syn. T avellanedae) trees. Lapacho has been used in traditional medicine by several South and Central American indigenous people to treat various types of cancer. The purpose of this study was to investigate the antimetastatic properties of β-lapachone and the underlying mechanisms using colon cancer cells. Methods: This research used metastatic murine colon cancer cell lines, colon 26 (CT26) and colon 38 (MC38). A WST assay, annexin V assay, cell cycle analysis, wound healing assay, invasion assay, western blot analysis, and real-time reverse transcription–polymerase chain reaction were performed to examine the effects of β-lapachone on metastatic phenotypes and molecular mechanisms. The effect of β-lapachone on lung metastasis was assessed in a mouse experimental metastasis model. Results: We found that the inhibition of proliferation of the colon cancer cell lines by β-lapachone was due to the induction of apoptosis and cell cycle arrest. β-Lapachone induced the apoptosis of CT26 cells through caspase-3, -8, and -9 activation; poly(ADP-ribose) polymerase cleavage; and downregulation of the Bcl-2 family in a dose- and time-dependent manner. In addition, a low concentration of β-lapachone decreased the cell migration and invasion by decreasing the expression of matrix metalloproteinases-2 and -9, and increased the expression of tissue inhibitors of metalloproteinases-1 and -2. Moreover, β-lapachone treatment regulated the expression of epithelial-mesenchymal transition markers such as E- and N-cadherin, vimentin, β-catenin, and Snail in CT26 cells. In the mouse experimental metastasis model, β-lapachone significantly inhibited the lung metastasis of CT26 cells. Conclusions: Our results demonstrated the inhibitory effect of β-lapachone on colorectal lung metastasis. This compound may be useful for developing therapeutic agents to treat metastatic cancer.

Download Full-text