Determination of urinary oxalate with commercially available oxalate oxidase.

1983 ◽  
Vol 29 (4) ◽  
pp. 700-702 ◽  
Author(s):  
J E Buttery ◽  
N Ludvigsen ◽  
E A Braiotta ◽  
P R Pannall
Keyword(s):  
Chemical Method ◽  
Oxalate Oxidase ◽  
Regression Equation ◽  
Urinary Oxalate ◽  
Enzymatic Method ◽  
Analytical Recovery ◽  
Wide Range ◽  
Better Than

Abstract For this direct colorimetry of urinary oxalate, commercially available oxalate oxidase (EC 1.2.3.4) is used. The urine is first diluted, to diminish the effect of interfering substances. Analytical recovery of oxalate from urines with five different oxalate concentrations (0.4 to 2.0 mmol/L) ranged from 92 to 109% (mean 99%). The within-day and between-day precision (CV) of the method for a wide range of oxalate concentrations averaged better than 10%. There is good correlation (r = 0.977) between this enzymatic method (y) and the chemical method of Hodgkinson and Williams (x) [Clin Chim Acta 36: 127-132, 1972], the regression equation being y = 1.014x + 0.061. Urines with added ascorbate give falsely increased results. The proposed method is inexpensive and simple to perform.

scholarly journals Determination of Urinary Oxalate with Arylamine Glass-Bound Sorghum Oxalate Oxidase and Horseradish Peroxidase

2016 ◽  
Vol 4 (3) ◽  
pp. 346-351 ◽  
Author(s):  
M. Thakur ◽  
A.K. Bhargava ◽  
C.S. Pundir
Keyword(s):  
Horseradish Peroxidase ◽  
Urinary Stone ◽  
Oxalate Oxidase ◽  
Urinary Oxalate ◽  
Colour Reaction ◽  
Stone Formers ◽  
Analytical Recovery ◽  
Method R ◽  
Apparently Healthy

We describe an enzymic colourimetric method for determination of oxalate level in urine using arylamine glass-bound sorghum leaf oxalate oxidase and horseradish peroxidase. The method is based on quantification of H2O2 generated from oxidation of urinary oxalate by immobilized oxalate oxidase, by a colour reaction consisting of 4-aminophnazone, phenol and immobilized peroxidase as chromogen. Minimum detection limit of the method was 0.05 mmol/l. Analytical recovery of added oxalate in urine was 96.8± 3.0% (mean ±S.D.). Within and between day coefficient of variation (CV) for urinary oxalate in urine were < 3.5% and <6.46 % respectively. The urinary oxalate values in apparently healthy and urinary stone formers as measured by the present method were correlated with those by modified Sigma Kit method (r= 0.929). The method has the advantages that it provides ca 200 times reuse of oxalate oxidase and peroxidase and free from interferences by Cl- and NO3- normally found in urine. Int J Appl Sci Biotechnol, Vol 4(3): 346-351

Kinetic Enzymatic Method for Determining Serum Creatinine

1975 ◽  
Vol 21 (10) ◽  
pp. 1422-1426 ◽  
Author(s):  
Gerald A Moss ◽  
Richard J L Bondar ◽  
Diane M Buzzelli
Keyword(s):  
Serum Creatinine ◽  
Accurate Determination ◽  
Test Subject ◽  
Rate Of Change ◽  
Enzymatic Method ◽  
Inhibitory Effects ◽  
Standard Curve ◽  
Analytical Recovery ◽  
Enzymatic Procedure

Abstract Creatinine amidohydrolase is used to measure serum creatinine in a totally enzymatic procedure. Creatine, produced by hydrolysis, is acted upon by creatine kinase, and then by pyruvate kinase and lactate dehydrogenase, to result in a change in absorbance at 340 nm. The amount of creatinine present is related to the rate of change in A340 and is determined from a standard curve. Absorbance and concentration are linearly related to 100 mg/liter and only 250 µl of serum is required. At 1.0 g/liter, heparin, oxalate, citrate, ethylenediaminetetraacetate, ascorbate, or glucose had no significant effect on the accurate determination of creatinine; higher concentrations (30 g/liter) had inhibitory effects on the test. Analytical recovery of creatinine added to either normal or abnormal sera averaged 102%. When results of this procedure and of the standard direct Jaffé test were compared, the latter were significantly higher. Unlike the Jaffé method, the present method of determining creatinine is rapid (about 10 min per test), subject to few or no interfering substances, and requires no serum deproteinization.

scholarly journals Use of a Ruthenium(III), Iron(II), and Nickel(II) Hexacyanometallate-Modified Graphite Electrode with Immobilized Oxalate Oxidase for the Determination of Urinary Oxalate

2001 ◽  
Vol 84 (6) ◽  
pp. 1927-1933 ◽  
Author(s):  
Stjepan Milardović ◽  
Zorana Grabarić ◽  
Vlatko Rumenjak ◽  
Nenad Blau ◽  
Dražen Milošević
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Graphite Electrode ◽  
Oxalate Oxidase ◽  
Urine Samples ◽  
Daily Routine ◽  
Urinary Oxalate ◽  
Catalyzed Oxidation ◽  
Linear Concentration Range ◽  
Linear Concentration

Abstract This paper describes the performance of a biosensor with an Ru(III), Ni(II), and Fe(II) hexacyanometallate-modified graphite electrode and immobilized oxalate oxidase for the determination of urinary oxalate. The addition of ruthenium enhances the electrochemical reversibility and chemical stability of the electrocrystallized layer and improves the sensitivity of the biosensor. Hydrogen peroxide, produced by the enzyme-catalyzed oxidation of oxalate, was measured at −50 mV vs an Hg|Hg2Cl2|3M KCl electrode in a solution of pH 3.6 succinic buffer, 0.1M KCl, and 5.4mM ethylenediaminetetraacetic acid. The linear concentration range for the determination of oxalate was 0.18–280 μM. The recoveries of added oxalate (10–35 μM) from aqueous solution ranged from 99.5 to 101.7%, whereas from urine samples without oxalate (or with a concentration of oxalate below the detection limit) the recoveries of added oxalate ranged from 91.4 to 106.6%. The oxalate in 24 h urine samples, taken during their daily routine from 35 infants and children, was measured and found to range from 0.6 to 121.7 mg/L. There were no interferences from uric acid, acetylsalicylic acid, and urea in the concentration range investigated, but paracetamol and ascorbic acid did interfere. A good correlation (R2 = 0.9242) was found between values obtained for oxalate in real urine samples by 2 laboratories, with the proposed biosensor and ion chromatography, respectively.

Rapid enzymatic determination of urinary oxalate.

1989 ◽  
Vol 35 (12) ◽  
pp. 2330-2333 ◽  
Author(s):  
M G Li ◽  
M M Madappally
Keyword(s):  
Enzyme Assay ◽  
Divalent Cations ◽  
Urinary Oxalate ◽  
Assay Sensitivity ◽  
Color Development ◽  
Assay Procedure ◽  
Enzymatic Determination ◽  
Analytical Recovery ◽  
Urine Specimens

Abstract This new reagent kit for the quantitative measurement of oxalate in urine is a modification of an earlier Sigma oxalate assay procedure (procedure no. 590), a coupled enzyme assay involving oxalate oxidase and horseradish peroxidase. The new analytical procedure includes methods for processing urine specimens to eliminate interference with oxalate color development at 590 nm by ascorbic acid, divalent cations, and other urinary constituents. The reaction is complete in less than 5 min, and results are linearly related to oxalate concentration up to at least 1 mmol/L. Assay sensitivity and within-run and between-run precision were within the limits acceptable for other urinary oxalate procedures. Analytical recovery of added oxalate was close to 100%. This specific, simple, rapid procedure is suitable for routine clinical use.

HemoCue: Evaluation of a Portable Photometric System for Determining Glucose in Whole Blood

1992 ◽  
Vol 38 (8) ◽  
pp. 1479-1482 ◽  
Author(s):  
L Ashworth ◽  
I Gibb ◽  
K G Alberti
Keyword(s):  
Whole Blood ◽  
Glucose Dehydrogenase ◽  
Glucose Measurement ◽  
Photometric System ◽  
Analytical Recovery ◽  
Wide Range ◽  
Regression Slopes ◽  
High Concentrations ◽  
Better Than ◽  
Positive Interference

Abstract We assessed the HemoCue system for measuring glucose in 5 microL of whole blood. A glucose dehydrogenase-based reaction is used with dried reagents contained in disposable microcuvettes, which are filled with blood by capillary action. Automated hexokinase and YSI 23AM glucose analyzer methods were used for comparison. Overall imprecision (CV) was better than 4.5%, with no significant differences in results between three different HemoCue photometers and four batches of microcuvettes. Regression slopes (+/- SE) were 0.947 (0.011) with the YSI and 0.966 (0.015) with the hexokinase method. Analytical recovery of added glucose was 101-106%, and the system functioned with hematocrits up to 0.65. Bilirubin up to 453 mumol/L did not interfere, but high concentrations of endogenous (greater than 3 mmol/L) and exogenous triglycerides gave positive interference. The system proved stable and robust under a wide range of storage and handling conditions; performance was impaired only at high ambient temperature (37 degrees C). We conclude that the HemoCue system should prove useful for glucose measurement; further testing outside the laboratory is warranted.

Modifications to commercial oxalate oxidase based determination of urinary oxalate: A method suitable for routine clinical analysis

1990 ◽  
Vol 23 (2) ◽  
pp. 173-177 ◽  
Author(s):  
Angelo Mazzuchin ◽  
Lisa Michelutti ◽  
Hermann Falter
Keyword(s):  
Clinical Analysis ◽  
Oxalate Oxidase ◽  
Urinary Oxalate

Quantification of urinary oxalate with oxalate oxidase from beet stems.

1983 ◽  
Vol 29 (10) ◽  
pp. 1815-1819 ◽  
Author(s):  
D M Obzansky ◽  
K E Richardson
Keyword(s):  
Horseradish Peroxidase ◽  
Isotope Dilution ◽  
Reference Interval ◽  
Oxalate Oxidase ◽  
Exchange Chromatography ◽  
Urinary Oxalate ◽  
Cation Exchange Chromatography ◽  
Standard Curve ◽  
The Difference

Abstract We describe an automated (ABA-100) enzymic method for determination of urinary oxalate by use of oxalate oxidase (EC 1.2.3.4) isolated from beet stems. The H2O2 produced by the oxidation of oxalate by oxalate oxidase is measured by coupling with oxidation and conjugation of 3-methyl-3-benzothiazolinone hydrazone with N,N-dimethylaniline with catalysis by horseradish peroxidase. The resulting indamine dye is measured spectrophotometrically by the difference in absorption at 500 and 600 nm. Interfering substances are removed by oxidation with acidic ferric chloride and by cation-exchange chromatography. The assay is sensitive to 5 mg of urinary oxalate per liter, the standard curve is linear to 70 mg/L, and the procedure requires less than 3 h for completion. The within-run CV was less than 1.6%, the between-day CV less than 5.6%. The oxalate oxidase method results in a mean and reference interval for oxalate excretion that are comparable with those by isotope dilution, gas-chromatographic, colorimetric, and other enzymic procedures.

Evaluation and comparison of a radiative energy attenuation method for determining cholesterol in serum.

1985 ◽  
Vol 31 (4) ◽  
pp. 605-608 ◽  
Author(s):  
P L Cary ◽  
C A Johnson ◽  
P D Whitter ◽  
J W Parker
Keyword(s):  
Regression Analysis ◽  
Linear Regression ◽  
High Performance ◽  
Linear Regression Analysis ◽  
Radiative Energy ◽  
Comparison Methods ◽  
Analytical Recovery ◽  
Wide Range

Abstract Determination of cholesterol by a radiative energy attenuation (REA) technique was evaluated and compared with results obtained by the Boehringer Mannheim High Performance Cholesterol Assay and the Du Pont aca. Within-assay and between-assay CVs for the REA method, for two sets of controls, were both less than 5%. We observed no interference with lipemic samples. Analytical recovery averaged 102.8%. We used all three methods for parallel determinations of 217 patients' samples containing a wide range of cholesterol concentrations. Linear regression analysis of the REA results vs those of the comparison methods were as follows: REA = 1.03 Boehringer - 0.072 (r = 0.993) and REA = 1.02 aca - 0.048 (r = 0.995). We also discuss bilirubin interference with the REA method for cholesterol.

Oxalate oxidase from banana peel for determination of urinary oxalate.

1985 ◽  
Vol 31 (4) ◽  
pp. 649-649 ◽  
Author(s):  
K G Raghavan ◽  
T P Devasagayam
Keyword(s):  
Oxalate Oxidase ◽  
Banana Peel ◽  
Urinary Oxalate

A simplified and rapid enzymatic method for determination of urinary oxalate

1974 ◽  
Vol 55 (1) ◽  
pp. 29-39 ◽  
Author(s):  
P.C. Hallson ◽  
G.Alan Rose
Keyword(s):  
Urinary Oxalate ◽  
Enzymatic Method
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