Radioimmunoassay, enzyme immunoassay, spectrophotometry, and gas-liquid chromatography compared for determination of phenobarbital and diphenylhydantoin.

1976 ◽  
Vol 22 (6) ◽  
pp. 749-753
Author(s):  
V Spiehler ◽  
L Sun ◽  
D S Miyada ◽  
S G Sarandis ◽  
E R Walwick ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Enzyme Immunoassay ◽  
Correlation Coefficients ◽  
Gas Liquid Chromatography ◽  
Ultraviolet Spectrophotometry ◽  
Epileptic Patients ◽  
Quantitative Results ◽  
Analytical Procedures

Abstract Sera from epileptic patients were assayed for phenobarbital and diphenylhydantoin by four different analytical procedures. Quantitative results obtained by radioimmunoassay (I) and enzyme immunoassay (II) were compared to each other and to the results obtained on aliquots of the same sample by gas-liquid chromatography (III) and ultraviolet spectrophotometry (IV). For phenobarbital the correlation coefficients were I vs. II, 0.909; I vs. III, 0.947; II vs. III, 0.917; I vs. IV, 0.950; II vs. IV, 0.953. For diphenylhydantoin the correlation coefficients were I vs. II, 0.953; I vs. III, 0.951; II vs. III, 0.957; I vs. IV, 0.862; II vs. IV, 0.898. The immunoassays can be substituted for liquid chromatography or ultraviolet spectrophotometry without changing the resulting clinical interpretations.

Radioimmunoassay, enzyme immunoassay, spectrophotometry, and gas-liquid chromatography compared for determination of phenobarbital and diphenylhydantoin.

1976 ◽  
Vol 22 (6) ◽  
pp. 749-753 ◽  
Author(s):  
V Spiehler ◽  
L Sun ◽  
D S Miyada ◽  
S G Sarandis ◽  
E R Walwick ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Enzyme Immunoassay ◽  
Correlation Coefficients ◽  
Gas Liquid Chromatography ◽  
Ultraviolet Spectrophotometry ◽  
Epileptic Patients ◽  
Quantitative Results ◽  
Analytical Procedures

Abstract Sera from epileptic patients were assayed for phenobarbital and diphenylhydantoin by four different analytical procedures. Quantitative results obtained by radioimmunoassay (I) and enzyme immunoassay (II) were compared to each other and to the results obtained on aliquots of the same sample by gas-liquid chromatography (III) and ultraviolet spectrophotometry (IV). For phenobarbital the correlation coefficients were I vs. II, 0.909; I vs. III, 0.947; II vs. III, 0.917; I vs. IV, 0.950; II vs. IV, 0.953. For diphenylhydantoin the correlation coefficients were I vs. II, 0.953; I vs. III, 0.951; II vs. III, 0.957; I vs. IV, 0.862; II vs. IV, 0.898. The immunoassays can be substituted for liquid chromatography or ultraviolet spectrophotometry without changing the resulting clinical interpretations.

Quantitative Determination of Benzaldehyde in Flavors by Gas-Liquid Chromatography

1963 ◽  
Vol 46 (6) ◽  
pp. 950-951
Author(s):  
Richard L Brunelle ◽  
Glenn E Martin
Keyword(s):  
Liquid Chromatography ◽  
Liquid Phase ◽  
Benzoic Acid ◽  
Quantitative Determination ◽  
Solid Support ◽  
Specific Method ◽  
Gas Liquid Chromatography ◽  
Ultraviolet Spectrophotometry ◽  
Liquid Chromatography Separation

Abstract A rapid and specific method is given for determination of benzaldehyde in the presence of interfering substances by gas-liquid chromatography. Separation is effected on a ¼ column containing 2 5% Apiezon M as the liquid phase on a solid support of Chromoport XXX (60—80 mesh) at isothermal conditions, 100°C. The determination can be performed in the presence of benzoic acid and other interfering substances. Results are compared with those obtained by ultraviolet spectrophotometry on a variety of almond and cherry flavors.

Radioimmunoassay and enzyme immunoassay compared for determination of digoxin.

1976 ◽  
Vol 22 (12) ◽  
pp. 2029-2031 ◽  
Author(s):  
L Sun ◽  
V Spiehler
Keyword(s):  
Enzyme Immunoassay ◽  
Correlation Coefficients ◽  
Coefficients Of Variation ◽  
Quantitative Results ◽  
High Control

Abstract Patients' sera were analyzed for digoxin by using two different radioimmunoassays and an enzyme immunoassay. Quantitative results obtained by enzyme immunoassay (I) were compared to results obtained on aliquots of the same sample by the radioimmunoassays (II and III). The correlation coefficients were: I vs. II 0.90, n=108; I vs. III 0.94, n=102; and II vs. III 0.95, n=158. Day-to-day precision (10 days) on a low control (1.3 mug/liter) and a high control 3.0 mg/liter), expressed as coefficients of variation, were: I, 13% and 7.8%, II, 4.0% and 4.7%; and III, 8.9% and 4.2%. Ten digoxin-supplemented samples (0-8 mug/liter) were analyzed by the three methods. Correlation coefficients were: supplemented sample vs. I, O.99; supplemented sample vs. II, 0.97; supplemented sample vs. III, 0.98.

High-pressure liquid chromatography and enzyme immunoassay compared with gas chromatography for determing phenytoin.

1977 ◽  
Vol 23 (9) ◽  
pp. 1764-1766 ◽  
Author(s):  
R T Chamberlain ◽  
D T Stafford ◽  
A G Maijub ◽  
B C McNatt
Keyword(s):  
Gas Chromatography ◽  
High Pressure ◽  
Liquid Chromatography ◽  
Enzyme Immunoassay ◽  
Correlation Coefficients ◽  
Chromatographic Technique ◽  
Labor Costs ◽  
Gas Chromatographic Method ◽  
Liquid Chromatographic

Abstract We compared a gas-chromatographic method for determination of phenytoin with a high-pressure liquid chromatographic technique and with enzyme immunoassay by three instrumental procedures. More than 100 sera from patients being treated with this drug were assayed by all these techniques. The coefficient of variation was the lowest (4.0%) with liquid chromatography, but all methods gave a CV of less than 10%. The correlation coefficients for all methods exceeded 0.97 when compared to gas chromatography. Operation costs varied with the number of tests per batch, reagent costs, and operator labor costs. All assays gave comparable values for the therapeutic range, so it would be plausible to use more than one method in a situation where (e.g.) satellite laboratories handle different quantities of assays. In any of these techniques, interferences from carbamazepine, mephenytoin, phenobarbital, and primidone were negligible.

scholarly journals Comparative Study of the Anthranilic Acid Esters by Ultraviolet Spectrophotometry, Colorimetry, Gravimetry, and Gas-Liquid Chromatography

1965 ◽  
Vol 48 (2) ◽  
pp. 341-343
Author(s):  
Richard L Brunelle ◽  
Glenn E Martin ◽  
V G Ohanesian
Keyword(s):  
Liquid Chromatography ◽  
Chromatographic Method ◽  
Steam Distillation ◽  
Gas Liquid Chromatography ◽  
Ultraviolet Spectrophotometry ◽  
Characteristic Peak ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Acid Esters

Abstract A rapid and specific gas-liquid chromatographic method has been developed for the separation and quantitative determination of methyl and ethyl anthranilate in grape extracts and imitation grape flavors in the presence of interfering substances. Total methyl and ethyl anthranilate can be quantitatively determined in imitation grape flavors by ultraviolet spectrophotometry after steam distillation of the sample. Methyl and ethyl anthranilate show a characteristic peak at 335 mμ. Results for methyl and ethyl anthranilate by gas-liquid chromatography and ultraviolet spectrophotometry are compared with those obtained by the official AOAC methods.

Capillary gas chromatography of trimethyl- and methylethylbiphenyls

1980 ◽  
Vol 45 (11) ◽  
pp. 2919-2926 ◽  
Author(s):  
Jan Novrocík ◽  
Marta Novrocíková
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Stationary Phases ◽  
Correlation Coefficients ◽  
Gas Liquid Chromatography ◽  
Polar Medium ◽  
Alkyl Groups ◽  
Straight Line ◽  
Isomeric Methyl

Capillary gas-liquid chromatography with three columns impregnated with non-polar, medium and polar stationary phases has been used for determination of the Kováts indexes of methylethyl- and trimethylbiphenyls carrying the alkyl groups in the both rings. From elution data of isomeric methyl- and ethylbiphenyls the increments of methyl and ethyl groups have been determined, the theoretical Kováts indexes of the mentioned methylethyl- and trimethylbiphenyls have been calculated, and possibility of prediction and assessment of the Kováts indexes of these substances has been discussed. Parameters of the straight line given by the relation IApiez.L = k . Istat.phase(2) + q have been determined, and correlation coefficients of these relations have been estimated.

Semi-automated continuous-flow enzyme immunoassay for antiepileptic drugs in serum.

1983 ◽  
Vol 29 (10) ◽  
pp. 1790-1792 ◽  
Author(s):  
S Hayashi ◽  
S Kurooka ◽  
K Arisue ◽  
K Kohda ◽  
C Hayashi
Keyword(s):  
Liquid Chromatography ◽  
Enzyme Immunoassay ◽  
Antiepileptic Drugs ◽  
Continuous Flow ◽  
Cell Walls ◽  
Correlation Coefficients ◽  
Therapeutic Monitoring ◽  
Gas Liquid Chromatography ◽  
Serum Samples ◽  
Automated Method

Abstract We have developed a semi-automated method for measuring five kinds of antiepileptic drugs in serum by successfully adapting commercial competitive-binding enzyme immunoassay kits (MARKIT; Dainippon) for use with a continuous-flow analyzer (Technicon AutoAnalyzer II equipped with a dialyzer). The free enzyme-labeled drug is automatically separated by a microfilter from the competitive immunoreaction mixture between labeled and unlabeled drug for anti-drug immunoglobulin coupled to bacterial cell walls. The concentrations of the antiepileptic drugs in serum samples can be determined by automated measurement of enzyme activity of the enzyme-labeled drugs. Results of the semi-automated method correlated well with those obtained by manual enzyme immunoassay, gas-liquid chromatography, and "high-pressure" liquid chromatography. The correlation coefficients were all greater than 0.95, showing the practicality of this method for therapeutic monitoring of antiepileptic drugs.

Enzymatic Immunoassay vs. Gas/Liquid Chromatography for Determination of Phenobarbital and Diphenylhydantoin in Serum

1975 ◽  
Vol 21 (12) ◽  
pp. 1766-1768 ◽  
Author(s):  
Harold E Booker ◽  
Barbara A Darcey
Keyword(s):  
Liquid Chromatography ◽  
Enzyme Immunoassay ◽  
Antiepileptic Drugs ◽  
False Positives ◽  
Large Series ◽  
Gas Liquid Chromatography ◽  
False Negatives ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic

Abstract The antiepileptic drugs diphenylhydantoin and phenobarbital were measured in serum by a new commercially available enzyme immunoassay procedure ("EMIT," Syva Corp.). The procedure requires <5 min and no more than 50 µl of serum per determination. It is simple; only four steps (pipetting and diluting with an automatic pipettor-dilutor) are required before spectrophotometry. Twenty replicate analyses of a serum containing phenobarbital and diphenylhydantoin gave results with a CV of 6.8% and 9.1%, respectively. Results attained in a large series of patients were compared with results by a gas/liquid chromatographic procedure. For phenobarbital r = 0.97, and for diphenylhydantoin r = 0.98. No false negatives or false positives were encountered.

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