Application of Atomic Absorption Spectrophotometry in the Determination of Calcium in Serum

1964 ◽  
Vol 10 (10) ◽  
pp. 869-890 ◽  
Author(s):  
Alfred Zettner ◽  
David Seligson
Keyword(s):  
Calcium Oxalate ◽  
Atomic Absorption ◽  
Serum Calcium ◽  
Atomic Absorption Spectrophotometry ◽  
Dilution Technique ◽  
Serum Samples ◽  
Absorption Spectrophotometry

Abstract Atomic absorption spectrophotometry (AAS) was applied to the determination of serum calcium. A special diluent was developed to abolish the effect of absorption depressors. This made possible measurement of calcium in directly diluted serum samples without prior removal of any serum constituents. Values obtained by AAS on calcium oxalate precipitates from serum were identical with those found by the direct dilution technique.

Biological Conjugate between Antibody and ZnS Nanoparticles as Probe for Atomic Absorption Spectrophotometry Determination of Estriol

2014 ◽  
Vol 556-562 ◽  
pp. 64-66
Author(s):  
Chun Yan Zhang ◽  
Chuan Tao Wang ◽  
Shu Hao Wang ◽  
Ling Yun Du
Keyword(s):  
Atomic Absorption ◽  
Limit Of Detection ◽  
Water Solution ◽  
Semiconductor Nanocrystals ◽  
Atomic Absorption Spectrophotometry ◽  
Serum Samples ◽  
Absorption Spectrophotometry ◽  
Primary Materials ◽  
Good Agreement

ZnS semiconductor nanocrystals (NCs) were prepared by ways from primary materials of ZnCl2 and Na2S in water solution. Using the synthesized ZnS NCs, a polyclonal antibody-based ZnS-labelled immunosorbent assay for the determination of estriol (E3) was developed with atomic absorption spectrophotometry (AAS) as a detector. An immunoaffinity column was applied to testify conjugation between antibody and ZnS NCs. The linear range for determination of estriol is 40.0~600.0 ng.mL-1, and the limit of detection (LOD) is 10.0 ng.mL-1. Some serum samples have been analyzed with satisfactory results which are in good agreement with those obtained using ELISA. This work suggests the potential application of NCs as biological probes and AAS as detector in nonisotopic immunoassay.

Determination of Serum Calcium by Automated Atomic Absorption Spectroscopy

1967 ◽  
Vol 13 (5) ◽  
pp. 388-396 ◽  
Author(s):  
Bernard Klein ◽  
James H Kaufman ◽  
Stanley Morgenstern
Keyword(s):  
Hydrochloric Acid ◽  
Atomic Absorption ◽  
Serum Calcium ◽  
Flow System ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry ◽  
Comparison Of The Results ◽  
Automated Determination ◽  
Good Agreement

Abstract A procedure is presented for the automated determination of serum calcium by atomic absorption spectrophotometry. The serum sample, diluted with acidified lanthanum dichloride, is dialyzed against 0.1 N hydrochloric acid and a portion of the recipient solution is pumped into the atomizer-burner of the spectrophotometer. Data are presented on the precision and reproducibility of the flow system. Addition of calcium to serum shows a mean recovery of 99%. A comparison of the results of analyses on random specimens by permanganate titration and the automated procedure shows good agreement.

Automated Atomic Absorption Spectrophotometry

1967 ◽  
Vol 13 (9) ◽  
pp. 788-796 ◽  
Author(s):  
Bernard Klein ◽  
James H Kaufman ◽  
Morris Oklander
Keyword(s):  
Atomic Absorption ◽  
Excellent Agreement ◽  
Serum Calcium ◽  
Flow System ◽  
Serum Magnesium ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry ◽  
Present Procedure ◽  
Comparison Of The Results

Abstract The flow system developed for the determination of serum calcium by automated atomic absorption spectrophotometry (AAS) was adapted to the determination of serum magnesium. A comparison of the results of analyses by the present procedure and by an automated fluorometric procedure on identical serum specimens shows excellent agreement.

Automated Method for Determination of Serum Calcium by Use of Alizarin

1970 ◽  
Vol 16 (10) ◽  
pp. 816-819 ◽  
Author(s):  
Christopher S Frings ◽  
Patricia S Cohen ◽  
Lowell B Foster
Keyword(s):  
Atomic Absorption ◽  
Serum Calcium ◽  
Coefficient Of Variation ◽  
Atomic Absorption Spectrophotometry ◽  
Manual Method ◽  
Absorption Spectrophotometry ◽  
Automated Method ◽  
Beer's Law ◽  
Beer’S Law

Abstract An automated method is described for determining serum calcium by measuring the red chromogenic complex formed by calcium and alizarin. About 40 µl of undialyzed serum is added directly to one reagent to pro-duce the color, which follows Beer's law at 599 nm to a concentration of 15.0 mg of calcium per 100 ml, with a coefficient of variation of 2.5%. Serum calcium concentrations, as measured by our method, compare favorably with those by the corresponding manual method and by atomic absorption spectrophotometry

Automated Atomic Absorption Spectrophotometry

1967 ◽  
Vol 13 (12) ◽  
pp. 1079-1087 ◽  
Author(s):  
Bernard Klein ◽  
James H Kaufman
Keyword(s):  
Atomic Absorption ◽  
Simultaneous Determination ◽  
Serum Calcium ◽  
Flow System ◽  
Atomic Absorption Spectrophotometry ◽  
Reducing Agent ◽  
Molybdenum Blue ◽  
Absorption Spectrophotometry ◽  
Blue Reaction

Abstract A procedure is described for the automated simultaneous determination of serum calcium and phosphate. Calcium is assayed by atomic absorption spectrophotometry. Phosphate is determined by the molybdenum blue reaction in an improved flow system with a more sensitive reducing agent.

Determination of Minor Nutrients in Fertilizers by Atomic Absorption Spectrophotometry: Second Collaborative Study

1965 ◽  
Vol 48 (6) ◽  
pp. 1100-1103
Author(s):  
C H Mcbride
Keyword(s):  
Atomic Absorption ◽  
Collaborative Study ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Method ◽  
Atomic Absorption Method ◽  
Absorption Spectrophotometry ◽  
Minor Nutrients

Abstract The atomic absorption method studied last year was re-examined and extended to include calcium and sodium. The procedures were submitted to 16 collaborators for determination of Ca, Cu, Fe, Mg, Mn, Na, and Zn. Results for Ca and Na were discouraging; further study is recommended.

Comparison of Methods for Determining Biuret in Urea

1979 ◽  
Vol 62 (1) ◽  
pp. 153-159
Author(s):  
Luis F Corominas ◽  
Victor M Boy ◽  
Manuel Guijosa
Keyword(s):  
Atomic Absorption ◽  
Spectrophotometric Determination ◽  
Atomic Absorption Spectrophotometry ◽  
International Organization ◽  
Comparison Of Methods ◽  
Definite Advantage ◽  
Absorption Spectrophotometry ◽  
Alternative Procedures ◽  
Aoac Method

Abstract The official first action AOAC method for the spectrophotometric determination of biuret in urea, 2.072—2.074, was compared with official first action AOAC method 2.C01-2.C03 (atomic absorption spectrophotometry), 2 simplified versions of 2.072-2.074, and modified versions of 2 alternative procedures of the International Organization for Standardization. Three synthetic urea samples (0.3, 1.4, and 3.0% biuret) and 1 commercial urea sample (1.0% biuret) were analyzed. The methods proved to be equivalent and none showed a definite advantage over 2.072-2.074. The purification of biuret and the interference by ammonia are also discussed.

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