The development of microarray technology offers the unprecedented possibility of studying the expression of thousands of genes in one experiment. Its exploitation in the glycobiology field will eventually allow the parallel investigation of the expression of many glycosyltransferases, which will ultimately lead to an understanding of the regulation of glycoconjugate synthesis. While numerous gene arrays are available on the market, e.g. the Affymetrix GeneChip® arrays, glycosyltransferases are not adequately represented, which makes comprehensive surveys of their gene expression difficult. This chapter describes the main issues related to the establishment of a custom glycogenes array.
P063 Representative and comprehensive analysis of colonic and ileac biopsies from IBD patients by gene expression profiling using the straightforward, fast, and affordable novel application Whole Transcriptome AmpliSeq on the Ion Torrent NGS platform
