scholarly journals Genetic Architecture of Testis and Seminal Vesicle Weights in Mice

Genetics ◽  
2001 ◽  
Vol 158 (1) ◽  
pp. 333-340 ◽  
Author(s):  
Isabelle Le Roy ◽  
Sylvie Tordjman ◽  
Danièle Migliore-Samour ◽  
Hervé Degrelle ◽  
Pierre L Roubertoux
Keyword(s):  
Seminal Vesicle ◽  
Inbred Strains ◽  
Genome Scan ◽  
Reproductive Organ ◽  
Seminal Vesicles ◽  
Total Variance ◽  
Chromosome 4 ◽  
Lod Score ◽  
Quantitative Genetic ◽  
Testicular Weight

Abstract Comparisons across 13 inbred strains of laboratory mice for reproductive organ (paired seminal vesicles and paired testes) weights indicated a very marked contrast between the C57BL/6By and NZB/BINJ mice. Subsequently these strains were selected to perform a quantitative genetic analysis and full genome scan for seminal vesicle and testis weights. An F2 population was generated. The quantitative genetic analyses indicated that each was linked to several genes. Sixty-six short sequences for length polymorphism were used as markers in the wide genome scan strategy. For weight of paired testes, heritability was 82.3% of the total variance and five QTL contributed to 72.8% of the total variance. Three reached a highly significant threshold (>4.5) and were mapped on chromosome X (LOD score 9.11), chromosome 4 (LOD score 5.96), chromosome 10 (LOD score 5.81); two QTL were suggested: chromosome 13 (LOD score 3.10) and chromosome 18 (LOD score 2.80). Heritability for weight of seminal vesicles was 50.7%. One QTL was mapped on chromosome 4 (LOD score 9.21) and contributed to 24.2% of the total variance. The distance of this QTL to the centromere encompassed the distance of the QTL linked with testicular weight on chromosome 4, suggesting common genetic mechanisms as expected from correlations in the F2. Both testis and seminal vesicle weights were associated with a reduction in the NZB/BINJ when this strain carried the YNPAR from CBA/H whereas the YNPAR from NZB/BINJ in the CBA/H strain did not modify reproductive organ weights, indicating that the YNPAR interacts with the non-YNPAR genes. The effects generated by this chromosomal region were significant but small in size.

DIFFERENCES BETWEEN MOUSE STRAINS IN TESTICULAR CHOLESTEROL LEVELS AND ANDROGEN TARGET ORGANS

1972 ◽  
Vol 55 (1) ◽  
pp. 173-184 ◽  
Author(s):  
A. BARTKE ◽  
J. G. M. SHIRE
Keyword(s):  
Inbred Mice ◽  
Strain Differences ◽  
Inbred Strains ◽  
Seminal Vesicles ◽  
Cholesterol Concentration ◽  
Mouse Strains ◽  
Cholesterol Levels ◽  
Target Organs ◽  
Testicular Weight ◽  
Androgenic Steroids

SUMMARY The concentrations of esterified and free cholesterol in the testes, and the weights of the testes, seminal vesicles, kidneys and submandibular glands, were determined in mice of the C57BL/10J and DBA/2J inbred strains. Measurements were also made on reciprocal F1 hybrids. Cholesterol concentration and the weights of the testes and seminal vesicles were also studied in C57BL/6J and AKR/J inbred mice and in mice from two random-bred stocks. Considerable strain differences were found both in the concentration of esterified cholesterol and in gonadal weight. Since all the animals were maintained under the same environmental conditions the differences must have been genetic in origin. The decrease in absolute testicular weight which occurred in the C57BL/10 mice after the age of 8 weeks was accompanied by an increase in the percentage of total cholesterol present as ester. Groups of C57BL/10 males were treated with luteinizing hormone (LH), follicle-stimulating hormone (FSH), prolactin-secreting pituitary isografts, pregnant mare serum gonadotrophin (PMS), testosterone propionate or pregnenolone. The concentration of esterified cholesterol was slightly decreased by LH and increased by pituitary grafts, confirming earlier studies in other strains. Treatment with PMS caused a drastic depletion of the high levels of esterified cholesterol usually found in the testes of these mice, and also caused marked growth of the seminal vesicles. Testosterone increased the weight of seminal vesicles while pregnenolone had no effect. None of the treatments stimulated spermatogenesis. A marked sex difference in the weight of the kidneys was absent in the C57BL/10 strain but was present in the DBA/2 strain. The kidneys of C57BL/10 males were significantly smaller than those of DBA/2 males. These findings, together with other studies on C57BL/10 mice, suggest that males of this strain are relatively androgen deficient. This could be due to inadequate production of FSH and LH, perhaps accompanied by a deficiency in the conversion of pregnenolone to androgenic steroids. The effect of the C57BL/10 genotype on spermatogenesis either is not hormone mediated or else is irreversible after the age of 6 weeks.

Electron Microscopic Study of the Epithelium of the Seminal Vesicle of Aging Rats

1974 ◽  
Vol 32 ◽  
pp. 138-139
Author(s):  
V. F. Allison ◽  
G. C. Fink ◽  
G. W. Cearley
Keyword(s):  
Cell Growth ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Seminal Vesicle ◽  
Seminal Vesicles ◽  
Epithelial Layer ◽  
Epithelial Hyperplasia ◽  
Electron Microscopic ◽  
Aging Rats ◽  
Pseudostratified Epithelium

It is well known that epithelial hyperplasia (benign hypertrophy) is common in the aging prostate of dogs and man. In contrast, little evidence is available for abnormal epithelial cell growth in seminal vesicles of aging animals. Recently, enlarged seminal vesicles were reported in senescent mice, however, that enlargement resulted from increased storage of secretion in the lumen and occurred concomitant to epithelial hypoplasia in that species.The present study is concerned with electron microscopic observations of changes occurring in the pseudostratified epithelium of the seminal vescles of aging rats. Special attention is given to certain non-epithelial cells which have entered the epithelial layer.

Histologic and Histochemical Characterization of Seminal Vesicle Intraluminal Secretions

2001 ◽  
Vol 125 (1) ◽  
pp. 141-145
Author(s):  
Rajal B. Shah ◽  
Min W. Lee ◽  
Alvaro A. Giraldo ◽  
Mahul B. Amin
Keyword(s):  
Seminal Vesicle ◽  
Alcian Blue ◽  
Prostate Carcinoma ◽  
Periodic Acid ◽  
Prostate Gland ◽  
Seminal Vesicles ◽  
Periodic Acid Schiff ◽  
Associated Malignancy ◽  
Distinguishing Features

Abstract Context.—We have observed intraluminal crystalloid morphology in seminal vesicles that is superficially similar to that seen in prostate neoplasia, but found little information on such morphology in the literature. Design.—Two hundred fifty-three prostate specimens (163 needle biopsies, 75 radical prostatectomies with prostate carcinoma, 11 prostates from autopsy, and 4 cystoprostatectomies without prostate carcinoma) were examined for seminal vesicle secretions, which were categorized as (a) dense platelike inspissated, (b) fluidlike, (c) crystalloid morphology, and (d) absent. Histochemical stains (periodic acid–Schiff with and without diastase, Alcian blue at pH 2.5, and mucicarmine) were performed to characterize the nature of secretions. Results.—Proteinaceous secretions were identified in 82% of seminal vesicles examined. Of these, 61% had predominantly dense, platelike, inspissated secretions, 15% had predominantly fluidlike secretions, and 24% had predominantly crystalloid morphology. Although in some cases the crystalloid morphology resembled that of prostatic intraluminal crystalloids, the seminal vesicle crystalloids differed in that they were invariably multiple, had curved edges, and had varied forms (elliptical, cylindrical, rodlike, and rectangular). Seventy-one percent of seminal vesicle crystalloids were associated with dense, platelike, inspissated secretions and appeared to be created by fracturing within platelike secretions. There was no relationship between seminal vesicle crystalloid morphology and associated malignancy in the prostate gland, as it was seen in 24% of cases with prostate carcinoma and 25% of cases without prostate carcinoma (P = 1.0000). Fluidlike secretions were positive for Alcian blue (pH 2.5) and mucicarmine, whereas dense platelike secretions and crystalloid morphology were negative for Alcian blue (pH 2.5) and mucicarmine. Conclusions.—Seminal vesicle secretions are fairly common and, when fluidlike, are composed of acid mucopolysaccharides. Inspissation of secretions appears to be associated with loss of acidity, presumably resulting in dense platelike secretions and crystallization. Awareness of both the crystalloid morphology in seminal vesicle tissue and the distinguishing features from prostatic crystalloids may be important while interpreting prostate needle biopsies in which seminal vesicle epithelium may be confused for prostate carcinoma because of a small acinar morphology with accompanying cytologic atypia and crystalloid morphology.

Serum alkaline phosphatase activity is regulated by a chromosomal region containing the alkaline phosphatase 2 gene (Akp2) in C57BL/6J and DBA/2J mice

2005 ◽  
Vol 23 (3) ◽  
pp. 295-303 ◽  
Author(s):  
Jennifer E. Foreman ◽  
David A. Blizard ◽  
Glenn Gerhard ◽  
Holly A. Mack ◽  
Dean H. Lang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Chromosomal Region ◽  
Serum Alkaline Phosphatase ◽  
Inbred Strains ◽  
Lod Score ◽  
Serum Alkaline Phosphatase Activity ◽  
Single Nucleotide ◽  
Significance Level ◽  
Hepatic Expression ◽  
Trait Locus

Quantitative trait locus (QTL) analyses were conducted to identify chromosomal regions that contribute to variability in serum alkaline phosphatase (AP) enzyme activity in mice derived from the C57BL/6J (B6) and DBA/2J (D2) inbred strains. Serum AP was measured in 400 B6D2 F2 mice at 5 mo and 400 B6D2 F2 mice at 15 mo of age that were genotyped at 96 microsatellite markers, and in 19 BXD recombinant inbred (RI) strains at 5 mo of age. A QTL on the distal end of chromosome 4 was present in all sex- and age-specific analyses with a peak logarithm of odds (LOD) score of 20.36 at 58.51 cM. The Akp2 gene, which encodes the major serum AP isozyme, falls within this QTL region at 70.2 cM where the LOD score reached 13.2 (LOD significance level set at 4.3). Serum AP activity was directly related to the number of D2 alleles of a single nucleotide polymorphism in the 5′-flanking region of the Akp2 gene, although no strain-related differences in hepatic expression of Akp2 RNA were found. A variety of sequence polymorphisms in this chromosomal region could be responsible for the differences in serum AP activity; the Akp2 gene, however, with several known amino acid substitutions between protein sequences of the B6 and D2 strains, is a leading candidate.

Induction of functional cytodifferentiation in the epithelium of tissue recombinants. I. Homotypic seminal vesicle recombinants

Development ◽  
1989 ◽  
Vol 106 (2) ◽  
pp. 219-234 ◽  
Author(s):  
S.J. Higgins ◽  
P. Young ◽  
J.R. Brody ◽  
G.R. Cunha
Keyword(s):  
Seminal Vesicle ◽  
Time Course ◽  
Full Range ◽  
Seminal Vesicles ◽  
Neonatal Rat ◽  
Secretory Proteins ◽  
Functional Variation ◽  
Adult Rats ◽  
Normal Functional

Functional cytodifferentiation of seminal vesicle epithelium was investigated in tissue recombinants. Neonatal rat and mouse seminal vesicles were separated into epithelium and mesenchyme using trypsin. Epithelium and mesenchyme were then recombined in vitro to form interspecific rat/mouse homotypic recombinants. Growth as renal grafts in adult male athymic mice resulted in seminal vesicle morphogenesis in 70% of the recombinants (the remaining 30% failed to grow). Functional cytodifferentiation was judged by the expression of the major androgen-dependent secretory proteins characteristic of the seminal vesicles of adult rats and mice. Antibodies specific for each of these proteins were used to screen tissue sections by immunocytochemistry and to probe protein extracts by immunoblotting techniques. The heterospecific recombinants synthesized the full range of seminal vesicle secretory proteins that typifies the species providing the epithelium of the recombinant, not the mesenchyme. There was little functional variation between individual recombinants. The time course of development corresponded to that of intact neonatal seminal vesicles grown under the same conditions. Morphogenesis and functional cytodifferentiation were not evident after one week, but were well advanced after two weeks. Seminal vesicle recombinants grown for three weeks were indistinguishable morphologically and functionally from normal adult seminal vesicles. In addition, the ability of adult seminal vesicle epithelium to be induced to proliferate was examined. In association with neonatal seminal vesicle mesenchyme, the epithelium of the adult seminal vesicle proliferated and retained its normal functional activity. Thus, seminal vesicle functional cytodifferentiation can be faithfully reproduced in homotypic tissue recombinants. The methods used in this study will be used to investigate seminal vesicle development in instructive inductions of heterotypic epithelia.

Induction of functional cytodifferentiation in the epithelium of tissue recombinants. II. Instructive induction of Wolffian duct epithelia by neonatal seminal vesicle mesenchyme

Development ◽  
1989 ◽  
Vol 106 (2) ◽  
pp. 235-250 ◽  
Author(s):  
S.J. Higgins ◽  
P. Young ◽  
G.R. Cunha
Keyword(s):  
Seminal Vesicle ◽  
Normal Development ◽  
Seminal Vesicles ◽  
Wolffian Duct ◽  
Secretory Proteins ◽  
Ductus Deferens ◽  
Tissue Sections ◽  
Duct Epithelium ◽  
Mouse Tissues ◽  
Renal Grafts

When grown as renal grafts in adult male hosts, the upper (cranial), middle and lower (caudal) portions of fetal mouse and rat Wolffian ducts developed into epididymis, epididymis plus ductus deferens, and seminal vesicle, respectively. In heterotypic tissue recombinants, the epithelia from upper and middle Wolffian ducts were instructively induced to undergo seminal vesicle morphogenesis by neonatal seminal vesicle mesenchyme. Functional cytodifferentiation was examined in these recombinants using antibodies against major androgen-dependent, seminal vesicle-specific secretory proteins. The instructively induced Wolffian duct epithelia synthesized normal amounts of all of the secretory proteins characteristic of mature seminal vesicles, as judged by immunocytochemistry on tissue sections and gel electrophoresis plus immunoblotting of secretions extracted from the recombinants. In heterospecific recombinants composed of rat and mouse tissues, the seminal vesicle proteins induced were specific for the species that had provided the epithelium. This showed that the seminal vesicle epithelium in the recombinants was derived from instructively induced Wolffian duct epithelium and not from epithelial contamination of the mesenchymal inductor. Upper Wolffian duct epithelium, instructively induced to undergo seminal vesicle morphogenesis, did not express epididymis-specific secretory proteins, showing that its normal development had been simultaneously repressed.

scholarly journals An unusual cause of hematospermia: Primary adenocarcinoma of the seminal vesicle

2012 ◽  
Vol 6 (6) ◽  
pp. 259 ◽  
Author(s):  
Alper Eken ◽  
Volkan Izol ◽  
I. Atilla Aridogan ◽  
Seyda Erdogan ◽  
Arbil Acikalin ◽  
...  
Keyword(s):  
Seminal Vesicle ◽  
Current Literature ◽  
Seminal Vesicles ◽  
Primary Adenocarcinoma

Adenocarcinoma of the seminal vesicles is one of the rare causes of hematospermia. Primary seminal vesicle adenocarcinoma is extremely rare and difficult to diagnose due to frequent invasion of adenocarcinomas of the surrounding organs, especially the prostate. In the present study, a case of a primary seminal vesicle adenocarcinoma will be discussed in the light of the current literature.

scholarly journals Recessive mutation in a standard recombinant-inbred line of mice affects seminal vesicle shape

1988 ◽  
Vol 52 (1) ◽  
pp. 27-32 ◽  
Author(s):  
N. M. Shukri ◽  
F. Grew ◽  
J. G. M. Shire
Keyword(s):  
Seminal Vesicle ◽  
Inbred Line ◽  
Recombinant Inbred Line ◽  
Recombinant Inbred ◽  
Gel Electrophoresis ◽  
Seminal Vesicles ◽  
Recessive Mutation ◽  
External Appearance ◽  
Target Organs ◽  
Vesicle Shape

SummaryA recessive autosomal mutation has been found in the CXBI/ByEss recombinant-inbred line but in neither of the parental strains, C57BL/6ByEss and BALB/cByEss. Its presence in the CXBI/ByJax and CXBI/ByLac sublines suggests an origin early in inbreeding. The locus, seminal vesicle shape (svs), appears to be linked to the albino locus on chromosome 7. The homozygote has seminal vesicles with a smooth tubular external appearance. In segregating crosses homozygotes had slightly lighter seminal vesicles but the weights of other androgen target organs were not reduced. Exogenous testosterone increased the size of the seminal vesicles but did not alter their shape. The mutation did not affect the pattern of proteins on SDS–acrylamide gel electrophoresis, which did differ between the parental strains. The locus affecting a 27000 Da protein has provisionally been assigned the symbolsvp-4.

Application of AFLP markers for QTL mapping in the rabbit

Genome ◽  
2002 ◽  
Vol 45 (5) ◽  
pp. 914-921 ◽  
Author(s):  
Wim A van Haeringen ◽  
M G. Den Bieman ◽  
Æ Lankhorst ◽  
H A van Lith ◽  
L F.M van Zutphen
Keyword(s):  
Genetic Analysis ◽  
Total Cholesterol ◽  
Dietary Cholesterol ◽  
Relative Weight ◽  
Area Under The Curve ◽  
Backcross Progeny ◽  
Inbred Strains ◽  
Serum Total Cholesterol ◽  
Aflp Markers ◽  
Lod Score

Two rabbit (Oryctolagus cuniculus) inbred strains (AX/JU and IIIVO/JU) have been used for genetic analysis of quantitative traits related to dietary cholesterol susceptibility. Application of the AFLP (amplified fragment length polymorphism) technique with 15 primer combinations revealed 226 polymorphisms between the 2 inbred strains. A total of 57 animals from a backcross progeny (IIIVO/JU × [IIIVO/JU × AX/JU]F1) were available for the genetic analysis. These backcross animals were fed a commercial pelleted diet fortified with 0.3% w/w cholesterol during a test period that lasted five weeks. A male genetic map could be constructed, consisting of 12 linkage groups and 103 AFLP markers. Linkage analysis between the cholesterol-related traits and marker loci revealed a significant LOD score for the relative weight of adrenal glands in males (LOD score = 3.83), whereas suggestive linkages were found for basal serum total cholesterol levels in females (LOD score = 2.69), for serum total cholesterol response (area under the curve) in males (LOD score = 2.21), and for hematocrit in males (LOD score = 3.24).Key words: adrenal, AFLP, cholesterol, hematocrit, QTL, rabbit.

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