Enzyme-linked immunoassay for placental lactogen in human serum.

Abstract We describe an enzyme-linked immunoassay for measuring human placental lactogen (HPL) in serum. After suitable dilution, sera are pipetted into the wells of polyvinyl microtitre plates previously coated with anti-HPL. After incubation the sera are decanted, and replaced by a conjugate of horseradish peroxidase and anti-HPL. An amount of enzyme proportional to the concentration of HPL in the serum is bound to the wells via an antibody—HPL—antibody bridge. Peroxidase activity is measured by oxidation of o-phenylenediamine, the resulting color being related to the concentration of HPL. Results correlate well with those by radioimmunoassay (r = 0.96). Our between-assay coefficient of variation was 13%; no discernible effect of protein was observed. This 4-h assay can be used to monitor placental function in pregnancy.

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Enzyme-linked immunoassay for placental lactogen in human serum.

Clinical Chemistry ◽

10.1093/clinchem/26.6.745 ◽

1980 ◽

Vol 26 (6) ◽

pp. 745-749 ◽

Cited By ~ 11

Author(s):

D J MacDonald ◽

K M Nicol ◽

A Belfield ◽

M M Shah ◽

D S Mack

Keyword(s):

Horseradish Peroxidase ◽

Human Serum ◽

Peroxidase Activity ◽

Coefficient Of Variation ◽

Placental Lactogen ◽

Human Placental Lactogen ◽

Placental Function ◽

Discernible Effect ◽

In Pregnancy

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Human Placental Lactogen as an Index of Placental Function: Plasma Measurements in Normal and Pathological Pregnancies

Therapy of Feto-Placental Insufficiency ◽

10.1007/978-3-642-66008-5_6 ◽

1975 ◽

pp. 30-34

Author(s):

G. Culucci ◽

G. C. Englaro ◽

G. C. Tosolini ◽

F. Di Chiara ◽

E. Desole ◽

...

Keyword(s):

Placental Lactogen ◽

Human Placental Lactogen ◽

Placental Function ◽

Plasma Measurements

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Automated radioimmunoassay of choriomammotropin (human placental lactogen).

Clinical Chemistry ◽

10.1093/clinchem/22.10.1655 ◽

1976 ◽

Vol 22 (10) ◽

pp. 1655-1658 ◽

Cited By ~ 5

Author(s):

A Castro ◽

I Prieto ◽

C Wunsch ◽

G Ertingshausen ◽

H Malkus

Keyword(s):

Coefficient Of Variation ◽

Placental Lactogen ◽

Clinical Samples ◽

Single System ◽

Human Placental Lactogen

Abstract We describe a totally automated procedure for radioimmunoassay of choriomammotropin, in which all phases of the assay are automated in a single system ("Centria"). This system permits the simultaneous incubation and separation of many samples in a nonequilibrium assay, and measurements are obtained in less than 30 min. Results for clinical samples by reference radioimmunossay methodology and with the Centria system compared uniformly well: y=0.91x - 0.87;r=0.94. The coefficient of variation for samples run in duplicate on the same day was 5.2%, 7.4% for samples run on different days. The specificity, sensitivity, simplicity, and speed of this system makes it a useful new tool for kinetic, nonequilibrium immunoassay.

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8A 1. Hormone levels in pregnancy—I 274. Parallel assay of estradiol, estriol and human placental lactogen in normal pregnancy

Journal of Steroid Biochemistry ◽

10.1016/0022-4731(74)90419-1 ◽

1974 ◽

Vol 5 (4) ◽

pp. 361

Author(s):

P.G. Crosignani ◽

L. Trojsi ◽

G.C. Lombroso ◽

A. Attanasio ◽

R. Malvano

Keyword(s):

Normal Pregnancy ◽

Placental Lactogen ◽

Human Placental Lactogen ◽

Hormone Levels ◽

In Pregnancy

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Plasma GHRH, CRH, ACTH, β-endorphin, human placental lactogen, GH and cortisol concentrations at the third trimester of pregnancy

Acta Endocrinologica ◽

10.1530/acta.0.1200785 ◽

1989 ◽

Vol 120 (6) ◽

pp. 785-789 ◽

Cited By ~ 15

Author(s):

W. Jeske ◽

P. Soszyński ◽

W. Rogoziński ◽

E. Lukaszewicz ◽

W. Latoszewska ◽

...

Keyword(s):

Gestational Age ◽

Plasma Concentrations ◽

Maternal Plasma ◽

Placental Lactogen ◽

Strongly Correlated ◽

Human Placental Lactogen ◽

Strong Correlations ◽

Third Trimester ◽

Placental Function ◽

Duration Of Pregnancy

Abstract. The aim of the study was to determine the concentration of GHRH and CRH in maternal plasma during the 3rd trimester of pregnancy and to search for the possible correlations with related hormones such as ACTH, β-endorphin, cortisol, GH and human placental lactogen. Patients consisted of 31 healthy pregnant women (20–39 years) divided according to duration of pregnancy into 2 groups: I. from 26 to 32 pregnancy week N = 13), II. from 33 to 39 week (N = 18), and of 7 women evaluated 3 days after delivery. All listed hormones except ACTH were measured by RIA (GHRH, CRH and β-endorphin-like immunoreactivity after extraction with silic acid) and ACTH by IRMA. In the late 3rd trimester plasma levels of CRH (P< 0.001), ACTH (P< 0.02), β-endorphin (P< 0.05), cortisol (P< 0.025), as well as GHRH (P< 0.002) and human placental lactogen (hPL) (P< 0.001) were increased in comparison to early 3rd trimester, whereas 3 days after delivery CRH and GHRH became undetectable and those of ACTH and cortisol decreased significantly. The CRH plasma concentrations were found to be strongly correlated with gestational age (r = 0.86, P< 0.001) but not with ACTH and cortisol. GHRH levels correlated mainly with human placental lactogen concentrations (r = 0.64, P< 0.001). Conclusion: In maternal plasma at the 3rd trimester of pregnancy, apart from the known markedly elevated CRH, the GHRH level was also raised. Strong correlations between CRH and gestational age and those between GHRH and human placental lactogen suggest that there is a relationship between these neurohormones and the placental function.

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Obesity and regulation of human placental lactogen production in pregnancy

Journal of Neuroendocrinology ◽

10.1111/jne.12859 ◽

2020 ◽

Vol 32 (11) ◽

Author(s):

Peter A. Cattini ◽

Yan Jin ◽

Jessica S. Jarmasz ◽

Noshin Noorjahan ◽

Margaret E. Bock

Keyword(s):

Placental Lactogen ◽

Human Placental Lactogen ◽

In Pregnancy

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Enzyme-linked immunosorbent choriomammotropin assay.

Clinical Chemistry ◽

10.1093/clinchem/25.2.227 ◽

1979 ◽

Vol 25 (2) ◽

pp. 227-229 ◽

Cited By ~ 4

Author(s):

D Dittmar ◽

N Monji ◽

A Cid ◽

H Malkus ◽

A Castro

Keyword(s):

Immunosorbent Assay ◽

Coefficient Of Variation ◽

Regression Equation ◽

Placental Lactogen ◽

Clinical Samples ◽

Enzyme Linked Immunosorbent Assay ◽

Good Precision ◽

Human Placental Lactogen ◽

Mean Concentration ◽

Beta Galactosidase

Abstract We have devloped an enzyme-linked immunosorbent assay for determining choriomammotropin (human placental lactogen) in serum. Unlabeled hormone competes with choriomammotropin-beta-galactosidase conjugate for antibody bound to polystyrene tubes. The entire assay can be performed in 2.5 h with good precision. The coefficient of variation for one sample with a mean concentration of 5.6 mg/L, assayed 10 times on the same day, was 5.7%. The coefficient of variation for nine samples (3.5 to 9.0 mg/L) assayed on five different days was 7.9%. Forty-eight clinical samples were assayed (y) and compared with results obtained by radial immunodiffusion (x). The resulting regression equation was: y = 1.05x + 0.78; r = 0.91.

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