Determination of Serum Calcium by Automated Atomic Absorption Spectroscopy

1967 ◽  
Vol 13 (5) ◽  
pp. 388-396 ◽  
Author(s):  
Bernard Klein ◽  
James H Kaufman ◽  
Stanley Morgenstern
Keyword(s):  
Hydrochloric Acid ◽  
Atomic Absorption ◽  
Serum Calcium ◽  
Flow System ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry ◽  
Comparison Of The Results ◽  
Automated Determination ◽  
Good Agreement

Abstract A procedure is presented for the automated determination of serum calcium by atomic absorption spectrophotometry. The serum sample, diluted with acidified lanthanum dichloride, is dialyzed against 0.1 N hydrochloric acid and a portion of the recipient solution is pumped into the atomizer-burner of the spectrophotometer. Data are presented on the precision and reproducibility of the flow system. Addition of calcium to serum shows a mean recovery of 99%. A comparison of the results of analyses on random specimens by permanganate titration and the automated procedure shows good agreement.

Automated Atomic Absorption Spectrophotometry

1967 ◽  
Vol 13 (9) ◽  
pp. 788-796 ◽  
Author(s):  
Bernard Klein ◽  
James H Kaufman ◽  
Morris Oklander
Keyword(s):  
Atomic Absorption ◽  
Excellent Agreement ◽  
Serum Calcium ◽  
Flow System ◽  
Serum Magnesium ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry ◽  
Present Procedure ◽  
Comparison Of The Results

Abstract The flow system developed for the determination of serum calcium by automated atomic absorption spectrophotometry (AAS) was adapted to the determination of serum magnesium. A comparison of the results of analyses by the present procedure and by an automated fluorometric procedure on identical serum specimens shows excellent agreement.

Automated Atomic Absorption Spectrophotometry

1967 ◽  
Vol 13 (9) ◽  
pp. 797-805 ◽  
Author(s):  
Bernard Klein ◽  
James H Kaufman ◽  
Morris Oklander
Keyword(s):  
Atomic Absorption ◽  
Flow System ◽  
Atomic Absorption Spectrophotometry ◽  
Spinal Fluid ◽  
Absorption Spectrophotometry ◽  
Comparison Of Results ◽  
Urine Specimens ◽  
Good Agreement

Abstract A simplified flow system is presented for the determination of calcium in urine and spinal fluid, by automated atomic absorption spectrophotometry (AAS). Analysis following the addition of calcium to urine or spinal fluid specimens showed a mean recovery of 103% and 99%, respectively. A comparison of results of analyses on unselected urine specimens by manual and automated AAS shows good agreement.

Automated Atomic Absorption Spectrophotometry

1967 ◽  
Vol 13 (12) ◽  
pp. 1079-1087 ◽  
Author(s):  
Bernard Klein ◽  
James H Kaufman
Keyword(s):  
Atomic Absorption ◽  
Simultaneous Determination ◽  
Serum Calcium ◽  
Flow System ◽  
Atomic Absorption Spectrophotometry ◽  
Reducing Agent ◽  
Molybdenum Blue ◽  
Absorption Spectrophotometry ◽  
Blue Reaction

Abstract A procedure is described for the automated simultaneous determination of serum calcium and phosphate. Calcium is assayed by atomic absorption spectrophotometry. Phosphate is determined by the molybdenum blue reaction in an improved flow system with a more sensitive reducing agent.

Biological Conjugate between Antibody and ZnS Nanoparticles as Probe for Atomic Absorption Spectrophotometry Determination of Estriol

2014 ◽  
Vol 556-562 ◽  
pp. 64-66
Author(s):  
Chun Yan Zhang ◽  
Chuan Tao Wang ◽  
Shu Hao Wang ◽  
Ling Yun Du
Keyword(s):  
Atomic Absorption ◽  
Limit Of Detection ◽  
Water Solution ◽  
Semiconductor Nanocrystals ◽  
Atomic Absorption Spectrophotometry ◽  
Serum Samples ◽  
Absorption Spectrophotometry ◽  
Primary Materials ◽  
Good Agreement

ZnS semiconductor nanocrystals (NCs) were prepared by ways from primary materials of ZnCl2 and Na2S in water solution. Using the synthesized ZnS NCs, a polyclonal antibody-based ZnS-labelled immunosorbent assay for the determination of estriol (E3) was developed with atomic absorption spectrophotometry (AAS) as a detector. An immunoaffinity column was applied to testify conjugation between antibody and ZnS NCs. The linear range for determination of estriol is 40.0~600.0 ng.mL-1, and the limit of detection (LOD) is 10.0 ng.mL-1. Some serum samples have been analyzed with satisfactory results which are in good agreement with those obtained using ELISA. This work suggests the potential application of NCs as biological probes and AAS as detector in nonisotopic immunoassay.

Application of Atomic Absorption Spectrophotometry in the Determination of Calcium in Serum

1964 ◽  
Vol 10 (10) ◽  
pp. 869-890 ◽  
Author(s):  
Alfred Zettner ◽  
David Seligson
Keyword(s):  
Calcium Oxalate ◽  
Atomic Absorption ◽  
Serum Calcium ◽  
Atomic Absorption Spectrophotometry ◽  
Dilution Technique ◽  
Serum Samples ◽  
Absorption Spectrophotometry

Abstract Atomic absorption spectrophotometry (AAS) was applied to the determination of serum calcium. A special diluent was developed to abolish the effect of absorption depressors. This made possible measurement of calcium in directly diluted serum samples without prior removal of any serum constituents. Values obtained by AAS on calcium oxalate precipitates from serum were identical with those found by the direct dilution technique.

Innovations in Atomic Absorption Spectrophotometry with Electrothermal Atomization for Determining Lead in Foods

1982 ◽  
Vol 65 (4) ◽  
pp. 994-998
Author(s):  
Theodore C Rains ◽  
Theresa A Rush ◽  
Therese A Butler
Keyword(s):  
Atomic Absorption ◽  
Ammonium Phosphate ◽  
Atomic Absorption Spectrophotometry ◽  
Oyster Tissue ◽  
Absorption Spectrophotometry ◽  
Matrix Modification ◽  
Precision And Accuracy ◽  
Certified Values ◽  
Good Agreement

Abstract A simple and rapid method is described for the determination of lead in foods. The samples are digested in HNO3, HF, and HCIO4 and then the lead is determined by atomic absorption spectrophometry using an electrothermal atomizer with the L'vov platform. Interferences and ways to improve the precision and accuracy of the analysis were studied. Matrix modification using 1% ammonium phosphate alleviated most interferences encountered. The precision and accuracy of the method was evaluated using NBS SRM 1570 Spinach and SRM 1566 Oyster Tissue. The values obtained are in good agreement with the certified values.

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