Variations in Levels of Transaminases and Lactic Dehydrogenase in Bank Blood

1962 ◽  
Vol 8 (6) ◽  
pp. 626-629 ◽  
Author(s):  
Leonard V Crowley
Keyword(s):  
Blood Donors ◽  
Lactic Dehydrogenase ◽  
Screening Procedure ◽  
Dextrose Solution ◽  
Acid Citrate Dextrose ◽  
Citrate Dextrose ◽  
Bank Blood

Abstract Results of the assay of transaminases and lactic dehydrogenase in blood, proposed as a screening procedure for subclinical hepatitis in blood donors, show that the method of sampling influences the analytic results. Determinations of GOT and GPT may prove useful if made on specimens separated from cells soon after collection. Plasma in contact with cells from blood preserved in acid citrate dextrose solution appears to yield good results up to 21 days. Lactic-dehydrogenase levels are of limited value for this screening.

scholarly journals Reversal of granulocyte adherence to nylon fibers using local anesthetic agents: possible application to filtration leukapheresis

Blood ◽  
1977 ◽  
Vol 50 (2) ◽  
pp. 213-225 ◽  
Author(s):  
CA Schiffer ◽  
FT Sanel ◽  
VB Young ◽  
J Aisner
Keyword(s):  
Local Anesthetic ◽  
Membrane Integrity ◽  
Lactic Dehydrogenase ◽  
Anesthetic Agents ◽  
Cytoplasmic Vacuolization ◽  
Bactericidal Capacity ◽  
Ultrastructural Appearance ◽  
Acid Citrate Dextrose ◽  
Citrate Dextrose ◽  
Granulocyte Function

Abstract The effects of the cationic anesthetic agents tetracaine and lidocaine on granulocyte function, morphology, and adherence to nylon fibers were studied in an attempt to improve current methods of granulocyte collection by filtration leukapheresis (FL). When dissolved in acid- citrate-dextrose (ACD) plasma, these drugs significantly increased granulocyte elution from the fibers in a dose-related fashion. Granulocytes exposed to tetracaine and lidocaine remained more than 95% viable, retained normal bactericidal capacity after the drugs were washed from the cells, and had preserved membrane integrity, as evidenced by the normal ultrastructural appearance of tetracaine- exposed cells and an absence of leakage of lysozyme or lactic dehydrogenase. Granulocytes eluted with the anesthetic agents were rounded in shape with a reduction in the number of filopodial cytoplasmic projections and a relative absence of cytoplasmic vacuolization when compared to granulocytes eluted with ACD plasma alone. Dose-related inhibition of phagocytosis and adherence, which was largely reversible after washing the granulocytes, was noted. Greater than 95% of the lidocaine could be removed from the eluate with a single centrifugation and resuspension, indicating that granulocytes prepared by FL with anesthetic-enhanced elution could be potentially transfusable.

scholarly journals Satisfactory Red Cell Viability with Slight Excess of Acid Citrate Dextrose

Blood ◽  
1968 ◽  
Vol 32 (3) ◽  
pp. 469-472 ◽  
Author(s):  
KLAUS MAYER ◽  
JOSEPH D'AMARO
Keyword(s):  
Cell Viability ◽  
Cell Survival ◽  
Blood Donors ◽  
Red Cells ◽  
Red Cell ◽  
Slight Excess ◽  
Great Excess ◽  
Red Cell Survival ◽  
Acid Citrate Dextrose ◽  
Citrate Dextrose

Abstract Platelet suspensions obtained from blood donors are improved by increased acidification. The simplest way to accomplish this is to collect less blood into the standard quantity of ACD. Since we have previously reported an impairment in red cell viability when blood is collected in a great excess of ACD, it became pertinent to test the survival of stored red cells collected in a "slight" excess of ACD. The volume of the blood collected was lowered to 375 ml. in 75 ml. ACD (N. I. H. formula A). At this ratio the pH. was 6.5 which is sufficiently low to minimize platelet clumping. The red cells were separated, stored for 21 days at 4 C., and viability was tested by the 51Chromate method. The results showed adequate red cell survival for blood collected and stored in this manner.

Implications of anticoagulants and gender on cell counts and growth factor concentration in platelet-rich plasma and platelet-rich gel supernatants from rabbits

2016 ◽  
Vol 29 (02) ◽  
pp. 115-124 ◽  
Author(s):  
Juan González ◽  
Catalina López ◽  
Jorge Carmona
Keyword(s):  
Growth Factor ◽  
Whole Blood ◽  
Sodium Citrate ◽  
Platelet Rich Plasma ◽  
Dextrose Solution ◽  
Cell Counts ◽  
Acid Citrate Dextrose ◽  
And Gender ◽  
Citrate Dextrose ◽  
Factor Concentration

SummaryObjectives: Our objectives were as follows: 1) to validate a protocol for producing rabbit platelet-rich plasma (PRP); 2) to determine the influence of two anticoagulants, sodium citrate and acid-citrate-dextrose solution A, and gender on cell count in PRP and growth factor concentration in pure platelet-rich gel supernatants; 3) to correlate the variables evaluated.Methods: Whole blood from 18 New Zealand rabbits (9 males and 9 females) was obtained with sodium citrate and acid- citrate-dextrose solution A for processing PRP fractions (A and B), which were evaluated for haematology. The PRP fractions were either activated with calcium gluconate or lysated with a detergent. The concentrations of transforming growth factor beta 1 and platelet-derived growth factor BB were assayed by ELISA.Results: The sodium citrate PRP-B had significantly higher counts of platelets in comparison to PRP-A and whole blood obtained with the same anticoagulant and the homologous acid-citrate-dextrose solution A PRP fraction. The sodium citrate PRP-A had a significantly higher count of leukocytes compared to the homologous acid-citrate-dextrose solution A fraction. All the PRP fractions had a significant leuko-reduction when compared to whole blood. The sodium citrate PRP-A fraction from female rabbits had significantly lower platelet counts and significantly higher leukocyte counts than the same acid-citrate-dextrose solution A fraction. Growth factor concentration was not affected by the type of anticoagulant or gender.Clinical significance: The type of anticoagulant and gender affected the cell counts in PRP, but they did not influence the growth factor concentration. More complete rabbit PRP studies should be performed before evaluating this type of substance in models of disease.

scholarly journals AN ACID CITRATE-DEXTROSE SOLUTION WITH LOW WATER VOLUME AND LOW DEXTROSE CONCENTRATION 1

1947 ◽  
Vol 26 (4) ◽  
pp. 678-686 ◽  
Author(s):  
Max M. Strumia ◽  
Alton D. Blake ◽  
John J. McGraw ◽  
Miss Margaret Dolan ◽  
Miss Louise Colwell
Keyword(s):  
Water Volume ◽  
Dextrose Solution ◽  
Acid Citrate Dextrose ◽  
Citrate Dextrose

scholarly journals Effects of Anticoagulants and Storage on Granulocyte Function in Bank Blood

Blood ◽  
1974 ◽  
Vol 43 (2) ◽  
pp. 207-217 ◽  
Author(s):  
Jeffrey McCullough ◽  
Sandra J. Carter ◽  
Paul G. Quie
Keyword(s):  
Whole Blood ◽  
Normal Function ◽  
Bactericidal Assay ◽  
Acid Citrate Dextrose ◽  
Citrate Dextrose ◽  
Granulocyte Function ◽  
And Storage ◽  
Citrate Phosphate ◽  
Bank Blood

Abstract Storage of granulocytes for transfusion has not been practical because it has been considered that granulocyte function in bank blood is retained for only a few hours after collection. In the present study, granulocyte function was evaluated using the bactericidal assay and the quantitative nitroblue tetrazolium (NBT) method. Granulocytes from whole blood collected into acid citrate dextrose (ACD), citrate phosphate dextrose (CPD), heparin, ion exchange, and sodium citrate anticoagulants showed no functional impairment after 24 hr of storage at 4°C. With further storage, all granulocytes showed a loss of NBT activity. However, after 48 and 72 hr, granulocytes from whole blood stored in ACD and CPD killed the expected number of bacteria in the bactericidal assay. Thus, when tested in vitro, granulocytes maintain normal function, at least during the first 24 hr after collection when stored in certain anticoagulants under standard blood bank conditions.

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