A Pilot Comparative Pharmacokinetic Study on Mango Polyphenols After Acute Intake of Fresh and Individual Quick Frozen Mango Pulp in Healthy Human Subjects

Abstract Objectives The objective of this study was to characterize and compare the pharmacokinetic profile of polyphenolic metabolites after acute intake of fresh and individually quick frozen (IQF) mango pulp in human plasma over 24 hours. Methods Healthy human subjects (n = 7, age = 31 ± 3 years, BMI = 22.7 ± 0.6 kg/m2) received IQF or fresh mango pulp (500 g) or control (100 mg vitamin C) in random order after 3-day low polyphenol diet in a cross over study design. Polyphenols in mango pulps (fresh and IQF) and their metabolites were characterized in plasma collected before (fasting, baseline) and at 30 min or hourly intervals for 8 h then again at 24 h using ultra high performance liquid chromatography and mass spectrometry (UHPLC-MS). Multiple comparisons within and among treatments (IQF and Fresh) over 24 h postprandial time were performed by mixed model using SAS with statistical significance set at P < 0.05. Results Gallic acid (228.1 ± 7.3 µg/100 g fresh weight, FW) and galloyl glucose (5.0 ± 0.2 µg/100 FW) were significantly higher (P < 0.05) in fresh compared to IQF mango pulp. In plasma however, gallic acid (Cmax = 17.3 ± 4.0 nmol/L, AUC0–24h = 63.9 ± 7.9 nmol × h/L), galloyl glucose (Cmax = 12.4 ± 2.3 nmol/L, AUC0–24h = 49.2 ± 8.1 nmol × h/L), ferulic acid hexoside (Cmax = 2.8 ± 0.5 nmol/L, AUC0–24h = 8.6 ± 1.9 nmol × h/L), and gallic acid and pyrogallol metabolites (sulfates, methyl and glucuronides) were significantly higher after IQF mango pulp intake compared to fresh mango pulp as assessed by maximum concentrations achieved (Cmax) and area under the curve (AUC0–24 h). Conclusions These results indicate that IQF may preserve or enhance the bio-accessibility of mango (poly)phenol components compared to fresh mangoes, which are hot water treated before importing into the USA. Funding Sources This study was funded by the National Mango Board.

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Chronic stress decreases circulating endocannabinoid 2-arachidonoylglycerol in healthy human subjects

Endocrine Abstracts ◽

10.1530/endoabs.37.gp.18.01 ◽

2015 ◽

Author(s):

Buqing Yi ◽

Igor Nichiporuk ◽

Matthias Feuerecker ◽

Gustav Schelling ◽

Alexander Chouker

Keyword(s):

Chronic Stress ◽

Human Subjects ◽

Healthy Human ◽

Healthy Human Subjects

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Effect of a Novel Ficus Carica (Fig) Fruit Extract Standardized in Abscisic Acid on the Glycemic and Insulinemic Responses in Healthy Human Subjects

Diabetes ◽

10.2337/db18-791-p ◽

2018 ◽

Vol 67 (Supplement 1) ◽

pp. 791-P

Author(s):

ANDREA ZANGARA

Keyword(s):

Abscisic Acid ◽

Human Subjects ◽

Ficus Carica ◽

Fruit Extract ◽

Healthy Human ◽

Healthy Human Subjects

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Plasma clearance of chylomicrons labeled with retinyl palmitate in healthy human subjects.

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)37744-0 ◽

1984 ◽

Vol 25 (8) ◽

pp. 805-812

Author(s):

F Berr ◽

F Kern

Keyword(s):

Plasma Clearance ◽

Human Subjects ◽

Retinyl Palmitate ◽

Healthy Human ◽

Healthy Human Subjects

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Steady-state kinetics of serum bile acids in healthy human subjects: single and dual isotope techniques using stable isotopes and mass spectrometry.

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)38702-2 ◽

1987 ◽

Vol 28 (3) ◽

pp. 238-252

Author(s):

G T Everson

Keyword(s):

Mass Spectrometry ◽

Stable Isotopes ◽

Steady State ◽

Bile Acids ◽

Human Subjects ◽

Healthy Human ◽

Dual Isotope ◽

Steady State Kinetics ◽

Healthy Human Subjects ◽

Kinetics Of

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Acacia Gum Is Well Tolerated While Increasing Satiety and Lowering Peak Blood Glucose Response in Healthy Human Subjects

Nutrients ◽

10.3390/nu13020618 ◽

2021 ◽

Vol 13 (2) ◽

pp. 618

Author(s):

Riley Larson ◽

Courtney Nelson ◽

Renee Korczak ◽

Holly Willis ◽

Jennifer Erickson ◽

...

Keyword(s):

Blood Glucose ◽

Human Subjects ◽

Area Under The Curve ◽

Soluble Fiber ◽

Healthy Human ◽

Glucose Response ◽

Blood Glucose Response ◽

Acacia Gum ◽

Fiber Treatment ◽

Healthy Human Subjects

Acacia gum (AG) is a non-viscous soluble fiber that is easily incorporated into beverages and foods. To determine its physiological effects in healthy human subjects, we fed 0, 20, and 40 g of acacia gum in orange juice along with a bagel and cream cheese after a 12 h fast and compared satiety, glycemic response, gastrointestinal tolerance, and food intake among treatments. Subjects (n = 48) reported less hunger and greater fullness at 15 min (p = 0.019 and 0.003, respectively) and 240 min (p = 0.036 and 0.05, respectively) after breakfast with the 40 g fiber treatment. They also reported being more satisfied at 15 min (p = 0.011) and less hungry with the 40 g fiber treatment at 30 min (p = 0.012). Subjects reported more bloating, flatulence, and GI rumbling on the 40 g fiber treatment compared to control, although values for GI tolerance were all low with AG treatment. No significant differences were found in area under the curve (AUC) or change from baseline for blood glucose response, although actual blood glucose with 20 g fiber at 30 min was significantly less than control. Individuals varied greatly in their postprandial glucose response to all treatments. AG improves satiety response and may lower peak glucose response at certain timepoints, and it is well tolerated in healthy human subjects. AG can be added to beverages and foods in doses that can help meet fiber recommendations.

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