P081 Effect of bile acid on lymphocyte migration in the small intestine

The transport of taurocholic and glycocholic acids by the small intestine of rats and guinea pigs against a concentration gradient was studied by the everted gutsac technique. Transport of these substances is limited to the distal ileal segment. This transport is inhibited by anoxia, dinitrophenol and sodium azide. The system has a transport maximum. On the basis of these criteria bile acid reabsorption is considered to occur by active transport.

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Differential intestinal deconjugation of taurine and glycine bile acid N-acyl amidates in rats

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1992.262.2.g351 ◽

1992 ◽

Vol 262 (2) ◽

pp. G351-G358

Author(s):

R. Zhang ◽

S. Barnes ◽

R. B. Diasio

Keyword(s):

High Performance Liquid Chromatography ◽

Small Intestine ◽

Liquid Chromatography ◽

Bile Acid ◽

Chenodeoxycholic Acid ◽

High Performance ◽

Biliary Fistula ◽

The Difference ◽

The Stability ◽

Rat Bile

Mechanisms responsible for the difference in the relative amounts of taurine- and glycine-conjugated bile acid N-acyl amidates (Tau/Gly ratio) are not fully understood. In the present study, the stability of taurine- and glycine-conjugated bile acid N-acyl amidates during intestinal transit and absorption was examined to investigate the contribution of intestinal deconjugation to the Tau/Gly ratio in rat bile. Radiolabeled chenodeoxycholic acid (CDC) and its N-acyl amidates with glycine (CDC-Gly) or taurine (CDC-Tau) were introduced into the lumen of the upper small intestine in the biliary fistula rats, and radioactive metabolites in bile, blood, urine, and tissues were identified and quantitated by high-performance liquid chromatography. Results indicated that 1) extensive deconjugation of CDC-Gly occurs during intestinal absorption; 2) CDC-Tau is recovered in bile largely intact; and 3) newly synthesized CDC-Tau and CDC-Gly are formed in a ratio of less than 2:1 after administration of [14C]-CDC. In summary, the present study demonstrates that resistance of taurine-conjugated bile acid N-acyl amidates to hydrolysis in the intestine, rather than a difference in synthesis of taurine- and glycine-conjugated N-acyl amidates in liver, may account for the high Tau/Gly ratio in rat bile.

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Effect of Bile Acids and Other Factors on Cholesterol Uptake by Inverted Intestinal Sacs

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1958.195.3.773 ◽

1958 ◽

Vol 195 (3) ◽

pp. 773-778 ◽

Cited By ~ 6

Author(s):

Archie L. Smith ◽

C. R. Treadwell

Keyword(s):

Small Intestine ◽

Bile Acid ◽

Bile Acids ◽

Binding Sites ◽

Cellular Protein ◽

Rat Small Intestine ◽

Cholesterol Uptake ◽

Quantitative Studies ◽

Mucosal Cells ◽

Intestinal Mucosal Cells

Conditions for the use of inverted sacs of rat small intestine for quantitative studies of cholesterol uptake are described. The uptake of cholesterol by sacs did not require glucose in the incubation medium. Albumin aided cholesterol uptake but was not obligatory for this process. A binding of cholesterol to a cellular protein is proposed as the mechanism for the entrance of cholesterol into intestinal mucosal cells. Both conjugated and unconjugated bile acids inhibited cholesterol uptake possibly by blocking the binding sites of the protein responsible for cholesterol uptake. Commercial taurocholate and glycocholate contain an inhibitor of cholesterol uptake other than the bile acid.

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The expanding role of the bile acid receptor FXR in the small intestine

Journal of Hepatology ◽

10.1016/j.jhep.2006.03.006 ◽

2006 ◽

Vol 44 (6) ◽

pp. 1213-1215 ◽

Cited By ~ 10

Author(s):

Bertrand Cariou ◽

Bart Staels

Keyword(s):

Small Intestine ◽

Bile Acid ◽

Acid Receptor ◽

Bile Acid Receptor

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Nitric oxide modulates T-lymphocyte migration in Peyer's patches and villous submucosa of rat small intestine

Gastroenterology ◽

10.1016/s0016-5085(98)70141-6 ◽

1998 ◽

Vol 115 (3) ◽

pp. 618-627 ◽

Cited By ~ 20

Author(s):

Ryota Hokari ◽

Soichiro Miura ◽

Hitoshi Fujimori ◽

Yoshikazu Tsuzuki ◽

Takeharu Shigematsu ◽

...

Keyword(s):

Nitric Oxide ◽

Small Intestine ◽

T Lymphocyte ◽

Peyer's Patches ◽

Peyer’S Patches ◽

Rat Small Intestine ◽

Lymphocyte Migration

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Bacterial Flora of the Small Intestine and Bile Acid Metabolism in Patients with Hepatico-Jejunostomy Roux-en-Y

Scandinavian Journal of Gastroenterology ◽

10.3109/00365527709181360 ◽

1977 ◽

Vol 12 (8) ◽

pp. 977-982 ◽

Cited By ~ 13

Author(s):

M. Lykkegaard Nielsen ◽

T. Justesen ◽

K. Lenz ◽

O. Vagn Nielsen ◽

S. Lindkaer Jensen

Keyword(s):

Small Intestine ◽

Bile Acid ◽

Acid Metabolism ◽

Bacterial Flora ◽

Bile Acid Metabolism

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Senescence-associated decline of lymphocyte migration in gut-associated lymphoid tissues of rat small intestine

Mechanisms of Ageing and Development ◽

10.1016/j.mad.2003.11.014 ◽

2004 ◽

Vol 125 (3) ◽

pp. 191-199 ◽

Cited By ~ 11

Author(s):

Takashi Ogino ◽

Soichiro Miura ◽

Shunsuke Komoto ◽

Yuriko Hara ◽

Ryota Hokari ◽

...

Keyword(s):

Small Intestine ◽

Lymphoid Tissues ◽

Rat Small Intestine ◽

Lymphocyte Migration

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The effect of dietary fibre on bile acid metabolism in rats

British Journal Of Nutrition ◽

10.1079/bjn19800069 ◽

1980 ◽

Vol 43 (1) ◽

pp. 101-106 ◽

Cited By ~ 11

Author(s):

W. G. Brydon ◽

K. Tadessea ◽

M. A. Eastwood ◽

Margaret E. Lawson

Keyword(s):

Small Intestine ◽

Bile Acid ◽

Acid Metabolism ◽

Male Rats ◽

Bile Acid Metabolism ◽

Fibre Diet ◽

High Fibre ◽

Pelleted Diet ◽

Qualitative Pattern ◽

Microsomal Cholesterol

1. Forty-eight male rats were fed sequentially for 14 d periods on diets containing different fibre contents.2. One of the high-fibre diets was a commercial pelleted diet. The other was a low-fibre diet supplemented with 200 g wheat bran/kg.3. At the end of each feeding period eight rats were killed. Liver microsomal cholesterol 7α-hydroxylase (EC 1.14.1.-) activity and bile acid content of small intestine and colon were determined.4. The different diets did not significantly alter the total intestinal bile acids, but affected the distribution and qualitative pattern in the colon and small intestine.5. On the high-fibre diets deoxycholate, and hyodeoxycholate tended to be increased.6. On the low-fibre diets the α-, β- and ω-muricholic acids tended to be increased.7. Liver microsomal cholesterol 7α-hydroxylase activity was lower in rats fed on the low-fibre and bran- supplemented low-fibre diets compared with that in rats fed on the commercial pelleted diet.

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