Creatine kinase isoenzymes in baboon tissues and organs.

1976 ◽  
Vol 22 (1) ◽  
pp. 63-66 ◽  
Author(s):  
W G Yasmineh ◽  
R B Pyle ◽  
N Q Hanson ◽  
B K Hultman
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Gastrointestinal Tract ◽  
Bile Duct ◽  
Common Bile Duct ◽  
Gall Bladder ◽  
Total Activity ◽  
Creatine Kinase Isoenzymes ◽  
Wet Weight

Abstract We determined the activity of the isoenzymes of creatine kinase in homogenates of fresh tissues from six baboons. Twenty-six tissues were removed from each baboon and the isoenzymes in them were separated by chromatography on diethylaminoethyl-Sephadex A-50. Total activity was greatest in skeletal muscle (2600 U/g wet weight) followed by myocardium (800 U/g), brain (180 U/g), and the bladder and tissues of the gastrointestinal tract (about 100 U/g). Ten tissues had total activities of less than 20 U/g: trachea, lung, kidney, thyroid, gall bladder, liver, spleen, pancreas, common bile duct, and adrenal. Skeletal muscle contained only the MM isoenzyme; myocardium contained 81% MM isoenzyme, 18% MB isoenzyme, and 1% BB isoenzyme. Except for thyroid and pancreas, all other tissues contained mainly the BB isoenzyme. Tissues of the gastrointestinal tract contained significant (7 to 17%) MB isoenzyme activity.

Cytoplasmic creatine kinase isoenzymes quantitated in tissue specimens obtained at surgery.

1983 ◽  
Vol 29 (2) ◽  
pp. 310-313 ◽  
Author(s):  
P Urdal ◽  
K Urdal ◽  
J H Strømme
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Anion Exchange ◽  
Creatine Kinase Isoenzymes ◽  
Wet Weight ◽  
Specific Activities ◽  
Tissue Specimens ◽  
Different Tissues

Abstract Because previous reports have given inconsistent results, we re-examined the catalytic concentrations of cytoplasmic creatine kinase (CK) and of CK isoenzymes in 38 biopsies obtained from 19 different tissues. After homogenization and centrifugation many tissues showed high CK catalytic concentrations; 11 of them contained activity exceeding 50 U/g wet weight (Scandinavian recommended method). The highest specific activities were found in skeletal muscle (2400 U/g), brain (530 U/g), and myocardium (460 U/g). The separate isoenzyme activities were estimated by electrophoretic, anion-exchange chromatographic, immunoinhibiting, and radioimmunological methods. CK-BB was present in all tissues and, in fact, was the only cytoplasmic CK isoenzyme in 16 of the 19 tissues examined. CK-MM was the major isoenzyme of skeletal muscle and myocardium and was in addition observed in placenta, in trace amounts. CK-MB was present in high catalytic concentrations in myocardium (20% of total CK) and in low catalytic concentrations in skeletal muscle (1.1% of total CK).

Creatine kinase isoenzymes in cerebrospinal fluid in a case of brain damage.

1976 ◽  
Vol 22 (8) ◽  
pp. 1405-1407 ◽  
Author(s):  
P M Bayer ◽  
F Gabl ◽  
G Granditsch ◽  
K Widhalm ◽  
H Zyman ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Creatine Kinase ◽  
High Activity ◽  
Brain Damage ◽  
Acute Phase ◽  
Clinical Importance ◽  
Total Activity ◽  
Spinal Fluid ◽  
Consumption Coagulopathy ◽  
Creatine Kinase Isoenzymes

Abstract We present a case of a 11/2-year-old boy with toxic enteritis, consecutive consumption coagulopathy, and sever brain damage. During the acute phase we found high activity of the BB isoenzyme of creatine kinase in cerebrospinal fluid, but not in the serum. Isoenzyme MM could also be found in the spinal fluid (37.9% of the total activity). We conclude that analysis for creatine kinase isoenzymes in spinal fluid is of clinical importance.

Creatine kinase isoenzymes of mitochondrial origin in human serum.

1979 ◽  
Vol 25 (6) ◽  
pp. 943-947 ◽  
Author(s):  
G P James ◽  
R L Harrison
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Serum Samples ◽  
Muscle Creatine Kinase ◽  
Cellulose Acetate Electrophoresis ◽  
University Of Texas ◽  
Creatine Kinase Isoenzymes ◽  
Mitochondrial Origin ◽  
Muscle Creatine

Abstract We measured creatine kinase (EC 2.7.3.2) activity in 1009 serum samples from 538 patients in the intensive-care units of the University of Texas Medical Branch hospitals. Creatine kinase isoenzymes migrating cathodal to skeletal muscle creatine kinase (CK-MM) on cellulose acetate electrophoresis were found in sera from 14 of the 538 patients. Creatine kinase, lactate dehydrogenase (EC 1.1.1.27), aspartate aminotransferase (EC 2.6.1.1), and alanine aminotransferase (EC 2.6.1.2) activities were abnormally increased in these 14 patients. Liver lactate dehydrogenase isoenzyme (LDH5) and cardiac creatine kinase isoenzyme (CK-MB) were abnormally increased in 12 and eight of these patients, respectively. Ten of the 14 patients died during their hospital admission. We believe the creatine kinase isoenzymes that migrated cathodal to skeletal muscle creatine kinase (CK-MM) were of mitochondrial origin.

scholarly journals Hepatolitiasis: Kasus Serial di RSUD Dr. Soetomo

2018 ◽  
Vol 2 (1) ◽  
pp. 5 ◽  
Author(s):  
Made Mahayasa ◽  
Tommy Lesmana
Keyword(s):  
Bile Duct ◽  
Magnetic Resonance ◽  
Common Bile Duct ◽  
Gall Bladder ◽  
Hepatic Duct ◽  
Magnetic Resonance Cholangiopancreatography ◽  
Common Hepatic Duct ◽  
Hepatic Bile ◽  
Hepatic Bile Duct

Latar Belakang: hepatolitiasis adalah batu empedu pada saluran empedu liver dengan insidensi 20-30% dari semua pasien yang menjalani operasi untuk penyakit batu empedu. Ada beberapa pilihan operasi hepatolitiasis, seperti hepatektomi, eksplorasi common bile duct (CBD), dan drainase saluran intrahepatik atau cholangioenterostomy (access loop procedures), dan teknik perkutaneus. Pada laporan kasus serial ini, akan dibahas aspek pemilihan operasi pada pasien dengan hepatolitiasis. Kasus: kasus pertama adalah laki-laki, 60 tahun, dirawat di Rumah Sakit Dr. Soetomo dengan nyeri abdomen kuadran kanan atas sejak 2 minggu. Diagnosis dengan USG (ultrasonografi) abdomen dan MRCP (magnetic resonance cholangiopancreatography) menunjukkan terdapat beberapa batu di IHBD (intra hepatic bile duct), CHD (common hepatic duct), CBD, GB (gall bladder), dan sistem bilier yang melebar. Pada pasien dilakukan tindakan kolesistektomi, eksplorasi duktus, dan by pass bilio-digestive Roux en Y (access loop procedures). Kasus kedua adalah perempuan, 45 tahun, dirawat di Rumah Sakit Dr. Soetomo dengan didiagnosis batu IHBD dan CBD. Penderita telah dilakukan kolesistektomi sejak 12 tahun yang lalu. Durante operasi ditemukan atrofi lobus kiri hati. Pada pasien, dilakukan operasi dengan eksplorasi duktus, by pass bilio-digestive Roux en Y (access loop procedures), dan hepatektomi lobus kiri. Simpulan: kasus hepatolitiasis jarang terjadi di Rumah Sakit Dr. Soetomo Surabaya. Diagnosis lengkap memerlukan kombinasi modalitas pencitraan. Pembedahan tetap menjadi pilihan utama pengobatan definitif. Menurut strategi terapeutik saat ini untuk hepatolitiasis, hepatektomi tampaknya merupakan pengobatan yang paling efektif untuk pasien dengan hepatolitiasis kiri yang terisolasi jika prosedur pembedahan lain tidak dapat mengatasi semua lesi terkait. Perawatan yang baik dapat memberikan luaran yang baik.

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