Effect of storage on measurement of ionized calcium in serum of uremic patients.

1985 ◽  
Vol 31 (2) ◽  
pp. 287-289 ◽  
Author(s):  
N I Nikolakakis ◽  
A M De Francisco ◽  
R S Rodger ◽  
E Gaiger ◽  
T H Goodship ◽  
...  
Keyword(s):  
Whole Blood ◽  
Room Temperature ◽  
Ionized Calcium ◽  
Measured Concentration ◽  
Analytical Variance ◽  
Serum Ionized Calcium ◽  
Uremic Patients ◽  
Serum Storage

Abstract We studied, in 70 acidotic and non-acidotic uremic patients, the analytical variance in serum ionized calcium as related to duration and temperature of storage. Storage of serum or whole blood at 4 degrees C for as long as 6 h did not significantly alter the measured concentration of ionized calcium in the serum. Storage at room temperature for 6 h, or longer at 4 degrees C or -20 degrees C, resulted in inaccuracies in 39 to 79% of the samples of serum and in 38 to 92% of the samples of whole blood. These errors were not negated by correcting the values for ionized calcium to a pH of 7.40. Indeed, corrected values for calcium were even more unreliable in acidotic patients. We conclude that samples from uremic patients should be analyzed for ionized calcium within 2 h, or within 6 h if stored at 4 degrees C.

Measurement of Serum Ionized Calcium with the Ion-Exchange Electrode

1972 ◽  
Vol 18 (12) ◽  
pp. 1459-1462 ◽  
Author(s):  
V L Subryan ◽  
M M Popovtzer ◽  
S D Parks ◽  
E B Reeve
Keyword(s):  
Ion Exchange ◽  
Reproducibility Of Results ◽  
Room Temperature ◽  
Calcium Concentration ◽  
Ionized Calcium ◽  
Control Solution ◽  
Electrode Response ◽  
Serum Ionized Calcium ◽  
Evacuated Tubes

Abstract We found no differences in pH or ionized calcium concentration of serum from blood either collected into evacuated tubes or collected with syringes and separated under oil. Sera can be stored frozen in 1-ml plastic insulin-type syringes for one to three days without significant changes in pH or ionized calcium concentration, whereas storage under oil with or without re-equilibration with CO2 is associated with significant changes in both. Standards prepared with trypsin and triethanolamine give faster electrode response, and their ionized calcium concentrations remain unchanged for 6 h at room temperature. We suggest a not entirely satisfactory control solution of buffered protein, for use in assessing reproducibility of results.

scholarly journals Whole blood versus serum ionized calcium concentrations in dialysis patients

2014 ◽  
Vol 29 (2) ◽  
pp. 226 ◽  
Author(s):  
Seok Hui Kang ◽  
Kyu Hyang Cho ◽  
Jong Won Park ◽  
Kyung Woo Yoon ◽  
Jun Young Do
Keyword(s):  
Whole Blood ◽  
Ionized Calcium ◽  
Dialysis Patients ◽  
Serum Ionized Calcium

Plasma Thromboplastin Component (Christmas Factor, Factor IX) Levels in Stored Human Blood and Plasma

1960 ◽  
Vol 04 (03) ◽  
pp. 376-388 ◽  
Author(s):  
J Dieter Geratz ◽  
John B. Graham
Keyword(s):  
Human Plasma ◽  
Human Blood ◽  
Whole Blood ◽  
Room Temperature ◽  
Close Agreement ◽  
Factor Ix ◽  
Deficient Patient ◽  
Glass Tubes ◽  
Disodium Hydrogen Citrate ◽  
Bank Blood

Summary1. PTC activity was assayed in 26 units of human plasma prepared from whole blood stored for 3 weeks at 4° C. The plasma had been frozen and stored at — 20° C for additional periods ranging from a few days to 4 months. High PTC activity was still present in the plasma at the end of this period, the activity averaging 95% of normal.2. The PTC activity of 19 samples of “reclaimed“ plasma stored for an additional 6 months at — 20° C decreased by an average of 23%. This decrease was statistically significant.3. Liquid plasma kept at room temperature for 5½—7½ months contained no PTC activity.4. Lyophilized plasma stored at room temperature for 6—8 years contained an average of 30% PTC activity. Lyophilized plasma stored at — 20° C for 4 years contained 68% PTC activity.5. ACD and disodium hydrogen citrate anticoagulant solutions served equally well in preserving PTC activity in whole blood stored in glass tubes over a period of 3 weeks at 4° C.6. “Reclaimed“ plasma from outdated bank blood provided effective hemostasis in two operations for the removal of 20 teeth from a severely PTC-deficient patient.7. The high PTC activity of “reclaimed“ plasma was confirmed by the close agreement between the PTC level expected in a PTC deficient patient after transfusion of such plasma and that observed.

scholarly journals In vitro characteristics and in vivo platelet quality of whole blood treated with riboflavin and UVA / UVB light and stored for 24 hours at room temperature

Transfusion ◽  
2021 ◽  
Vol 61 (S1) ◽  
Author(s):  
Turid Helen Felli Lunde ◽  
Lindsay Hartson ◽  
Shawn Lawrence Bailey ◽  
Tor Audun Hervig
Keyword(s):  
Whole Blood ◽  
Room Temperature

INFLUENCE OF SAMPLING METHOD ON WHOLE BLOOD IONIZED CALCIUM VALUES

1989 ◽  
Vol 71 (Supplement) ◽  
pp. A418 ◽  
Author(s):  
I. CONSTANT ◽  
B. BENETEAU ◽  
B. JUST ◽  
E. DELVA ◽  
M. VAUBOURDOLLE ◽  
...  
Keyword(s):  
Whole Blood ◽  
Sampling Method ◽  
Ionized Calcium

Serial blood cultures from canine stored whole blood held at room temperature for 24 h

2009 ◽  
Vol 18 (3) ◽  
pp. 279-281 ◽  
Author(s):  
J. S. Beymer ◽  
E. Rudloff ◽  
R. Kirby ◽  
T. J. Novicki ◽  
F. M. Moore
Keyword(s):  
Whole Blood ◽  
Room Temperature ◽  
Blood Cultures ◽  
Serial Blood

SERUM IONIZED CALCIUM IN NEWBORN INFANTS

PEDIATRICS ◽  
1972 ◽  
Vol 49 (6) ◽  
pp. 841-846
Author(s):  
David M. Brown ◽  
James Boen ◽  
Audrey Bernstein
Keyword(s):  
Biochemical Parameters ◽  
Newborn Infants ◽  
Ionized Calcium ◽  
Entire Group ◽  
Total Calcium ◽  
Serum Total Protein ◽  
Ionic Calcium ◽  
Serum Ionized Calcium ◽  
Absolute Concentrations ◽  
Positive Correlations

Relationships of serum ionized calcium with several biochemical parameters have been examined in newborn infants under 72 hours of age. Hypocalcemia was defined as plasma total calcium below 7.5 mg/100 ml. There was a linear correlation between plasma total calcium and ionized calcium in the entire group. Other positive correlations included: (1) plasma total calcium and serum total protein, albumin, and globulins and (2) absolute concentrations of ionic calcium and albumin. No statistically significant correlations were observed between either total or ionized calcium and serum phosphorus, percent ionized calcium, capillary pH, or Pco2. The derived values for serum ionic calcium from the McLean-Hastings nomogram did not correlate with the plasma ionic calcium as determined by the ion-specific electrode and may not be used in newborn infants to predict accurately the level of plasma ionic calcium.

Sign in / Sign up

Export Citation Format

Share Document