AsaGEI2d: a new variant of a genomic island identified in a group of Aeromonas salmonicida subsp. salmonicida isolated from France, which bears the pAsa7 plasmid

2021 ◽  
Author(s):  
Antony T Vincent ◽  
Laurent Intertaglia ◽  
Victor Loyer ◽  
Valérie E Paquet ◽  
Émilie Adouane ◽  
...  
Keyword(s):  
Genomic Island ◽  
Insertion Site ◽  
Aeromonas Salmonicida ◽  
Genomic Islands ◽  
The Other ◽  
Fish Pathogen ◽  
Integrase Gene ◽  
New Variant ◽  
Cat Gene ◽  
Unique Genes

Abstract Genomic islands (Aeromonas salmonicida genomic islands, AsaGEIs) are found worldwide in many isolates of Aeromonas salmonicida subsp. salmonicida, a fish pathogen. To date, five variants of AsaGEI (1a, 1b, 2a, 2b and 2c) have been described. Here, we investigate a sixth AsaGEI, which was identified in France between 2016 and 2019 in 20 A. salmonicida subsp. salmonicida isolates recovered from sick salmon all at the same location. This new AsaGEI shares the same insertion site in the chromosome as the other AsaGEI2s as they all have a homologous integrase gene. This new AsaGEI was thus named AsaGEI2d, and has 5 unique genes compared to the other AsaGEIs. The isolates carrying AsaGEI2d also bear the plasmid pAsa7, which was initially found in an isolate from Switzerland. This plasmid provides resistance to chloramphenicol thanks to a cat gene. This study reveals more about the diversity of the AsaGEIs.

scholarly journals Evidence for a Hybrid Genomic Island in Verocytotoxin-Producing Escherichia coli CL3 (Serotype O113:H21) Containing Segments of EDL933 (Serotype O157:H7) O Islands 122 and 48

2004 ◽  
Vol 72 (3) ◽  
pp. 1496-1503 ◽  
Author(s):  
Songhai Shen ◽  
Mariola Mascarenhas ◽  
Kris Rahn ◽  
James B. Kaper ◽  
Mohamed A. Karmal
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Genomic Island ◽  
Virulence Gene ◽  
Genomic Islands ◽  
The Other ◽  
Middle Portion ◽  
Uremic Syndrome ◽  
The Right ◽  
Vtec Strain

ABSTRACT Genomic O island 122 (OI-122) of the verocytotoxin-producing Escherichia coli (VTEC) strain EDL933 contains four putative virulence genes, Z4321, Z4326, Z4332, and Z4333. However, strain CL3 (serotype O113:H21) contains only Z4321, not the other three genes. To determine whether Z4321 is part of a different genomic island in CL3, a region of 27,293 bp up- and downstream of Z4321 was sequenced and found to contain elements of two different EDL933 genomic islands (OI-48 and OI-122) and a Yersinia pestis-like hemolysin/adhesin gene cluster. The region contained OI-48 genes Z1635, Z1636, and Z1637 at the left terminus and Z1641, Z1642, Z1643, and Z1644 at the right. The middle portion consisted of OI-48 gene Z1640, which was separated into three fragments by genomic segments including the Y. pestis cluster and EDL933 OI-122 genes Z4322, Z4321, and Z4318. In a PCR investigation of 36 VTEC strains of different serotypes, intact Z1640 was present in strains of serotypes O157:H7, O26:H11, O103:H2, O111:NM, and O145:NM, which are associated with hemolytic uremic syndrome and outbreaks. In contrast, fragmented Z1640 was seen in strains of nonepidemic serotypes, such as O91:H21 and O113:H21, and in animal serotypes that have not been associated with human disease, indicating that Z1640 might be a virulence gene.

scholarly journals Functional Tn5393-Like Transposon in the R Plasmid pRAS2 from the Fish Pathogen Aeromonas salmonicida subspecies salmonicida Isolated in Norway

2000 ◽  
Vol 66 (12) ◽  
pp. 5533-5535 ◽  
Author(s):  
Trine M. L'Ab�e-Lund ◽  
Henning S�rum
Keyword(s):  
Ecological Niche ◽  
Aeromonas Salmonicida ◽  
Fish Pathogen ◽  
Agricultural Habitat ◽  
First Time

ABSTRACT Tn5393c containing strA-strB was identified as part of R plasmid pRAS2 from the fish pathogen Aeromonas salmonicida subsp. salmonicida. This is the first time an intact and active transposon in the Tn5393 family has been reported in an ecological niche other than an agricultural habitat.

scholarly journals Completed Genomic Sequence of Bacillus thuringiensis HER1410 Reveals a Cry-Containing Chromosome, Two Megaplasmids, and an Integrative Plasmidial Prophage

2020 ◽  
Vol 10 (9) ◽  
pp. 2927-2939
Author(s):  
Ana Lechuga ◽  
Cédric Lood ◽  
Margarita Salas ◽  
Vera van Noort ◽  
Rob Lavigne ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Replication Origin ◽  
Genomic Island ◽  
Genomic Sequence ◽  
The Other ◽  
Conjugative Plasmid ◽  
Taxonomic Feature ◽  
Sporulation Process ◽  
Long Read

Abstract Bacillus thuringiensis is the most used biopesticide in agriculture. Its entomopathogenic capacity stems from the possession of plasmid-borne insecticidal crystal genes (cry), traditionally used as discriminant taxonomic feature for that species. As such, crystal and plasmid identification are key to the characterization of this species. To date, about 600 B. thuringiensis genomes have been reported, but less than 5% have been completed, while the other draft genomes are incomplete, hindering full plasmid delineation. Here we present the complete genome of Bacillus thuringiensis HER1410, a strain closely related to B. thuringiensis entomocidus and a known host for a variety of Bacillus phages. The combination of short and long-read techniques allowed fully resolving the genome and delineation of three plasmids. This enabled the accurate detection of an unusual location of a unique cry gene, cry1Ba4, located in a genomic island near the chromosome replication origin. Two megaplasmids, pLUSID1 and pLUSID2 could be delineated: pLUSID1 (368 kb), a likely conjugative plasmid involved in virulence, and pLUSID2 (156 kb) potentially related to the sporulation process. A smaller plasmidial prophage pLUSID3, with a dual lifestyle whose integration within the chromosome causes the disruption of a flagellar key component. Finally, phylogenetic analysis placed this strain within a clade comprising members from the B. thuringiensis serovar thuringiensis and other serovars and with B. cereus s. s. in agreement with the intermingled taxonomy of B. cereus sensu lato group.

scholarly journals AsaGEI2b: a new variant of a genomic island identified in theAeromonas salmonicidasubsp.salmonicidaJF3224 strain isolated from a wild fish in Switzerland

2015 ◽  
Vol 362 (13) ◽  
pp. fnv093 ◽  
Author(s):  
Jean-Guillaume Emond-Rheault ◽  
Antony T. Vincent ◽  
Mélanie V. Trudel ◽  
Joachim Frey ◽  
Michel Frenette ◽  
...  
Keyword(s):  
Genomic Island ◽  
Wild Fish ◽  
New Variant

scholarly journals Three-dimensional structure of an open form of the surface layer from the fish pathogen Aeromonas salmonicida.

1989 ◽  
Vol 171 (1) ◽  
pp. 190-197 ◽  
Author(s):  
J S Dooley ◽  
H Engelhardt ◽  
W Baumeister ◽  
W W Kay ◽  
T J Trust
Keyword(s):  
Surface Layer ◽  
Three Dimensional ◽  
Aeromonas Salmonicida ◽  
Open Form ◽  
Dimensional Structure ◽  
Fish Pathogen ◽  
Three Dimensional Structure

scholarly journals Genomic Islands in the Corynebacterium efficiens Genome

2005 ◽  
Vol 71 (6) ◽  
pp. 3126-3130 ◽  
Author(s):  
Ren Zhang ◽  
Chun-Ting Zhang
Keyword(s):  
Thermal Stability ◽  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Genomic Island ◽  
Genomic Islands ◽  
Aspartate Kinase ◽  
Gene Encoding ◽  
Profile Method ◽  
Adaptive Mutations ◽  
Thermal Stability Of

ABSTRACT Corynebacterium efficiens is a gram-positive nonpathogenic bacterium which can grow and produce glutamate at 40°C or above. By using the cumulative GC profile method, we have identified four genomic islands which have many unifying genomic island-specific features in the C. efficiens genome. The presence of the gene encoding an aspartate kinase in a genomic island helps explain the unexpected low thermal stability of this enzyme; i.e., the adaptive mutations have not occurred extensively due to the recent horizontal gene transfer.

scholarly journals Stability of a Pseudomonas putida KT2440 Bacteriophage-Carried Genomic Island and Its Impact on Rhizosphere Fitness

2012 ◽  
Vol 78 (19) ◽  
pp. 6963-6974 ◽  
Author(s):  
Jose M. Quesada ◽  
María Isabel Soriano ◽  
Manuel Espinosa-Urgel
Keyword(s):  
Pseudomonas Putida ◽  
Genomic Island ◽  
Mobile Element ◽  
Direct Repeat ◽  
Promoter Sequence ◽  
Repeat Sequence ◽  
Genomic Islands ◽  
Pseudomonas Putida Kt2440 ◽  
Bacterial Populations ◽  
Content Type

ABSTRACTThe stability of seven genomic islands ofPseudomonas putidaKT2440 with predicted potential for mobilization was studied in bacterial populations associated with the rhizosphere of corn plants by multiplex PCR. DNA rearrangements were detected for only one of them (GI28), which was lost at high frequency. This genomic island of 39.4 kb, with 53 open reading frames, shows the characteristic organization of genes belonging to tailed phages. We present evidence indicating that it corresponds to the lysogenic state of a functional bacteriophage that we have designated Pspu28. Integrated and rarely excised forms of Pspu28 coexist in KT2440 populations. Pspu28 is self-transmissible, and an excisionase is essential for its removal from the bacterial chromosome. The excised Pspu28 forms a circular element that can integrate into the chromosome at a specific location,attsites containing a 17-bp direct repeat sequence. Excision/insertion of Pspu28 alters the promoter sequence and changes the expression level of PP_1531, which encodes a predicted arsenate reductase. Finally, we show that the presence of Pspu28 in the lysogenic state has a negative effect on bacterial fitness in the rhizosphere under conditions of intraspecific competition, thus explaining why clones having lost this mobile element are recovered from that environment.

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